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2.1.1.228: tRNA (guanine37-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine37-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.228

Reaction

S-adenosyl-L-methionine
+
guanine37 in tRNA
=
S-adenosyl-L-homocysteine
 +
N1-methylguanine37 in tRNA

Synonyms

At3g56120, aTrm5a methyltransferase, aTrm5a/Taw22-like enzyme, AtTrm5a, bacterial tRNA (guanine37-N1)-methyltransferase, bacterial tRNA-(N1G37) methyltransferase, EC 2.1.1.31, EcTrmD, HiTrmD, HsTrm5, m1G37 tRNA methyltransferase, m1G37-forming tRNA methyltransferase, Mj-Trm5, MJ0883, MjTrm5, MjTrm5b, mono-functional methyltransferase, More, MtbTrmD, NEQ228, PA14_15990, PAB2272, PAB_RS03940, PaTrm5a, PaTrm5b, PaTrmD, SaTrmD, Ta0997, TAW22, transfer RNA (m1G37) methyltransferase, Trm1, TRM5, Trm5a, Trm5a/Taw22-like enzyme, trm5b, Trm5p, TrmD, TRMT5, tRNA (guanine(37)-N1)-methyltransferase 1, tRNA (guanine37-N1)-methyltransferase, tRNA (guanosine-1) methyltransferase, tRNA (m1G37) methyltransferase, tRNA (m1G37)-methyltransferase, tRNA m1G37 methyltransferase, tRNA methyltransferase, tRNA methyltransferase 5, tRNA methyltransferase D, tRNA(m(1)G37)methyltransferase, tRNA(m1G37) methyltransferase, tRNA(m1G37) MTase, tRNA(m1G37)methyltransferase, tRNA-(N1G37) methyltransferase, tRNAPhe:imG2 methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.228 tRNA (guanine37-N1)-methyltransferase

Purification

Purification on EC 2.1.1.228 - tRNA (guanine37-N1)-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, from Escherichia coli by metal ion affinity chromatography and anion exchange chromatography
recombinant enzyme
recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration
recombinant expression of His-tagged wild-type and mutant enzymes to near homogeneity by 20 min heat treatment at 70°C and 65°C, respectively, followed by affinity chromatography and desalting gel filtration
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through 3C protease, another step of nickel affinity chromatography, followed by gel filtration
-
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
recombinant His6-tagged full-length PaTrm5b and PaTrm5b73-330 mutant fromEscherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and dialysis
recombinant His6-tagged wild-type and mutant enzymes
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta T1R by nickel affinity chromatography and gel filtration
recombinant N-terminally His-tagged wild-type and S88L mutant enzymes from Escherichia coli strain SG13009 by nickel affinity chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and heparin affinity chromatography, and dialysis
recombinant protein
-
Trm5p is purified as a fusion protein with maltose-binding protein