2.1.1.260: rRNA small subunit pseudouridine methyltransferase Nep1

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For detailed information about rRNA small subunit pseudouridine methyltransferase Nep1, go to the full flat file.

Word Map on EC 2.1.1.260

2.1.1.260

rrnas

jannaschii

methanocaldococcus

nucleolus

hypermodified

s-adenosylmethionine

archaea

three-hybrid

rna-binding

Reaction

N1-methylpseudouridine1191 in yeast 18S rRNA

Synonyms

Bowen-Conradi syndrome protein, Emg1, Emg1p, essential for mitotic growth 1, N1-specific pseudouridine methyltransferase, Nep1, Nep1 methyltransferase, Nep1p, nucleolar essential protein 1, pseudouridine N1-methyltransferase, pseudouridine-N1-specific methyltransferase

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.260 rRNA small subunit pseudouridine methyltransferase Nep1

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Results	in table
44	AA Sequence
10	Cloned(Commentary)
10	Cofactor
3	Crystallization (Commentary)
1	Expression
35	General Information
1	Inhibitors
12	Localization
2	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
1	Pathway
51	PDB ID
4	pH Optimum
10	Protein Variants
8	Purification (Commentary)
1	Reaction
16	Reference
1	Source Tissue
21	Substrates and Products (Substrate)
9	Subunits
56	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

Purification

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Purification on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1

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native enzyme partially from subcellular fractions by sucrose density gradient fractionation

Saccharomyces cerevisiae

721118

recombinant His-tagged Nep1 from Escherichia coli strain M15(pREP4) by nickel affinity chromatography

Homo sapiens

720582

recombinant His6-tagged Nep1 from Escherichia coli strain RosettaTM-DE3-pLysS by nickel affinity chromatography, cleavage of the tag by TEV protease, and two steps of gel filtration to over 99% purity

Saccharomyces cerevisiae

720589

recombinant N-terminally His6-tagged wild-type and D90G mutant Nep1 from Escherichia coli strain XL1-blue by nickel affinity chromatography and gel filtration

2 entries

recombinant Nep1 by cation exchange chromatography and gel filtration

Methanocaldococcus jannaschii

720582

recombinant Nep1p from Escherichia coli strain BL21(DE3) by ion exchange chromatography and gel filtration

Methanocaldococcus jannaschii

Q57977

720579

recombinant wild-type and selenomethionine-labeled His6-tagged Nep1 from Escherichia coli strain RosettaTM-DE3-pLysS by nickel affinity chromatography, cleavage of the tag by TEV protease, and two steps of gel filtration to over 99% purity

Archaeoglobus fulgidus

O29524

720589