2.1.1.266: 23S rRNA (adenine2030-N6)-methyltransferase
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For detailed information about 23S rRNA (adenine2030-N6)-methyltransferase, go to the full flat file.
Reaction
Synonyms
23S rRNA methyltransferase, adenine-N6 methyltransferase, m6A2030 methyltransferase, ribosomal RNA large subunit methyltransferase J, rlmJ, rRNA m6A methyltransferase, YhiR, YhiR protein
ECTree
2.1.1.266 23S rRNA (adenine2030-N6)-methyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.266 - 23S rRNA (adenine2030-N6)-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
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S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
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S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
nascent 23S rRNA is the natural substrate for YhiR
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S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
adenine-N6 methyltransferase, encoded by gene yhiR, is specific for modification of A2030 of 23S ribosomal RNA. YhiR by itself, without any additional proteins, is able to modify 23S rRNA
mass spectrometric product identification, overview
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S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli
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S-adenosyl-L-methionine + adenine2030 in 23S rRNA
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA
In vitro transcription of 23S rRNA substrate from the wild-type BW25113 strain. RlmJ methylates in vitro transcribed 23S rRNA, as well as a minimal substrate corresponding to helix 72, demonstrating independence of modifications and tertiary interactions in the RNA substrate. RlmJ displays specificity for adenosine, substrate binding site structure, overview. Location of A2030 in Escherichia coli 23S rRNA, overview
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additional information
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enzyme activity with in vitro modified potential YhiR substrates prepared from the enzyme-deficient DELTAyhiR strain: methylation of the 50S subunits, NH4Cl/ethanol split particles, LiCl split particles, and deproteinized 23S rRNA, overview
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additional information
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enzyme activity with in vitro modified potential YhiR substrates prepared from the enzyme-deficient DELTAyhiR strain: methylation of the 50S subunits, NH4Cl/ethanol split particles, LiCl split particles, and deproteinized 23S rRNA, overview
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