2.1.1.270: (+)-6a-hydroxymaackiain 3-O-methyltransferase
This is an abbreviated version!
For detailed information about (+)-6a-hydroxymaackiain 3-O-methyltransferase, go to the full flat file.
Reaction
Synonyms
(+)-6a-hydroxymaackiain 3-O-methyltransferase 1, 6a-HMK methyltransferase, 6a-hydroxymaackiain methyltransferase, HI4'OMT/HM3OMT, HMM1, HMM2, hmm6, hydroxymaackiain methyltransferase, IOMT8, S-adenosyl-L-methionine: 2-hydroxyisoflavanone 4'-O-methyltransferase
ECTree
2.1.1.270 (+)-6a-hydroxymaackiain 3-O-methyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.270 - (+)-6a-hydroxymaackiain 3-O-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (+)-maackiain
S-adenosyl-L-homocysteine + ?
at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
the bifunctional enzyme also catalyzes the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanonewith slightly higher catalytic efficiency
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
isoform HMM1 shows efficiencies for the methylation of 2,7,4'-trihydroxyisoflavanone 4times higher than for the methylation of (+)-6a-hydroxymaackiain, substrate of 6a-hydroxymaackiain-3-O-methyltransferase. Isoform HMM2 has a higher Vmax and lower Km on (+)-6a-hydroxymaackiain, and has a 67fold higher efficiency for the methylation of (+)-6a-hydroxymaackiain than that for 2,7,4'-trihydroxyisoflavanone
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
isoform HMM2, 6a-hydroxymaackiain is the preferred substrate
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
HMM2 is involved primarily in the biosynthesis of (+)-pisatin
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
-
HMM2 shows efficiencies (relative Vmax/Km) for the 3-O-methylation of (+)-6a-hydroxymaackiain 67 times higher than for the 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone
-
-
?
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
Pisum sativum UWA H2012-81
-
-
-
?
additional information
?
-
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
-
-
?
additional information
?
-
-
in Pisum sativum, there are two highly similar hydroxymaackiain 3-O-methyltransferases HMM, with HMM1 prefering 2,7,4'-trihydroxyisoflavanone as a substrate and HMM2 being the specific O-methyltransferase that methylates (+)-6a-hydroxymaackiain
-
-
?
