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2.1.1.273: benzoate O-methyltransferase

This is an abbreviated version!
For detailed information about benzoate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.273

Reaction

S-adenosyl-L-methionine
+
benzoate
=
S-adenosyl-L-homocysteine
+
methyl benzoate

Synonyms

AlBSMT1, AtBSMT1, BAMT, benzoate-salicylate methyltransferase, benzoic acid carboxyl methyl transferase, benzoic acid carboxyl methyltransferase, benzoic acid carboxymethyl transferase, benzoic acid/SA methyltransferase, benzoic acid/salicylic acid carboxyl methyltransferase, benzoic acid/salicylic acid methyltransferase, BSMT, BSMT1, class II SABATH protein, More, OMT8, PbBSMT methyltransferase, PtSABATH24, S-adenosyl-l-methionine: benzoicacid carboxyl methyl transferase, S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase, S-adenosyl-L-methionine:benzoic acid carboxyl methyltransferase, S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, SA MTase, SABATH methyltransferase 4, SABATH24, salicylic acid methyltransferase, SAM:benzoic acid carboxyl methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.273 benzoate O-methyltransferase

Purification

Purification on EC 2.1.1.273 - benzoate O-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by nickel-based affinity chromatography
enzyme purified from upper and lower petal lobes of 5- to 10-day-old snapdragon flowers using DE53 anion exchange chromatography, hydrophobic interaction chromatography using Phenyl-Sepharose 6FF, and Mono-Q chromatography
-
from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography