2.1.1.28: phenylethanolamine N-methyltransferase
This is an abbreviated version!
For detailed information about phenylethanolamine N-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.28
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2.1.1.28
-
catecholamine
-
medulla
-
hydroxylase
-
adrenergic
-
dopamine
-
chromaffin
-
beta-hydroxylase
-
glucocorticoid
-
sympathetic
-
rostrally
-
catecholaminergic
-
noradrenergic
-
innervation
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ventrolateral
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hypothalamic
-
corticosterone
-
catecholamine-synthesizing
-
oblongata
-
dopamine-beta-hydroxylase
-
pnmt-immunoreactive
-
perikarya
-
tractus
-
adrenomedullary
-
proenkephalin
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dorsomedial
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sympathoadrenal
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intermediolateral
-
alpha2-adrenoceptor
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1,2,3,4-tetrahydroisoquinoline
-
coeruleus
-
phenylethanolamine-n-methyltransferase
-
postrema
-
pnmt-ir
-
medicine
-
bulbospinal
-
normetanephrine
-
solitarii
-
analysis
- 2.1.1.28
- catecholamine
- medulla
- hydroxylase
-
adrenergic
- dopamine
-
chromaffin
- beta-hydroxylase
- glucocorticoid
-
sympathetic
-
rostrally
-
catecholaminergic
-
noradrenergic
-
innervation
-
ventrolateral
- hypothalamic
- corticosterone
-
catecholamine-synthesizing
- oblongata
- dopamine-beta-hydroxylase
-
pnmt-immunoreactive
- perikarya
-
tractus
-
adrenomedullary
- proenkephalin
-
dorsomedial
-
sympathoadrenal
-
intermediolateral
-
alpha2-adrenoceptor
- 1,2,3,4-tetrahydroisoquinoline
-
coeruleus
-
phenylethanolamine-n-methyltransferase
-
postrema
-
pnmt-ir
- medicine
-
bulbospinal
- normetanephrine
-
solitarii
- analysis
Reaction
Synonyms
hPNMT, methyltransferase, noradrenaline N-, NMT, noradrenalin methyltransferase, noradrenalin N-methyltransferase, noradrenaline N-methyltransferase, norepinephrin N-methyltransferase, norepinephrine methyltransferase, norepinephrine N-methyltransferase, phenethanolamine methyltransferase, phenethanolamine N-methyltransferase, PNMT
ECTree
2.1.1.28 phenylethanolamine N-methyltransferaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.1.1.28 - phenylethanolamine N-methyltransferase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0053
cis-(1R,2S)-2-amino-1-tetralol
wild type enzyme, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.00055
3-trifluoromethylphenylethanolamine
wild type enzyme, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine
0.055
3-trifluoromethylphenylethanolamine
mutant D267, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine
0.0015
anti-9-amino-6-(trifluoromethyl)benzonorbornene
wild type enzyme, assay containing S-adenosyl-L-methionine and anti-9-amino-6-(trifluoromethyl)benzonorbornene
0.025
anti-9-amino-6-(trifluoromethyl)benzonorbornene
mutant D267A, assay containing S-adenosyl-L-methionine and anti-9-amino-6-(trifluoromethyl)benzonorbornene
0.0055
octopamine
wild type enzyme, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.095
phenylethanolamine
-
purified in the absence of reducing agent, dimer, His-tagged
0.099
phenylethanolamine
wild type enzyme, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.1
phenylethanolamine
wild type enzyme, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.101
phenylethanolamine
mutant E185Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.102
phenylethanolamine
mutant E185A, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.179
phenylethanolamine
mutant E219Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.32
phenylethanolamine
mutant E185D, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.58
phenylethanolamine
mutant E219A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
1.31
phenylethanolamine
mutant K57A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
1.63
phenylethanolamine
mutant V53A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
1.64
phenylethanolamine
mutant D267N, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
1.73
phenylethanolamine
mutant Y35F, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
2.05
phenylethanolamine
mutant D267A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.0012
S-adenosyl-L-methionine
wild type enzyme, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine
0.0012
S-adenosyl-L-methionine
cosubstrate 3-trifluoromethylphenylethanolamine, pH 8.0, 30°C
0.0013
S-adenosyl-L-methionine
mutant E185Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.0017
S-adenosyl-L-methionine
mutant E185A, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.002
S-adenosyl-L-methionine
wild type enzyme, assay containing S-adenosyl-L-methionine and anti-9-amino-6-(trifuoromethyl)benzonorbornene
0.0024
S-adenosyl-L-methionine
mutant D267, assay containing S-adenosyl-L-methionine and 3-trifluoromethylphenylethanolamine
0.0034
S-adenosyl-L-methionine
wild type enzyme, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.0034
S-adenosyl-L-methionine
wild type enzyme, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.0034
S-adenosyl-L-methionine
cosubstrate phenylethanolamine, pH 8.0, 30°C
0.0035
S-adenosyl-L-methionine
-
purified in the absence of reducing agent, dimer, His-tagged
0.0051
S-adenosyl-L-methionine
mutant D267A, assay containing S-adenosyl-L-methionine and anti-9-amino-6-(trifluoromethyl)benzonorbornene
0.0067
S-adenosyl-L-methionine
mutant E219Q, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.0071
S-adenosyl-L-methionine
mutant E219A and mutant D267N, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.0081
S-adenosyl-L-methionine
mutant D267A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.0096
S-adenosyl-L-methionine
mutant V53A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.0117
S-adenosyl-L-methionine
mutant K57A, radiochemical assay containing S-adenosyl-L-methionine and phenylethanolamine
0.014
S-adenosyl-L-methionine
mutant E185D, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
0.045
S-adenosyl-L-methionine
mutant Y35F, assay mixture consists of 25 microl of 0.5 M phosphate buffer (pH 8.0), 5 microl of [methyl-3H]S-adenosyl-L-methionine containing approximately 2.5 X 10 00000 dpm, varying amounts of phenylethanolamine and unlabeled S-adenosyl-L-methionine, 20 microl enzyme and water at 30°C.
