2.1.1.281: phenylpyruvate C3-methyltransferase
This is an abbreviated version!
For detailed information about phenylpyruvate C3-methyltransferase, go to the full flat file.
Reaction
Synonyms
C-MT, ELQ87_35210, MppJ, MT SgvM, phenylpyruvate Cbeta-methyltransferase, phenylpyruvate methyltransferase, S-adenosylmethionine-dependent methyltransferase, SgvM
ECTree
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Engineering
Engineering on EC 2.1.1.281 - phenylpyruvate C3-methyltransferase
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C319A
site-directed mutagenesis, the mutant shows 39% reduced activity compared to the wild-type enzyme
D244E
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
D244L
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
R127L/D244E
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
W99F
the mutant shows altered stereochemistry in the reaction compared to the wild-type enzyme
C319A
-
site-directed mutagenesis, the mutant shows 39% reduced activity compared to the wild-type enzyme
-
D244E
-
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
-
D244L
-
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
-
R127L/D244E
-
site-directed mutagenesis, the mutant enzyme shows altered conformation and substrate binding structure compared to the wild-type enzyme
-
W99F
-
the mutant shows altered stereochemistry in the reaction compared to the wild-type enzyme
-