2.1.1.282: tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase
This is an abbreviated version!
For detailed information about tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase, go to the full flat file.
Reaction
Synonyms
aTrm5a methyltransferase, aTrm5a-type enzyme, aTrm5a/Taw22-like enzyme, More, NEQ228, PAB2272, PaTrm5a, SSO2439, Taw21, TAW22, Trm5a, Trm5a/Taw22-like enzyme, tRNAPhe:imG2 methyltransferase, TYW3, YGL050w
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.282 - tRNAPhe 7-[(3-amino-3-carboxypropyl)-4-demethylwyosine37-N4]-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine
S-adenosyl-L-homocysteine + wyosine
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-demethyl]wyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)-4-methyl]wyosine37 in tRNAPhe
the enzyme is involved in the biosynthesis of the tricyclic base wybutosine
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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?
S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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?
S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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?
S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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?
S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
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S-adenosyl-L-methionine + 4-demethylwyosine
S-adenosyl-L-homocysteine + isowyosine
i.e. im-G14, activity of EC 2.1.1.282
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S-adenosyl-L-homocysteine + wyosine
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S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine
S-adenosyl-L-homocysteine + wyosine
i.e. yW-86, activity of EC 2.1.1.228
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S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine
S-adenosyl-L-homocysteine + wyosine
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S-adenosyl-L-methionine + 7-aminocarboxypropyl-demethylwyosine
S-adenosyl-L-homocysteine + wyosine
i.e. yW-86, activity of EC 2.1.1.228
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S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
imG-14
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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?
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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?
S-adenosyl-L-methionine + 7-[(3S)-(3-amino-3-carboxypropyl)]-4-demethylwyosine37 in tRNAPhe
S-adenosyl-L-homocysteine + 7-[(3S)-(3-amino-3-carboxypropyl)]wyosine37 in tRNAPhe
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Nanoarchaeum equitans NEQ228 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant
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additional information
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bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)
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additional information
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bifunctional Trm5a from Pyrococcus abyssi (PaTrm5a) catalyses not only the methylation of N1, but also the further methylation of C7 on 4 demethylwyosine at position 37 to produce isowyosine (EC 2.1.1.228 and EC 2.1.1.282, respectively)
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additional information
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Pyrococcus abyssi PAB2272 protein displays a dual tRNAPhe:m1G/imG2 methyltransferase activity. Two different types of substrates are used: (1) bulk tRNA, isolated from Salmonella enterica trmDELTA27 mutant containing the unmodified G37 nucleotide leading to the formation of pm1G, and (2) tRNA, which is isolated from the Saccharomes cerevisiae DELTAtyw2 mutant that contains the imG-14 wyosine derivative leading to formation of pimG2pA dinucleotide and to a lesser extent to pm1G, likely resulting from the small amounts of G37-containing tRNAPhe present in the bulk tRNA isolates from the Scetyw2 mutant
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additional information
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structural basis for substrate recognition, the D1 domain of the enzyme undergoes large conformational changes upon the binding of tRNA., the enzyme recognizes the overall shape of tRNA. PaTrm5a adopts distinct open conformations before and after the binding of tRNA. Enzyme-substrate interactions in the catalytic domain. The anticodon interactions mostly concentrate on the A36-G37-A38 triplet. Proposed reaction mechanism of Trm5a with modified yeast tRNAPhe, overview
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additional information
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substrate-binding modes of PaTrm5a, and recognition of substrate analogues, overview
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additional information
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Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant
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additional information
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Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant
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additional information
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Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant
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additional information
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Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant
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additional information
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Sulfolobus solfataricus SSO2439 protein exhibits a tRNAPhe:imG2, but not tRNAPhe:m1G (EC 2.1.1.228), methyltransferase activity. The enzyme SSO2439 shows incorporation of the [methyl-14C] group into bulk yeast tRNA isolated from Saccharomyces cerevisiae DELTAtyw2 (containing imG-14 in tRNAPhe), but not into that from the Salmonella enterica trmDELTA27 (containing G37) mutant
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