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2.1.1.295: 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase

This is an abbreviated version!
For detailed information about 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.295

Reaction

S-adenosyl-L-methionine
+
2-methyl-6-all-trans-nonaprenylbenzene-1,4-diol
=
S-adenosyl-L-homocysteine
 +
plastoquinol

Synonyms

2-methyl-6-phytyl-1, 4-benzoquinol methyltransferase, 2-methyl-6-phytyl-1,4- benzoquinol methyltransferase, 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase, 2-methyl-6-phytylbenzoquinone methyltransferase, 2-methyl-6-solanyl-1,4-hydroquinone methyltransferase, LSMT, MPBQ methyltransferase, MPBQ MT, MPBQ-MT, MPBQ/MSBQ methyltransferase, MPBQ/MSBQ MT, MPBQ/MSBQ transferase, MT-1, MT-2, SLL0418 protein, VTE3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.295 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase

Cloned

Cloned on EC 2.1.1.295 - 2-methyl-6-phytyl-1,4-hydroquinone methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, the coding region is 1014bp long, divided into 3 exons by 2 introns, sequence comparisons, recombinant overexpression of the enzyme in Lactuca sativa leaves by Agrobacterium-mediated transformation using the pCAMBIA 2300 vector, quantitative PCR expression analysis showing that the transcription level of LsMT in transgenic plants increases by 1.5-5folds compared to that in wild-type plants. The transformants contain a 60%-93% higher level of gamma-tocopherol and 41%-93% higher level of alpha-tocopherol compred to wild-type. Overexpression of LsMT causes an elevation of plastoquinone level in lettuce
expressed in Escherichia coli
-
gene CtMPBQ MT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR and RACE expression analysis
gene VTE3, cloning of full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding the enzyme MPBQ MT, and of the full-length DNA of VTE3 (des­ignated gVTE3-1 and -2), DNA and amino acid sequence determination and analysis, sequence comparisons, and polymorphism analysis, phylogenetic analysis
gene VTE3, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons, and polymorphism analysis, phylogenetic analysis