2.1.1.299: protein N-terminal monomethyltransferase
This is an abbreviated version!
For detailed information about protein N-terminal monomethyltransferase, go to the full flat file.
Word Map on EC 2.1.1.299
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2.1.1.299
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methyltransferases
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trimethylation
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monomethylated
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trimethylase
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priming
- 2.1.1.299
- methyltransferases
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trimethylation
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monomethylated
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trimethylase
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priming
Reaction
Synonyms
alpha N-terminal protein methyltransferase 1B, alpha-N-terminal methyltransferase 2, METTL11B, N-terminal methyltransferase, N-terminal monomethylase, N-terminal RCC1 methyltransferase 2, NRMT2, NTMT2
ECTree
2.1.1.299 protein N-terminal monomethyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.1.1.299 - protein N-terminal monomethyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + PPKRIA
2 S-adenosyl-L-homocysteine + ?
mono- and dimethylation by NTMT2
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?
3 S-adenosyl-L-methionine + GPKRIA
3 S-adenosyl-L-homocysteine + ?
mono-, di-, and trimethylation by NTMT2
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-
?
N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine
N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine
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?
S-adenosyl-L-methionine + APKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + CPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + FPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + HPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + KPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2, very low activity
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?
S-adenosyl-L-methionine + LPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2, very low activity
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?
S-adenosyl-L-methionine + MPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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?
S-adenosyl-L-methionine + N-terminal-(A,P,S)PK-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,P,S)PK-[protein]
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?
S-adenosyl-L-methionine + N-terminal-Ala-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-Ala-Pro-Lys-[protein]
S-adenosyl-L-methionine + N-terminal-Pro-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Pro-Pro-Lys-[protein]
S-adenosyl-L-methionine + N-terminal-Ser-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Ser-Pro-Lys-[protein]
S-adenosyl-L-methionine + RPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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?
S-adenosyl-L-methionine + SPKRIA
S-adenosyl-L-homocysteine + ?
binding structure of SAM/SAH and of the substrate peptide, overview. SPKRIA peptide is derived from N-terminus of RCC1 protein. NTMT2, unlike NTMT1 (EC 2.1.1.244), fails to di-/tri-methylate SPKRIA
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?
S-adenosyl-L-methionine + SPKRIAKRRSPPADA
?
substrate peptide consisting of the first 15 amino acids of RCC1
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?
S-adenosyl-L-methionine + WPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + YPKRIA
S-adenosyl-L-homocysteine + ?
monomethylation by NTMT2
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-
?
S-adenosyl-L-methionine + N-terminal-Ala-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-Ala-Pro-Lys-[protein]
the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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-
?
S-adenosyl-L-methionine + N-terminal-Ala-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-Ala-Pro-Lys-[protein]
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the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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?
S-adenosyl-L-methionine + N-terminal-Pro-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Pro-Pro-Lys-[protein]
the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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?
S-adenosyl-L-methionine + N-terminal-Pro-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Pro-Pro-Lys-[protein]
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the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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-
?
S-adenosyl-L-methionine + N-terminal-Ser-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Ser-Pro-Lys-[protein]
the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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-
?
S-adenosyl-L-methionine + N-terminal-Ser-Pro-Lys-[protein]
S-adenosyl-L-homocysteine + N-terminal-N-methyl-Ser-Pro-Lys-[protein]
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the enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. In contrast to EC 2.1.1.244, protein N-terminal methyltransferase, the protein only adds one methyl group to the N-terminal
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-
?
additional information
?
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NRMT2 exhibits monomethylase activity in vitro. Isozymes NRMT1 and NRMT2 can interact both in vitro and in vivo, modeling of NRMT1 and NRMT2 interactions. No activity with the MYL9 peptide SSKRAKAKTTKKRP
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additional information
?
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NTMT2 is a mono-methyltransferase, substrate specificity, overview. But NTMT2 is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide, otherwise it is predominantly a mono-methyltransferase. The residue N89 of NTMT2 serves as a gatekeeper residue that regulates the binding of unmethylated versus mono-methylated substrate peptide.The substrate peptide is deeply inserted into the binding pocket through hydrophilic and hydrophobic interactions, and the alpha-amine of the first residue (S1) points towards the sulfur atom of SAH. In the NTMT2 complex, S1 fits snugly into the channel, where the main-chain O atom is anchored by N223 in NTMT2 (N168 in NTMT1, EC 2.1.1.244) through hydrogen bonding. No activity with peptides TPKRIA, VPKRIA, IPKRIA, DPKRIA, EPKRIA, NPKRIA, and QPKRIA
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additional information
?
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NTMT2 is a mono-methyltransferase, substrate specificity, overview. But NTMT2 is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide, otherwise it is predominantly a mono-methyltransferase. The residue N89 of NTMT2 serves as a gatekeeper residue that regulates the binding of unmethylated versus mono-methylated substrate peptide.The substrate peptide is deeply inserted into the binding pocket through hydrophilic and hydrophobic interactions, and the alpha-amine of the first residue (S1) points towards the sulfur atom of SAH. In the NTMT2 complex, S1 fits snugly into the channel, where the main-chain O atom is anchored by N223 in NTMT2 (N168 in NTMT1, EC 2.1.1.244) through hydrogen bonding. No activity with peptides TPKRIA, VPKRIA, IPKRIA, DPKRIA, EPKRIA, NPKRIA, and QPKRIA
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additional information
?
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the substrate recognition motif is X-P-K/R. Although NTMT2 is proposed to be a monomethylase, it can also fully methylate both GPKRIA and PPKRIA peptides, but to a low level. NTMT2 is able to methylate hexamer peptides
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