2.1.1.310: 25S rRNA (cytosine2870-C5)-methyltransferase
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For detailed information about 25S rRNA (cytosine2870-C5)-methyltransferase, go to the full flat file.
Reaction
Synonyms
m5C-methyltransferase, NOP2, NSUN1/Nol1, p120, proliferation-associated nucleolar antigen, protein p120, RNA:m5C-MTase
ECTree
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Cloned
Cloned on EC 2.1.1.310 - 25S rRNA (cytosine2870-C5)-methyltransferase
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cloning from genomic DNA, expression in a Saccharomyces cerevisiae Nop2 enzyme mutant and partial complementation. Full-length human p120 when expressed in Saccharomyces cerevisiae strongly accumulates in the nucleolus
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cloning from genomic DNA, recombinant expression of His-tagged full-length Nop2 and Nop2(1-220) N-terminal domain in Escherichia coli strain BL21(DE3) CodonPlus
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