2.1.1.35: tRNA (uracil54-C5)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (uracil54-C5)-methyltransferase, go to the full flat file.

Reaction

Synonyms

5-methyluridine 54 tRNA methyltransferase, m5U 54 tRNA methyltransferase, m5U 54 tRNA MTase, m5U methyltransferase, m5U-methyltransferase, methyltransferase, transfer ribonucleate uracil 5-, PAB0719, PAB0719 enzyme, PabTrmU54, ribothymidyl synthase, rTase, RumA, RumA-type tRNA(uracil-54, C5)-methyltransferase, RUMT, S-adenosyl-L-methionine-dependent tRNA (uracil-54, C5)-methyltransferase, transfer RNA uracil 5-methyltransferase, transfer RNA uracil methylase, TrmA, TRMT2A, tRNA (uracil-54, C5)-methyltransferase, tRNA uracil 5-methyltransferase, tRNA(m5U54)methyltransferase, tRNA(uracil-5-)methyltransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.35 tRNA (uracil54-C5)-methyltransferase

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Results	in table
9	Activating Compound
8744	AA Sequence
3	Application
1	CAS Registry Number
12	Cloned(Commentary)
30	Cofactor
2	Crystallization (Commentary)
19	Disease/ Diagnostics
1	General Information
2	General Stability
42	Inhibitors
4	Ki Value [mM]
23	KM Value [mM]
9	Localization
12	Metals/Ions
13	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
102	Organism
2	PDB ID
10	pH Optimum
1	pH Range
13	Protein Variants
18	Purification (Commentary)
16	Reaction
3	Reaction Type
45	Reference
2	Source Tissue
8	Specific Activity [micromol/min/mg]
6	Storage Stability
152	Substrates and Products (Substrate)
12	Subunits
26	Synonyms
1	Systematic Name
20	Temperature Optimum [°C]
1	Temperature Stability [°C]
10	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.1.1.35 - tRNA (uracil54-C5)-methyltransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-70°C, at least 6 months, stable

Escherichia coli

-

441359, 441360

-85°C, several months, stable

Escherichia coli

-

441362

0°C, partially purified, 48 h, about 30% loss of activity

Escherichia coli

-

441338

0°C, purified, 6 months, stable

Escherichia coli

-

441338

4°C, crude extract, 10 mM potassium phosphate, pH 7, 1 mM EDTA, 0.1 mM dithiothreitol, 10% v/v ethylene glycol, 0.02% sodium azide, 7 days, 10% loss of activity

Escherichia coli

-

441365