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2.1.1.4: acetylserotonin O-methyltransferase

This is an abbreviated version!
For detailed information about acetylserotonin O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.4

Reaction

S-adenosyl-L-methionine
+
N-acetylserotonin
=
S-adenosyl-L-homocysteine
 +
Melatonin

Synonyms

acetylserotonin methyltransferase, ASMT, ASMT1, ASMT2, COMT, HIOMT, hydroxindole-O-methyltransferase, hydroxyindole methyltransferase, hydroxyindole O-methyltransferase, hydroxyindole-O-methyl transferase, hydroxyindole-O-methyltransferase, MANES_17G050500, methyltransferase, acetylserotonin, N-acetylserotonin methyltransferase, N-acetylserotonin methyltransferase1, N-acetylserotonin O-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.4 acetylserotonin O-methyltransferase

Cloned

Cloned on EC 2.1.1.4 - acetylserotonin O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293 cells
-
expression analysis in eggs
-
FreeStyle 293 expression system used for cellular expression, His-tagged protein synthesized in the Rapid translation system RTS500
gene cloning, DNA and amino acid sequence determination and analysis, the At4g35160 gene exhibits the highest sequence identity (31%) to the rice ASMT gene, followed by the At1g76790 gene with 29% sequence identity, phylogenetic analysis. The recombinant proteins are recombinantly expressed from the two Arabidopsis genes. The At4g35160 recombinant protein exhibits ASMT enzyme activity, but the At1g76790 recombinant protein does not. Recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene comt, recombinant expression in Escherichia coli strain BL21(DE3), the N-terminal His-tagged AtCOMT is more highly expressed in soluble form than the C-terminal His-tagged AtCOMT
gene OsASMT, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged and of nontagged enzyme in Escherichia coli strain BL21(DE3), expression of N- or C-terminally His-tagged enzyme is not successful
-
gene OsCOMT, recombinant His6-tagged enzyme expression in Escherichia coli strain BL21(DE3), generation of transgenic Nicotiana benthamiana plants and Oryza sativa plants expressing the enzyme using the Agrobacterium tumefaciens strains GV2260 and LBA4404 transfection method
gene sequenced
-