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2.1.1.45: thymidylate synthase

This is an abbreviated version!
For detailed information about thymidylate synthase, go to the full flat file.

Word Map on EC 2.1.1.45

Reaction

5,10-methylenetetrahydrofolate
+
dUMP
=
dihydrofolate
+
dTMP

Synonyms

BgDHFR-TS, bifunctional TS-DHFR, DFR-TS, DHFR-TS, DHFR–TS, dihydrofolate reductase-thymidylate synthase, dTMP synthase, HTS, human TS, LBRM_06_0830, methylenetetrahydrofolate:dUMP C-methyltransferase, More, Thy1, ThyA, thymidylate synthase, thymidylate synthase A, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase, ThyX, TMP synthetase, TMPS, TS, TS-DHFR, TSase, TYMS, Y110A7A.4

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase

Cloned

Cloned on EC 2.1.1.45 - thymidylate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 different thymidilate synthases, bsTS-A and bsTS-B
bacterial expression vector for overproduction in Escherichia coli
-
cDNA of TS gene subcloned into T7 polymerase vector pET15-b, production of active TS by complementation of Escherichia coli
cDNAs from Thymitaq resistant clones, gene amplification with plasmid pET-17xb, expressed in Escherichia coli BL21(DE3)
-
clone harbors the pO2CLSA-4 plasmid expressed in an Escherichia coli Rue 10 expression vector
-
cloned and overexpressed in Escherichia coli
-
contains 2 genes for thymidilate synthase, ThyA and ThyB
-
Escherichia coli cells carrying an expression plasmid with the malarial dihydrofolate reductase-thymidylate synthase
-
Escherichia coli chi2913 transformed with pKPTSd containing the complete coding sequence for Lactobacillus casei TS
-
Escherichia coli NM522 cells used for cloning and library construction, Escherichia coli chi2913recA lacking TS used for expression by complementation
Escherichia coli transformed with a high amplifying expression plasmid containing the Escherichia coli thymidylate synthase gene
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 strain DE3pLysS
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells
expressed in Escherichia coli NovaBlue cells
-
expressed in Escherichia coli strain chi2913
-
expressed in Escherichia coli strain K562
-
expressed in Escherichia coli strain TX61
expressed in Escherichia coli strain TX61- cells and in the RJK88.13 cell line (an enzyme-deficient derivative of V79 Chinese hamster lung cells)
-
expressed in RJK88.13 cells and in HEp2/500 cells
-
expression in Escherichia coli
expression in Escherichia coli as fusion protein with glutathione S-transferase
expression in Escherichia coli BL21 (DE3) as a fusion protein with glutathione S-transferase (GST)
expression vector pETSM, cloned and expressed in Escherichia coli BL21(DE3)pLysS
-
histidine-tagged Mycobacterium tuberculosis ThyA is overexpressed from BL21-DE3 pLysS Escherichia coli
mutant P254D cDNA cloned into SacII/XhoI sites of the HaMSV retroviral vector and transfected into the TS negative cell line RJK88.13
-
overexpression of the encoded gene by transforming Escherichia coli Chi2913
-
plasmid containing the mutation was transformed into the Escherichia coli TX61-(thyA-) bacterial strain that does not produce its own thymidylate synthase enzyme
plasmid pSCTS9, synthetic TS gene in pUC18
-
recombinant bifunctional enzyme DHFR-TS, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain M15(pREP4)
recombinant expression of bifunctional Trypanosoma brucei DHFR-TS in form of a fusion protein incorporating Escherichia coli elongation factor Ts (Tsf), in Escherichia coli strain BL21 Star (DE3). The Tsf open reading frame is amplified by PCR from the genomic DNA of Eschrichia coli strain K12, cloning procedure overview. Expression constructs carrying a thymidylate synthase (TS) domain from Trypanosoma brucei, Leishmania major and Homo sapiens are expressed in a TS-deficient Escherichia coli strain (thyA-), while TbDHFR without thymidylate synthase is expressed in the parental thyA+ strain
synthetic DNA for TS pSCTS9 transfromed in Escherichia coli x2913recA
-
T4 thymidilate synthase gene in amplification plasmid pKC30
Tequatrovirus T4
-
thyA gene cloned, sequenced and expressed in Escherichia coli KLC1597, transformation with pBr322
-