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2.1.1.67: thiopurine S-methyltransferase

This is an abbreviated version!
For detailed information about thiopurine S-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.67

Reaction

S-adenosyl-L-methionine
+
a thiopurine
=
S-adenosyl-L-homocysteine
 +
a thiopurine S-methylether

Synonyms

6-thiopurine S-methyltransferase, 6-thiopurine transmethylase, mercaptopurine methyltransferase, thiopurine methyl transferase, thiopurine methyltransferase, thiopurine S-methyltransferase, thiopurine-S-methyl-transferase, TMPT, TPMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.67 thiopurine S-methyltransferase

Cloned

Cloned on EC 2.1.1.67 - thiopurine S-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 8.7 kb fragment encompassing part of exon III through a portion of intron 6 is subcloned into the pZERO-2 vector
-
baculovirus expressed
-
cDNA cloned and heterologous expressed in rabbit reticulocytes and wheat germ lysate
cDNA cloning, heterologous expression in yeast
-
enhanced green fluorescent protein-tagged enzyme is expressed in Escherichia coli, Jurkat, Hep-G2, and HEK-293 cells
-
expressed in COS-1 cells
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
for cloning of the TPMT fragment the TOPO TA cloning kit is used
-
into the pCR2.1-TOPO vector, used as a template to generate point mutations, the variants are sequenced and cloned into the mammalian expression vector pCMV6-XL5, into the pENTR/D-TOPO vector and subsequently into the pcDNA-DEST40 vector
-
into the yeast expression vector pYeDP60 for transformation of Saccharomyces cerevisiae cells
retroviral gene transfer
-
the full-length coding region is amplified by PCR from a eukaryotic expression plasmid and cloned into the pET-28a vector for expression in Escherichia coli BL21DE3 cells
to assess the functional effect of the trinucleotide repeat variants in the promoter, the wild-type and variant TPMT promoters are amplified and cloned into the promoter-less pGL3-Basic vector
-