2.1.1.68: caffeate O-methyltransferase

This is an abbreviated version!
For detailed information about caffeate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.68

2.1.1.68

phenylpropanoids

monolignols

syringyl

cinnamyl

guaiacyl

lignification

coniferyl

ccoaomts

caffeoyl-coa

5-hydroxylase

5-hydroxyconiferyl

n-acetylserotonin

sinapyl

s-lignin

brown-midrib

5-hydroxyconiferaldehyde

caffeoyl-coenzyme

sclerenchyma

synthesis

Reaction

Synonyms

3,4-dihydroxybenzaldehyde-O-methyltransferase, AtCOMT1, AtOMT1, benzenoid/phenylpropanoid meta/para-O-methyltransferase, Bmr12, Bradi3g16530, brown midrib 12, caffeate 3-O-methyltransferase, caffeate methyltransferase, caffeate/5-hydroxyferulate 3/5-O-methyltransferase, caffeic acid 3-O-methyltransferase, caffeic acid O-methyl-transferase, caffeic acid O-methyltransferase, caffeic acid O-methyltransferase 1, caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase, caffeic O-methyl transferase, CCOMT, COMT, COMT-3D, COMT1, COMT2, COMT4, COMT6, DOMT, FGCOMT1, Lp OMT1, O-methyltransferase, O-methytransferase, OMT, OMT1, OMT3, OsCOMT1, RsOMT1, RsOMT3, S-adenosyl-L-methionine:caffeic acid-O-methyltransferase, Sb07g003860, Van OMT-2, Van OMT-3

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.68 caffeate O-methyltransferase

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Results	in table
2	Activating Compound
126	AA Sequence
5	Application
1	CAS Registry Number
28	Cloned(Commentary)
2	Cofactor
6	Crystallization (Commentary)
6	Expression
17	General Information
4	General Stability
26	Inhibitors
10	kcat/KM [mM/s]
7	Ki Value [mM]
129	KM Value [mM]
7	Localization
2	Metals/Ions
21	Molecular Weight [Da]
26	Natural Substrates/ Products (Substrates)
66	Organism
13	Pathway
12	PDB ID
9	pH Optimum
5	pH Range
3	pI Value
37	Protein Variants
17	Purification (Commentary)
2	Reaction
1	Reaction Type
62	Reference
63	Source Tissue
23	Specific Activity [micromol/min/mg]
7	Storage Stability
164	Substrates and Products (Substrate)
9	Subunits
90	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
1	Temperature Stability [°C]
14	Turnover Number [1/s]

Purification

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Purification on EC 2.1.1.68 - caffeate O-methyltransferase

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4 entries

affinity chromatography

Medicago sativa

485542

affinity chromatography on S-adenosyl-homocysteine-agarose

Populus tremuloides

485538

by His-Bind column chromatography

Oryza sativa

675589

by nickel affinity chromatography

Vanilla planifolia

Q27I62, Q27I63

676547

HisTrap column chromatography

Arabidopsis thaliana

Q9FK25

756621

native enzyme partially by adenosine-agarose affinity chromatography, 18.2fold for 3,4-dihydroxybenzaldehyde-O-methyltransferase activity and 16.7fold for caffeic acid O-methyltransferase activity

Vanilla planifolia

Q6Q796

718207

Ni-NTA column chromatography

2 entries

Ni-NTA column chromatography and Superdex S200 gel filtration

Oryza sativa

Q6ZD89

757324

Ni2+-agarose affinity column chromatography, gel filtration

Populus trichocarpa

A9P830, C6FFB6

701663

three caffeic acid methylating activities: isozymes I, II, III, with an enzyme density of 1.277 kg/l, determined by isopycnic equilibrium centrifugation

Nicotiana tabacum

485543

using affinity chromatography

Arabidopsis thaliana

720616

using Ni-NTA chromatography

Arabidopsis thaliana

720797