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2.1.1.72: site-specific DNA-methyltransferase (adenine-specific)

This is an abbreviated version!
For detailed information about site-specific DNA-methyltransferase (adenine-specific), go to the full flat file.

Word Map on EC 2.1.1.72

Reaction

S-adenosyl-L-methionine
+
adenine in DNA
=
S-adenosyl-L-homocysteine
 +
N6-methyladenine in DNA

Synonyms

6mA DNA methyltransferases, A1S_0222, AamA, acinetobacter adenine methyltransferase A, adenine methyltransferase, adenine methyltransferase 1, adenine N6-methyltransferase, adenine-(N6)-DNA methyltransferase, adenine-N6 DNA methyltransferase, adenine-N6 MTAse, AhdI methyltransferase, AMT1, ApyPI, AquII, AquIII, AquIV, BamHI MTase, BsbI, C18A3.1, CcrM, CcrM DNA adenine methyltransferase, CcrM methylase, CdpI, cell cycle-regulated DNA methyltransferase, cell cycle-regulated methyltransferase, cell-cycle regulating MTase, CstMI, Dam, Dam DNA-(adenine-N6)-methyltransferase, Dam DNA-(adenine-N6)-MTase, Dam methylase, Dam MTase, DAMT-1, DANN 6mA MTase, DNA 6mA methyltransferase, DNA adenine 5'-GATC-3' methylase, DNA adenine methylase, DNA adenine methyltransferase, DNA adenine methyltransferases, DNA adenine MTase, DNA adenine N6-methyltransferase, DNA adenine-N6 MTase, DNA methyltransferase, DNA MTase, DNA N-6 adenine methyltransferase, DNA N6 adenine methyltransferase 1, DNA N6-adenine methyltransferase, DNA [amino]-methyltransferase, DNA-(adenine N6)-methyltransferase, DNA-(adenine-N6)-methyltransferase, DNA-(adenine-N6-)-methyltransferase, DNA-(N6-adenine)-methyltransferase, DNA-adenine methyltransferase, DNA-[adenine] methyltransferase, DNA-[adenine] MTase, DNA-[N6-adenine] MTase, DNA-[N6-adenine]-methyltransferase, DNA:m6A MTase, DNAm6A MTase, DraRI, DrdIV, EcoDam, EcoDam DNA-[N6-adenine] MTase, EcoKDam, EcoP15I, EcoP15I MTase, EcoRII DNA methyltransferase, EcoRV, EcoVIII, gamma-adenine MTase, Hia5 protein, Hin1523 protein, HP0050 methyltransferase, HP0593 DNA-(N6-adenine)-methyltransferase, HP0593 MTase, HpyAXII, HpyIIIM, isospecific adenine DNA methyltransferase, isospecific DNA MTase, KpnI DNA methyltransferase, KpnI DNA-(N6-adenine)-methyltransferase, KpnI MTase, M-RsrI, M. TthP, M.Aba17978ORF8565P, M.AbaBGORF222P, M.AluBI, M.BseCI, M.BstZ1II, M.Csp231I, M.DpnM, M.EcaI, M.EcoKCcrM, M.EcoP15I, M.EcoRI, M.EcoRII, M.EcoRV, M.EcoVIII, M.EfaBMDam, M.HindIII, M.HpyAXII, M.KpnI, M.LlaCI, M.MboIIA, M.NgoAXP, M.RsrI, M.TaqI, M1.HpyAVI, M1.MboII, M2.BstSEI, m6A methyltransferase, MaqI, MettL3-MettL14 complex, MmeI, modification methylase, More, MTA1, MTA1c, MTase, N-6 adenine-specific DNA methyltransferase 1, N6 adenine methyltransferase, N6 adenine MTase, N6-Ade MTase, N6-adenine DNA -methyltransferase, N6-adenine methyltransferase, N6-adenine MTase, N6AMT1, N6_N4_MTase, NgoAXPMod subunit, NhaXI, NlaCI, Nme1821 protein, NmeAIII, Pho(M98A)Dam, PlaDI, PspOMII, PspPRI, RceI, restriction-modification system, RpaB5I, RsrI N6-adenine DNA methyltransferase, SdeAI, sequence-specific DNA adenine methyltransferase, specific methyltransferase, SpoDI, T4 Dam, T4 Dam (N6-Ade)-MTase, T4 Dam DNA methyltransferase, T4 Dam DNA-(N6-adenine)-methyltransferase, T4 Dam MTase, T4 DNA-adenine methyltransferase, T4Dam, T4Dam DNA-[N6-adenine] MTase, T4DNA-(N6-adenine)-methyltransferase, type IC M.EcoR124I DNA methyltransferase, VspI methyltransferase, Wadmtase, [N6-adenine] MTase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.72 site-specific DNA-methyltransferase (adenine-specific)

Cloned

Cloned on EC 2.1.1.72 - site-specific DNA-methyltransferase (adenine-specific)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of T2 dam gene into expression vector pJW2, creation of T2-T4 hybrid genes in expression vector pJW2
-
expressed as glutathione S-transferase fusion protein in Escherichia coli BL21 (DE3) pLysS cells
-
expressed in dam-deficient Escherichia coli strain ER2925
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli ER2566 cells
-
expressed in Escherichia coli ER2925(DE3) cells
-
expressed in Escherichia coli GM2163 cells
-
expressed in Escherichia coli HMS174 (DE3) and ER2566 cells
-
expressed in Escherichia coli HMS174 cells
expressed in Escherichia coli Hms174(DE3) cells
expressed in Escherichia coli NEB cells
-
expressed in Escherichia coli RR1 cells
-
expressed in Escherichia coli RRI cells
-
expressed in Escherichia coli strain ER2566
expressed in Escherichia coli strain ER2683
expressed in Escherichia coli XL2 Blue cells
expressed in Escherichia coli XL2-Blue cells
expressed in Escherichia coli XL2Blue cells
-
expressed in MCF-7 cells and in MCF-10A cells
-
expressed in Nicotiana tabacum
-
expressed in Sf9 insect cells
-
expression in Escherichia coli strain DH10B
-
expression of the apo-enzyme mutant L72P as His-tagged enzyme
expression of wild-type and mutant enzymes
-
from transposon Tn1549, into plasmid pEfaORF18Ks, expressed in Escherichia coli GM2163 cells
gene ahdI, genetic organization and regulation, modelling transcription activity of the PahdICR operon from the PahdIMS promoter, expression in Escherichia coli
-
gene jhp0085, DNA sequence determination
-
gene yhdJ or ccrM, chromosomally encoded, DNA sequence determination and analysis, overvexpression decreases bacterial growth by altering cell division, cells show aberant morphology
-
genomic library construction, DNA and amino acid sequence determination and computational analysis, genetic organization, expression in Escherichia coli
His-tagged EcoDam expressed in HMS174(DE3) cells
in a T7 promoter-based vector system using Escherichia coli ER2566 as a host strain, which can alter the virulence potential
-
into a pQE60 vector containing a C-terminal 6 x His tag and transformed into Escherichia coli M15 cells
ligated into KpnI/XbaI-digested pBluescriptIIKS+, Dam overproduction in Yersinia enterocolitica, which results in an increased frequency of spontaneous mutation and marginally resistance to 2-aminopurine, subcloned into pVLT33 under the control of an inducible Ptac promoter and introduced into the dam mutant strain Escherichia coli GM2163, can complement it, overproduction in CHO cells, lleads to increased invasion of Yersinia enterocolitica
-
mutant enzymes are expressed in Escherichia coli HMS174(DE3) cells
-
mutant expressed in Escherichia coli BL21(DE3)
-
native or mutated dam gene overexpressed from the pBAD/damAhSSU pBAD/damD/A, or pBAD/damY/A plasmid under the control of an araBAD promoter, transformed to Escherichia coli GM33
-
overexpression as His-tagged protein in strain XL-2Blue
-
overproduction in Yersinia enterocolitica, increased spontaneous resistance to chloramphenicol 21.2fold and to streptomycin 39.4fold, decrease in resistance to 2-aminopurine, expression in Yersinia enterocolitica mutant strain GHY121, can complement it
-
putative Pfa MTase gene, subcloned into different expression vectors under regulatable promoters, these constructs separately transferred into Escherichia coli and yeast cells
-
subcloned into pET21b overexpression in Escherichia coli
-
the enzyme is subcloned into the plasmid expression vector pJW2
Tequatrovirus T4
-