2.1.1.72: site-specific DNA-methyltransferase (adenine-specific)

This is an abbreviated version!
For detailed information about site-specific DNA-methyltransferase (adenine-specific), go to the full flat file.

Reaction

Synonyms

6mA DNA methyltransferases, A1S_0222, AamA, acinetobacter adenine methyltransferase A, adenine methyltransferase, adenine methyltransferase 1, adenine N6-methyltransferase, adenine-(N6)-DNA methyltransferase, adenine-N6 DNA methyltransferase, adenine-N6 MTAse, AhdI methyltransferase, AMT1, ApyPI, AquII, AquIII, AquIV, BamHI MTase, BsbI, C18A3.1, CcrM, CcrM DNA adenine methyltransferase, CcrM methylase, CdpI, cell cycle-regulated DNA methyltransferase, cell cycle-regulated methyltransferase, cell-cycle regulating MTase, CstMI, Dam, Dam DNA-(adenine-N6)-methyltransferase, Dam DNA-(adenine-N6)-MTase, Dam methylase, Dam MTase, DAMT-1, DANN 6mA MTase, DNA 6mA methyltransferase, DNA adenine 5'-GATC-3' methylase, DNA adenine methylase, DNA adenine methyltransferase, DNA adenine methyltransferases, DNA adenine MTase, DNA adenine N6-methyltransferase, DNA adenine-N6 MTase, DNA methyltransferase, DNA MTase, DNA N-6 adenine methyltransferase, DNA N6 adenine methyltransferase 1, DNA N6-adenine methyltransferase, DNA [amino]-methyltransferase, DNA-(adenine N6)-methyltransferase, DNA-(adenine-N6)-methyltransferase, DNA-(adenine-N6-)-methyltransferase, DNA-(N6-adenine)-methyltransferase, DNA-adenine methyltransferase, DNA-[adenine] methyltransferase, DNA-[adenine] MTase, DNA-[N6-adenine] MTase, DNA-[N6-adenine]-methyltransferase, DNA:m6A MTase, DNAm6A MTase, DraRI, DrdIV, EcoDam, EcoDam DNA-[N6-adenine] MTase, EcoKDam, EcoP15I, EcoP15I MTase, EcoRII DNA methyltransferase, EcoRV, EcoVIII, gamma-adenine MTase, Hia5 protein, Hin1523 protein, HP0050 methyltransferase, HP0593 DNA-(N6-adenine)-methyltransferase, HP0593 MTase, HpyAXII, HpyIIIM, isospecific adenine DNA methyltransferase, isospecific DNA MTase, KpnI DNA methyltransferase, KpnI DNA-(N6-adenine)-methyltransferase, KpnI MTase, M-RsrI, M. TthP, M.Aba17978ORF8565P, M.AbaBGORF222P, M.AluBI, M.BseCI, M.BstZ1II, M.Csp231I, M.DpnM, M.EcaI, M.EcoKCcrM, M.EcoP15I, M.EcoRI, M.EcoRII, M.EcoRV, M.EcoVIII, M.EfaBMDam, M.HindIII, M.HpyAXII, M.KpnI, M.LlaCI, M.MboIIA, M.NgoAXP, M.RsrI, M.TaqI, M1.HpyAVI, M1.MboII, M2.BstSEI, m6A methyltransferase, MaqI, MettL3-MettL14 complex, MmeI, modification methylase, More, MTA1, MTA1c, MTase, N-6 adenine-specific DNA methyltransferase 1, N6 adenine methyltransferase, N6 adenine MTase, N6-Ade MTase, N6-adenine DNA -methyltransferase, N6-adenine methyltransferase, N6-adenine MTase, N6AMT1, N6_N4_MTase, NgoAXPMod subunit, NhaXI, NlaCI, Nme1821 protein, NmeAIII, Pho(M98A)Dam, PlaDI, PspOMII, PspPRI, RceI, restriction-modification system, RpaB5I, RsrI N6-adenine DNA methyltransferase, SdeAI, sequence-specific DNA adenine methyltransferase, specific methyltransferase, SpoDI, T4 Dam, T4 Dam (N6-Ade)-MTase, T4 Dam DNA methyltransferase, T4 Dam DNA-(N6-adenine)-methyltransferase, T4 Dam MTase, T4 DNA-adenine methyltransferase, T4Dam, T4Dam DNA-[N6-adenine] MTase, T4DNA-(N6-adenine)-methyltransferase, type IC M.EcoR124I DNA methyltransferase, VspI methyltransferase, Wadmtase, [N6-adenine] MTase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.72 site-specific DNA-methyltransferase (adenine-specific)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	Activating Compound
98261	AA Sequence
42	Application
1	CAS Registry Number
70	Cloned(Commentary)
14	Crystallization (Commentary)
277	Disease/ Diagnostics
63	General Information
1	IC50 Value
35	Inhibitors
27	kcat/KM [mM/s]
8	Ki Value [mM]
87	KM Value [mM]
8	Localization
7	Metals/Ions
30	Molecular Weight [Da]
181	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
257	Organism
123	PDB ID
14	pH Optimum
1	pH Range
1	pI Value
114	Protein Variants
45	Purification (Commentary)
10	Reaction
1	Reaction Type
225	Reference
56	Source Tissue
4	Specific Activity [micromol/min/mg]
2	Storage Stability
305	Substrates and Products (Substrate)
43	Subunits
486	Synonyms
10	Temperature Optimum [°C]
3	Temperature Stability [°C]
92	Turnover Number [1/s]

Substrates Products

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Substrates Products on EC 2.1.1.72 - site-specific DNA-methyltransferase (adenine-specific)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Substrate/Product Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + (CGG*CCG)73 oligodeoxynucleotide duplexes 5'-GATC/5'-GATC

?

Tequatrovirus T4

-

-

659268

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + DNA containing N6-methyladenine

Escherichia coli

-

the enzyme specifically binds double-stranded DNA

757760

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

6 entries

S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA

S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA

2 entries

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

109 entries

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

86 entries

S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

4 entries

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

6 entries

S-adenosyl-L-methionine + Int1 DNA

S-adenosyl-L-homocysteine + Int1 DNA containing N6-methyladenine

2 entries

S-adenosyl-L-methionine + lambda phage DNA

?

2 entries

S-adenosyl-L-methionine + mRNA

S-adenosyl-L-homocysteine + mRNA containing N6-methyladenine

Caulobacter vibrioides

-

-

757760

-

-

?

S-adenosyl-L-methionine + oligodeoxynucleotide duplexes

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

substrate contains one single specific recognition site as 5'-GATC/5'-GATC, or modified variants

659268

-

-

?

S-adenosyl-L-methionine + pUC19 DNA adenine

S-adenosyl-L-homocysteine + pUC19 DNA 6-methyladenine

Escherichia coli

-

-

702115

-

-

?

S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

4 entries

S-adenosyl-L-methionine + single stranded DNA adenine

S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine

2 entries

S-adenosyl-L-methionine + T7 DNA adenine

S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine

2 entries

S-adenosyl-L-methionine + T7 phage DNA

?

2 entries

additional information

?

-

64 entries

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Caenorhabditis elegans

-

-

742235

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Caulobacter vibrioides

-

-

757591

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Cellulosimicrobium cellulans

-

-

756279

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Cellulosimicrobium cellulans B

-

-

756279

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Escherichia coli

-

-

659422

-

-

?

S-adenosyl-L-methionine + adenine in DNA

S-adenosyl-L-homocysteine + N6-methyladenine in DNA

Klebsiella pneumoniae

-

-

659303

-

-

r

S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA

S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA

Escherichia phage T2

-

-

485577

-

-

?

S-adenosyl-L-methionine + adenine in T4 gt- dam- DNA

S-adenosyl-L-homocysteine + N6-methyladenine in T4 gt- dam- DNA

Tequatrovirus T4

-

-

485567, 485577

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Aeromonas hydrophila

-

-

673988, 673989, 719550

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Aeromonas hydrophila

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Aeromonas hydrophila SSU

-

-

673988, 673989

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Aggregatibacter actinomycetemcomitans

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Amoeba proteus

-

the sams1 gene is methylated at an internal adenine residue of GATC site in symbiont-bearing amoebae but not in symbiont-free amoebae, suggesting that the modification may have caused the inactivation of sams1 at the transcriptional level

705101

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Amoeba proteus D

-

the sams1 gene is methylated at an internal adenine residue of GATC site in symbiont-bearing amoebae but not in symbiont-free amoebae, suggesting that the modification may have caused the inactivation of sams1 at the transcriptional level

705101

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Bacillus sp. (in: Bacteria)

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Bacillus sp. (in: Bacteria) NEB686

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Brucella abortus

-

the DNA target sequence is GANTC

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Campylobacter jejuni

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Caulobacter vibrioides

-

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Caulobacter vibrioides

-

the DNA target sequence is GANTC, CcrM is more active on hemimethylated than unmethylated DNA

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Caulobacter vibrioides

-

the target sequence is 5'GATC3'

703347

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Caulobacter vibrioides

-

129-mer DNA or pUC19 DNA are used as substrates

720594

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Caulobacter vibrioides

P0CAW2

the enzyme methylates the adenine of hemimethylated GANTC after replication

757749

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Cereibacter sphaeroides

-

-

676217

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Cereibacter sphaeroides

P14751

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Corynebacterium diphtheriae

Q6NFX9

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Corynebacterium striatum

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Corynebacterium striatum M82B

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Deinococcus radiodurans

C0LTP9

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Deinococcus radiodurans NEB479

C0LTP9

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Deinococcus radiodurans RI

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Edwardsiella tarda

D5FM16

Dam catalyzes the methylation of N-6 of the adenine residue in GATC sequences

706888

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Edwardsiella tarda TXD1

D5FM16

Dam catalyzes the methylation of N-6 of the adenine residue in GATC sequences

706888

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Enterobacter cloacae

-

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

-

671878, 702628, 713742

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

P10484

-

676206

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

P0AEE8

-

675380

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

Dam belongs to the alpha-class of adenine methyltransferases and transfers a methyl group to the N-6 position of the adenine in the DNA sequence 5'-GATC-3'

702334

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

Dam methylates a GATC recognition site

705127

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

Dam methylates the adenine residue in GATC sites

703200

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

P0AEE9

Dam methylates the N-6 position of adenine in the DNA sequence 5'-GATC-3' and is highly processive, catalyzing multiple methyltransfers prior to dissociating from the DNA

704502

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

P0AEE8

Dam methylates the N-6 position of the adenine in the sequence 5'-GATC-3', Dam shows a dramatic preference for the in vitro methylation of certain GATC sequences in plasmids and PCR-derived DNAfragments

704430

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

DNA adenine methyltransferase methylates the N6 positions of adenines in the sequence 5'-GATC-3'

704168

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

EcoP15I MTase adds a methyl group to the second adenine in the recognition sequence 5'-CAGCAG-3' in the presence of S-adenosyl-L-methionine

702115

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the target sequence is 5'GATC3'

703347

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the wild type enzyme shows target specificity for GATC sites

705175

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the EcoRV DNA methyltransferase methylates the first adenine in the GATATC recognition sequence

719096

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the enzyme methylates adenines at the N6 position of palindromic 5’-GATC-3’ sites. The enzyme ethylates both strands of the same site prior to fully dissociating from the DNA, a process referred to as intrasite processivity. Intrasite processivity is disrupted when the DNA flanking a single GATC site is longer than 400 bp on either side. The introduction of a second GATC site within this flanking DNA reinstates intrasite methylation of both sites

720029

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Escherichia coli E1585-68

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Geobacillus stearothermophilus

-

the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates

705646

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Geobacillus stearothermophilus SE-589

-

the enzyme modifies adenine in the nickase recognition site 5'-GAGTC-3' and is specific for 5'-GASTC-3' substrates

705646

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae

-

the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA

720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae biotype aegyptius

G9C944

the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA

720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae biotype aegyptius ATCC 11116

G9C944

the Hia5 protein causes the methylation of 61% of the adenines in lambda DNA

720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae RD

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Haemophilus influenzae Rd (FluMu)

-

the Hin1523 protein causes the methylation of 29.6% of the adenines in lambda DNA

720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori

-

M.HpyAXII targets GTAC sites

694457

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori

-

duplex 2 DNA, 5'-GCAG-3' is the recognition site for the enzyme

720816

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori

-

HP0050 methyltransferase methylates one adenine at a time in the sequence 5'-GAAG-3'

719450

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori

-

the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3'

757886

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori 26695

-

the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3'

757886

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Helicobacter pylori NSH57

-

M.HpyAXII targets GTAC sites

694457

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Homo sapiens

-

the enzyme complex is active on single-stranded and unpaired DNA in vitro and methylates GGACT in single-strand DNA and double-strand DNA-containing mismatches

756389

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Klebsiella pneumoniae

-

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Klebsiella pneumoniae

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Lactococcus cremoris

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Lactococcus cremoris W15

-

the enzyme recognizes the palindromic specific sequence 5'-AAGCTT-3' and catalyzes formation of N6-methyladenine at the first A-residue. The enzyme prefers DNA containing a hemimethylated target site

758441

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Marinobacter nauticus

A1U7P0

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Methylophilus methylotrophus

B2MU09

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Methylophilus methylotrophus

B2MU09

MmeI modifies only the adenine in the top strand, 5’-TCCRAC-3’, MmeI endonuclease activity is blocked by this top strand adenine methylation and is unaffected by methylation of the adenine in the complementary strand, 5’-GTYGGA-3'

694455

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Moraxella bovis

P23192

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Moraxella bovis

-

M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine

705556

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria gonorrhoeae

-

the specific sequence recognized by M.NgoAXP is 5'-CCACC-3', in which the adenine residue is methylated

703759

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria gonorrhoeae FA 1090

-

the specific sequence recognized by M.NgoAXP is 5'-CCACC-3', in which the adenine residue is methylated

703759

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria lactamica

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria lactamica ST640 Sange

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria meningitidis

-

-

705971, 720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria meningitidis Z2491

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Neisseria meningitidis Z2491 (Pnme1)

-

-

720595

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Nitrobacter hamburgensis

Q1QGU9

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Nitrobacter hamburgensis X14

Q1QGU9

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Oxytricha trifallax

-

the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA

756367

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Oxytricha trifallax JRB310

-

the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA

756367

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Parvibaculum lavamentivorans

A7HWD2

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Pasteurella multocida

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Photorhabdus luminescens

-

-

756790

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Photorhabdus luminescens TT01

-

-

756790

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Pseudomonas sp.

C0LTP8

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Pseudomonas sp. OM2164

C0LTP8

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Psychrobacter sp.

A5WI42

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Rhodopseudomonas palustris

Q134M6

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Rhodopseudomonas palustris BisB5

Q134M6

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Rhodospirillum centenum

B6IW55

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Rhodospirillum centenum SW

B6IW55

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Ruegeria pomeroyi

Q5LS45

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Salmonella enterica

-

-

703875

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Salmonella enterica

-

Dam catalyzes postreplicative methylation of adenosine moieties located in 5'-GATC-3' sites

704282

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Salmonella enterica

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Salmonella enterica

-

the target sequence is 5'GATC3'

703347

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Streptococcus pneumoniae

P04043

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Sulfurimonas denitrificans

Q30TC2

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Synechococcus sp. PCC 7002

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Synechococcus sp. PCC 7002 PR-6

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Synechocystis sp.

-

recognition sequence GATC

705744

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Tequatrovirus T4

P04392

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Tetrahymena thermophila

-

-

672294, 757856

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Tetrahymena thermophila

-

the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA

756367

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Tetrahymena thermophila SB210

-

the enzyme consists of two MT-A70 proteins and two homeobox-like DNA-binding proteins and specifically methylates ApT dinucleotides in double stranded DNA. The enzyme activity is 3-3.5fold higher on hemimethylated substrates, relative to unmethylated double stranded DNA

756367

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Tetrahymena thermophila SB210

-

-

757856

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Thermus aquaticus

P14385

-

671878

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Trueperella pyogenes

-

-

705971

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Vibrio cholerae serotype O1

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Yersinia enterocolitica

-

the DNA target sequence is GATC, the natural substrate for the enzyme is hemimethylated DNA, where one strand is methylated and the other is not

703765

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methyladenine

Yersinia pseudotuberculosis

-

the target sequence is 5'GATC3'

703347

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Aliivibrio fischeri

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Bacillus amyloliquefaciens

-

-

659200

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Bacteria

-

-

485564

-

485564

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Caulobacter vibrioides

-

the enzyme preferentially binds DNA before S-adenosylmethionine. S-Adenosylhomocysteine is preferentially released from the enzyme before fully methylated DNA. Binding of both substrates and methylation occurs first in a rapid step followed by regeneration of enzyme in a second rate-limiting step

485571

-

485571

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Caulobacter vibrioides

-

the enzyme is involved in cell cycle regulation of Caulobacter crescentus

485571

-

485571

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Caulobacter vibrioides

-

a biotinylated double stranded 23mer oligodeoxynucleotide with one hemimethylated GANTC site is used as substrate

733427

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Cereibacter sphaeroides

P14751

-

659224

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Cereibacter sphaeroides

P14751

substrate and product binding site structures

659224

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Deinococcus proteolyticus

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Deinococcus proteolyticus MRP

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

-

658407, 659715, 660031, 720604

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

P0AEE8

-

733365, 734804

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

-

734804

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

canonical 14mers and various substituted duplexes. Non-selfcomplementary tetradecamer duplex d[GCCGGATCTAGACG]*d[CGTCTAGATCCGGC] containing the hemimethylated GATC target sequence on one or the other strand and modifications in the GATC target sequence of the complementary strands. Large differences in DNA methylation of duplexes containing single dI or dG substitutions of the Dam recognition site are observed compared with the canonical substrate, if the substitution involves the top strand, on the G-C rich side. Substitution in either strand by uracil or 5-ethyluracil result in small perturbation of the methylation patterns. When 2,6-diamino-purine replaces the adenine to be methylated, small but significant methylation is observed

485578

-

485578

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

non-self-complementary tetradecanucleotide duplexes that contain the GATC target sequence. The enzyme is rather tolerant to base modification, binding of the enzyme is inversely proportional to the thermodynamic stability of the duplex in the ternary complex

485572

-

485572

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

methylation of DNA in a sequence specific manner, low substrate specificity with respect to the target base. Cytosine residues can be methylated if they are located in a C:T mismatch base pair at the target position of the enzyme, modification of cytosine residues at position N4

485579

-

485579

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

enzyme is a critical regulator of bacterial virulence

659422

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

methylation of the site GATCprox proximal to the promotor is required for transition to the phase On state by specifically blocking PapI-dependent binding of Lrp to promotor proximal sites 4-6, expression of pyelonephritis-associated pili, i.e. Pap, in uropathogenic Escherichia coli is epigenetically controlled by a reversible OFF to ON switch, overview

659920

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

the enzyme is cell cycle regulated and essential for viability

659040

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

DNA substrate from calf thymus, methylation of adjacent GATC sites is distributive with DNA derived from a genetic element that controls the transcription of the adjacent genes, the first methylation event is followed by enzyme release

659422

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

enzyme methylates the first adenine in the sequence ATGCAT

659040

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

methylation of target sequence GATC

659920

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

required substrate recognition target sequence is GATC, occuring base flipping in absence of S-adenosyl-L-methionine is a biphasic process and very fast, but binding of the flipped base into the active site pocket requiring S-adenosyl-L-methionine is slow, active site contains the conserved DPPY motif, whose tyrosine184 residue stacks to the flipped target base

659715

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

specific for methylation site sequence GATC and structure, an intact GA sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview

660031

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved are Y119, N120, L122, R124, and P134, overview

658407

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia coli

-

the enzyme recognizes 5'-GATC-3'

733356

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia phage T2

-

-

485576

-

485576

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Escherichia phage T2

-

nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does

485577

-

485577

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Hapunavirus VP882

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Helicobacter pylori

-

-

658497

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Helicobacter pylori

-

enzyme is not important in mismatch repair and for adherence of the bacterium to host cells, e.g. HEp-2 cells

658497

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Klebsiella pneumoniae

-

enzyme is a member of a restriction-modification system, R-M system, plasmid DNA, and hemi- or unmethylated duplex DNA, preference for hemimethylated DNA

659303

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Klebsiella pneumoniae

-

enzyme recognizes the sequence 5'-GGTACC-3', methylation at position N6, enzyme utilizes plasmid DNA, and hemi- or unmethylated duplex DNA, preference for hemimethylated DNA

659303

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Paludibacter propionicigenes

E4T573

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Paludibacter propionicigenes WB4T

E4T573

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Planomicrobium okeanokoites

-

-

485579

-

485579

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Pyrococcus horikoshii

-

the enzyme catalyzes the methylation of adenine at the N-6 position within its DNA recognition sequence GATC. Enzymatic methylation of the hemimethylated GATC site results in destabilisation of the duplex, increasing the proportion of dissociated DNA, and producing an observable increase in fluorescence. By using a suitable oligonucleotide substrate, labeled on adjacent strands with a fluorophore (fluorescein) and quencher (dabcyl), the destabilization of duplex DNA due to adenine methylation can be monitored by fluorescence measurements. The separation of fluorophore and quencher during strand dissociation causes an observable increase in fluorescence, providing a reproducible, direct, and real-time activity assay

744009

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

-

485576

-

485576

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

-

659301, 660031

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

the enzyme mediates methyl group transfer reaction from S-adenosyl-L-methionine to adenine in the palindromic recognition sequence, GATC, of a 20-mer oligonucleotide duplex

485569

-

485569

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

in unmethylated target (2A/A duplex), the enzyme alone randomly binds to the asymetric 2A/A duplex, S-adenosyl-L-methionine induces an allosteric T4 conformational change that promotes reorientation of the enzyme to the strand containing the native base. S-Adenosyl-L-methionine increases enzyme binding-specificity, in addition to serving as the methyl donor, the enzyme recognizes the palindromic sequence GATC

485566

-

485566

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

various synthetic oligonucleotide substrates. Upon collision of an enzyme monomer with an oligonucleotide duplex, an asymmetrical complex forms in which the enzyme (randomly oriented relative to one of the strands of the specific recognition site) catalyzes a fast transfer of the methyl group from S-adenmosylmethionine to the adenosine residue. Simultaneously, a second T4MTAse subunit is added to the complex, providing for the continuation of the reaction

485568

-

485568

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

the enzyme catalyzes methyl group transfer from S-adenosyl-L-methionine to the N6-position of adenine in the palindromic sequence GATC. The rate-limiting step is release of product methylated DNA from the enzyme

485573

-

485573

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

oligonucleotide substrates containing the native or modified recognition site. The enzyme recognizes the palindromic sequence GATC and catalyzes transfer of the methyl group from S-adenosyl-L-methionine to the N6-position of adenine. The release of product is the rate-limiting step in the reaction

485570

-

485570

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

canonical 12-mer and 20-mers various defective duplexes containing some defect in the DNA-target site, e.g. the absence of an internucleotide phosphate or a nucleotide within the recognition site, or a single-stranded region

485573

-

485573

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

nonglucosylated unmethylated T4 gt- dam- DNA. The enzyme methylates the palindromic tetranucleotide, GATC, designated the canonical sequence. At high Mtase:DNA ratios, T4 Dam can methylate some noncanonical sequences belonging to GAY, where Y represents cytosine or thymine

485567

-

485567

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

synthetic oligonucleotide substrates containing the native recognition site GATC or its modified variants

485568

-

485568

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

interaction with substrates containing defective recognition sites. Deoxyguanosine residues in both strands of the modified GATC are indispensable for complex formation

485574

-

485574

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

nonglucosylated, hydroxymethylcytosine-containing T2gt- virion DNA has a higher level of methylation than T4gt virion DNA does

485577

-

485577

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

hemimethylated DNA

659905

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

P04392

methylation of DNA-adenine at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as in bacterial virulence

659989

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

specific target sequence is GATC

658407

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

unmethylated or hemimethylated DNA

659268

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

binding site is the GATC sequence, the adenine is located in the palindromic recognition site GATC, substrate binding mechanism

659905

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

specific for methylation site sequence GATC and structure, an intact GAT sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview

660031

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

specific methylation of oligonucleotide duplexes containing one or two target sequence GATC sites

659301

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

P04392

specific methylation of target sequence GATC

659989

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

specific target sequence is GATC, transition of enzyme-DNA interaction from nonspecific to specific interaction utilizing different substrates, identification of discriminatory contacts stabilizing the transition state, and antidiscriminatory contacts not affecting the methylation of the cognate site but disfavor activity at noncognate sites, involved residues are M114, R116, P126, G128, R130, F111, S112, D171, K11, and Y174, overview, flipping of target adenine

658407

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Tequatrovirus T4

-

unmethylated or hemimethylated DNA, two distinct stages of methylation under single turnover conditions

659268

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Thermus aquaticus

-

-

734030

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Treponema succinifaciens

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Treponema succinifaciens DSM 2489

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

GGGTGATCAGGG

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

CCCTGATCACCC

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

the enzyme de novo methylates the first adenine residue in the TGATCA sequence in the single-stranded or double-stranded DNA substrate, but it prefers single-stranded structures

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

GCGTGATCACGC

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

GGGTGATCAGGG+CCCTGATCACCC, annealed

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

lambda phage DNA

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Triticum aestivum

-

the enzyme is responsible for mitochondrial DNA modification that might be involved in the regulation of replication of mitochondria in plants

485565

-

485565

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio anguillarum

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio anguillarum 775

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio campbellii

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio campbellii ATCC BAA-1116

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio cholerae

A5F520

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio cholerae O395

A5F520

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio cholerae serotype O1

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio parahaemolyticus

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio parahaemolyticus RIMD

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio phage VHML

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio sp.

-

-

485580

-

485580

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio sp.

-

the enzyme modifies the third adenine within the recognition sequence 5-ATTAAT-3'

485576

-

485576

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio splendidus

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio splendidus LGP32

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio vulnificus

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Vibrio vulnificus MO6-24/O

-

the enzyme methylates GATC sequences

734045

-

-

?

S-adenosyl-L-methionine + DNA adenine

S-adenosyl-L-homocysteine + DNA 6-methylaminopurine

Yersinia pseudotuberculosis

-

-

735330

-

-

?

S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Escherichia coli

-

100% activity

757078

-

-

-

S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Escherichia coli E1585-68

-

100% activity

757078

-

-

-

S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Lactococcus cremoris

-

100% activity

757078

-

-

-

S-adenosyl-L-methionine + DNA duplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Lactococcus cremoris W15

-

100% activity

757078

-

-

-

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Escherichia coli

-

-

758441

-

-

?

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Escherichia coli E1585-68

-

-

758441

-

-

?

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Haemophilus influenzae

-

-

758441

-

-

?

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Haemophilus influenzae RD

-

-

758441

-

-

?

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Lactococcus cremoris

-

-

758441

-

-

?

S-adenosyl-L-methionine + double stranded DNA adenine

S-adenosyl-L-homocysteine + double stranded DNA 6-methyladenine

Lactococcus cremoris W15

-

-

758441

-

-

?

S-adenosyl-L-methionine + Int1 DNA

S-adenosyl-L-homocysteine + Int1 DNA containing N6-methyladenine

Acinetobacter baumannii

-

adenine methylation occurs at a GAATTC site

758277

-

-

?

S-adenosyl-L-methionine + Int1 DNA

S-adenosyl-L-homocysteine + Int1 DNA containing N6-methyladenine

Acinetobacter baumannii ATCC 17978

-

adenine methylation occurs at a GAATTC site

758277

-

-

?

S-adenosyl-L-methionine + lambda phage DNA

?

Cellulosimicrobium cellulans

-

the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3'

756279

-

-

?

S-adenosyl-L-methionine + lambda phage DNA

?

Cellulosimicrobium cellulans B

-

the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3'

756279

-

-

?

S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Escherichia coli

-

low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated

757078

-

-

-

S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Escherichia coli E1585-68

-

low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated

757078

-

-

-

S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Lactococcus cremoris

-

low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated

757078

-

-

-

S-adenosyl-L-methionine + RNA/DNA heteroduplex carrying the 5'-AAGCUU-3'/3'-TTCGAA-5' target sequence

?

Lactococcus cremoris W15

-

low activity. Only the DNA strand of the RNA/DNA heteroduplex is methylated

757078

-

-

-

S-adenosyl-L-methionine + single stranded DNA adenine

S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine

Escherichia coli

-

the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity

758441

-

-

?

S-adenosyl-L-methionine + single stranded DNA adenine

S-adenosyl-L-homocysteine + single stranded DNA 6-methyladenine

Escherichia coli E1585-68

-

the level of single stranded DNA methylation is 7fold lower than double stranded DNA. However, upon star activity conditions (30% DMSO), the efficiency of single stranded DNA modification is raised by up to 50% of the relative activity

758441

-

-

?

S-adenosyl-L-methionine + T7 DNA adenine

S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine

Geobacillus stearothermophilus

-

-

705646

-

-

?

S-adenosyl-L-methionine + T7 DNA adenine

S-adenosyl-L-homocysteine + T7 DNA 6-methyladenine

Geobacillus stearothermophilus SE-589

-

-

705646

-

-

?

S-adenosyl-L-methionine + T7 phage DNA

?

Cellulosimicrobium cellulans

-

the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3'

756279

-

-

?

S-adenosyl-L-methionine + T7 phage DNA

?

Cellulosimicrobium cellulans B

-

the enzyme modifies the adenine residue with formation of 5'-(m6A)GCT-3'

756279

-

-

?

additional information

?

-

Aeromonas hydrophila

-

the enzyme is part of the type II restriction-modification system AhdI, overview

689253

-

-

?

additional information

?

-

Bacillus amyloliquefaciens

-

the common methyl transfer from S-adenosyl-L-methionine to an exocyclic amino group, catalyzed by many enzymes, does not dictate a common kinetic scheme for MTases, comparison to T4Dam MTase from bacteriophage T4

659200

-

-

?

additional information

?

-

Bacillus sp. (in: Bacteria)

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Bacillus sp. (in: Bacteria) NEB686

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Caulobacter vibrioides

-

the enzyme has a high specificity for GANTC sites, with only minor preferences at the central position. It slightly (1.5fold) prefers hemimethylated DNA over methylated DNA

720594

-

-

?

additional information

?

-

Caulobacter vibrioides

-

the enzyme methylates GANTC sequences but does not specifically bind to DNA containing GANTC sequences

733427

-

-

?

additional information

?

-

Caulobacter vibrioides

-

the enzyme methylates adenine residues in 5'-GANTC-3' sequences

757591

-

-

-

additional information

?

-

Caulobacter vibrioides

-

the enzyme does not specifically bind to DNA containing GANTC sequences

757760

-

-

-

additional information

?

-

Corynebacterium diphtheriae

Q6NFX9

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Corynebacterium striatum

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Corynebacterium striatum M82B

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Deinococcus radiodurans

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Deinococcus radiodurans

C0LTP9

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Deinococcus radiodurans NEB479

C0LTP9

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Deinococcus radiodurans RI

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Escherichia coli

-

substrate specificity with different oligonucleotides, substrate binding specificity, overview

659715

-

-

?

additional information

?

-

Escherichia coli

-

catalyzes the transfer of a methyl group to the C5 position of the 3'-side cytosine of each strand of the recognition sequence, M.EcoRII binding is diminished by factors of 5-30 but the catalytic activity is either abolished or reduced 4-80fold when trans-anti-B[a]P-N2-dG lesions are introduced into the EcoRII recognition sequence, methylation rates are also diminished and in some cases entirely abolished, depending on the position of the lesion within the recognition sequence

664054

-

-

?

additional information

?

-

Escherichia coli

-

DNA methylation by DAM may not globally affect gene transcription by physically blocking access of transcription factors to binding sites, Dam is down regulated in the stationary phase, which correlates with the enrichment of GATC in binding sites for CRP and Sigma 38

676746

-

-

?

additional information

?

-

Escherichia coli

-

the preferred substrate consists of the annealed product of oligonucleotides 5'-CATTTACTTGATCCGGTATGC-3' and 5'-GCATACCGGATCAAGTAAATG-3', while the nonpreferred substrate consists of the annealed product of oligonucleotides 5'-CATTTAGACGATCTTTTATGC-3' and 5'-GCATAAAAGATCGTCTAAATG-3'

702334

-

-

?

additional information

?

-

Escherichia coli

-

the wild type Dam shows no detectable activity at GATT sites

705175

-

-

?

additional information

?

-

Escherichia coli

P0AEE8

the wild type enzyme is active on hemimethylated and unmethylated DNA substrates

733365

-

-

?

additional information

?

-

Escherichia coli

P0AEE8

relative to GATC, three near-cognate substrates that carry a base-pair substitution at the first position are still methylated by the enzyme, although at rates reduced by 100fold (AATC) or 1000fold (CATC)

734804

-

-

?

additional information

?

-

Escherichia coli

-

relative to GATC, three near-cognate substrates that carry a base-pair substitution at the first position are still methylated by the enzyme, although at rates reduced by 100fold (AATC) or 1000fold (CATC)

734804

-

-

?

additional information

?

-

Escherichia coli

-

the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT

758441

-

-

-

additional information

?

-

Escherichia coli

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3')

758441

-

-

-

additional information

?

-

Escherichia coli E1585-68

-

the DNA substrate preference for M.EcoVIII is: hemimethylated AAGCTT > AAGCTT > GAGCTT >/= CAGCTT > AATCTT > ATGCTT > ACGCTT > TAGCTT > AACCTT = single stranded AAGCTT > AGGCTT = AAACTT

758441

-

-

-

additional information

?

-

Escherichia coli E1585-68

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3')

758441

-

-

-

additional information

?

-

Haemophilus influenzae

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine

758441

-

-

-

additional information

?

-

Haemophilus influenzae RD

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine

758441

-

-

-

additional information

?

-

Helicobacter pylori

-

the enzyme recognizes and methylates GAGG (100% activity, Km 0.00522 mM), GGAG (about 48% activity, Km 0.017 mM) and GAAG (about 30% activity, Km 0.013 mM), but does not methylate GTGG, GmAmAG or GAGA

719450

-

-

?

additional information

?

-

Homo sapiens

Q9Y5N5

purified recombinant enzyme, in the presence of S-adenosyl-L-methionine and other cofactors, is unable to methylate trivalent inorganic arsenic or monomethylarsonous acid

719373

-

-

?

additional information

?

-

Homo sapiens

-

purified recombinant enzyme, in the presence of S-adenosyl-L-methionine and other cofactors, is unable to methylate trivalent inorganic arsenic or monomethylarsonous acid

719373

-

-

?

additional information

?

-

Homo sapiens

-

the enzyme complex shows strong activity on single-stranded oligo 6 T (containing GGACT), much reduced activities on 4 T (containing GGAGT) and 4B (AGACT), and no activity on oligos having two substitutions within the recognition, e.g. 6B (AGAGT) or 5 T and 5B (GGATC). Furthermore, the enzyme complex has no activity on an oligo-containing G and C only and no activity on A-containing oligos without matching consensus sequence. The enzyme complex acts on single-stranded DNA (6 T) faster than that on the corresponding single-stranded RNA and is inactive on double-stranded DNA or RNA/DNA hybrid

756389

-

-

-

additional information

?

-

Lactococcus cremoris

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine

758441

-

-

-

additional information

?

-

Lactococcus cremoris W15

-

the enzyme is not able to methylate nucleotide sequence containing two mismatches (5'-CAGCTG-3') and single stranded DNA adenine

758441

-

-

-

additional information

?

-

Marinobacter nauticus

A1U7P0

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Methylophilus methylotrophus

B2MU09

MmeI does not produce any detectable N4-cytosine methylation

694455

-

-

?

additional information

?

-

Methylophilus methylotrophus

B2MU09

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Moraxella bovis

-

no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA

705556

-

-

?

additional information

?

-

Neisseria lactamica

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Neisseria lactamica ST640 Sange

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Neisseria meningitidis

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Neisseria meningitidis Z2491

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Nitrobacter hamburgensis

Q1QGU9

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Nitrobacter hamburgensis X14

Q1QGU9

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Parvibaculum lavamentivorans

A7HWD2

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Pseudomonas sp.

C0LTP8

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Pseudomonas sp. OM2164

C0LTP8

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Psychrobacter sp.

A5WI42

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Rhodopseudomonas palustris

Q134M6

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Rhodopseudomonas palustris BisB5

Q134M6

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Rhodospirillum centenum

B6IW55

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Rhodospirillum centenum SW

B6IW55

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Ruegeria pomeroyi

Q5LS45

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Salmonella enterica

-

Dam-dependent regulation of secretion of pathogenicity island 1 is transmitted via transcriptional regulator HilD

703875

-

-

?

additional information

?

-

Sulfurimonas denitrificans

Q30TC2

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Synechococcus sp. PCC 7002

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Synechococcus sp. PCC 7002 PR-6

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?

additional information

?

-

Synechocystis sp.

-

plastid transcription is largely insensitive to adenine methylation of the plastid DNA

705744

-

-

?

additional information

?

-

Tequatrovirus T4

-

S-adenosyl-L-methionine plays a crucial role in reorientation of the enzyme in DNA with mutationally altered Ade residues to 2-aminopurine, overview

659301

-

-

?

additional information

?

-

Tequatrovirus T4

-

DNA substrate specificities of wild-type and mutant enzymes

658407

-

-

?

additional information

?

-

Tequatrovirus T4

-

enzyme and S-adenosyl-L-methionine form a catalytically active complex

659268

-

-

?

additional information

?

-

Tequatrovirus T4

P04392

enzyme binds also nonspecific to DNA, linear diffusion along the DNA, overview

659989

-

-

?

additional information

?

-

Trueperella pyogenes

-

the enzyme does not produce any detectable N4-cytosine methylation

705971

-

-

?