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2.1.1.80: protein-glutamate O-methyltransferase

This is an abbreviated version!
For detailed information about protein-glutamate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.80

Reaction

S-adenosyl-L-methionine
+
protein L-glutamate
=
S-adenosyl-L-homocysteine
+
protein L-glutamate methyl ester

Synonyms

chemotaxis receptor methyltransferase, CheR, CheR1, CheR2, CheR3, EC 2.1.1.24, FrzF, glutamate-MTase, MCP methyltransferase I, MCP methyltransferase II, methyl-accepting chemotaxis protein methyltransferase II, methyl-accepting chemotaxis protein O-methyltransferase, methyltransferase CheR, methyltransferase, protein O-, O-MTase, OMTF3, PCMT, pentapeptide-independent chemotaxis receptor methyltransferase, protein carboxyl O-methyltransferase, protein carboxyl-methylase, protein carboxyl-O-methyltransferase, protein carboxylmethyltransferase II, protein carboxymethylase, protein carboxymethyltransferase, protein methylase II, protein methyltransferase, protein methyltransferase II, protein O-methyltransferase, protein(aspartate)methyltransferase, protein(carboxyl)methyltransferase, R0248, S-adenosylmethionine-glutamyl methyltransferase, S-adenosylmethionine:protein-carboxyl O-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.80 protein-glutamate O-methyltransferase

Purification

Purification on EC 2.1.1.80 - protein-glutamate O-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Phenyl-Sepharose, Bio-gel P-60, hydroxylapatite
-
ammonium sulfate, Sephadex G-75, SAH-Sepharose, Sephadex G-75
-
brain enzyme, pH 5.1 treatment, ammonium sulfate, SAH-Sepharose, Sephadex G-100
-
DEAE-Biogel, CM-Biogel, ammonium sulfate, S-adenosylhomocystein affinity column
-
HisTrap column chromatography
HisTrap column chromatography, and gel filtration
HisTrap HP nickel column chromatography
-
maltose binding protein-tagged enzyme is purified by Ni-NTA column chromatography
-
Ni-NTA column chromatography