2.1.1.82: 3-methylquercetin 7-O-methyltransferase
This is an abbreviated version!
For detailed information about 3-methylquercetin 7-O-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.82
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2.1.1.82
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benzylisoquinoline
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poppy
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opium
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norreticuline
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a-ring
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6-o-methyltransferase
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papaver
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papaverine
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somniferum
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isoflavonoids
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4\'omt
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flavanone
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daidzein
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7-o-methylation
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7-hydroxyl
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elicitor-induced
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polymethylated
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norcoclaurine
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coptis
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4\'-hydroxyl
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sakuranetin
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regio-specificity
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s-reticuline
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coclaurine
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aporphine
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antispasmodic
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americanum
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branch-point
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formononetin
- 2.1.1.82
-
benzylisoquinoline
- poppy
-
opium
- norreticuline
-
a-ring
-
6-o-methyltransferase
- papaver
- papaverine
- somniferum
-
isoflavonoids
-
4\'omt
- flavanone
- daidzein
-
7-o-methylation
-
7-hydroxyl
-
elicitor-induced
-
polymethylated
- norcoclaurine
-
coptis
-
4\'-hydroxyl
- sakuranetin
-
regio-specificity
-
s-reticuline
- coclaurine
-
aporphine
-
antispasmodic
- americanum
-
branch-point
- formononetin
Reaction
Synonyms
7-O-methyltransferase, 7-OMT, flavone 7-O-methyltransferase, flavonoid O-methyltransferase, flavonol 7-O-methyltransferase, methyltransferase, flavonol 7-, MT7, NOMT, OsOMT7
ECTree
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Engineering
Engineering on EC 2.1.1.82 - 3-methylquercetin 7-O-methyltransferase
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D257G
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the mutant methylates the 3-hydroxyl group of flavonols in addition to the 7-hydrdoxyl group
additional information
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generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene