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2.1.2.1: glycine hydroxymethyltransferase

This is an abbreviated version!
For detailed information about glycine hydroxymethyltransferase, go to the full flat file.

Word Map on EC 2.1.2.1

Reaction

5,10-methylenetetrahydrofolate
+
glycine
 +
H2O
=
tetrahydrofolate
 +
L-serine

Synonyms

AtSHMT3, bsSHMT, bstSHMT, EC 4.1.2.6, eSHMT, GlyA, GlyA protein, glycine hydroxymethyltransferrase, hSHMT, L-serine hydroxymethyltransferase, mitochondrial serine hydroxymethyltransferase, MJ1597, PvSHMT, Rhg4, serine hydroxymethyl transferase, serine hydroxymethylase hydroxymethyltransferase, serine, serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, serine hydroxymethyltransferase 2, serine hydroxymethyltransferase 2alpha, serine transhydroxymethylase, serine:H4F hydroxymethyltransferase, serine:tetrahydrofolate hydroxymethyltransferase, SHM1, SHM2, SHMT, SHMT-1, SHMT-L, SHMT-S, SHMT08, SHMT1, SHMT2, SHMT2alpha, SHMT3, zcSHMT, zebrafish cytosolic serine hydroxymethyltransferase, zebrafish mitochondiral serine hydroxymethyltransferase, zmSHMT

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.1 glycine hydroxymethyltransferase

pH Stability

pH Stability on EC 2.1.2.1 - glycine hydroxymethyltransferase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.5
resistant to alkaline denaturation up to, SHM1
659265
2 - 7
-
the enzyme retains more than 50% of its activity after 30 min at pH 2.0-7.0
755905
5.5 - 8.5
the enzyme retains over 80% of the maximum activity over a pH range from 7.0 to 8.5 for 24 h at 4°C, and more than 70% of the maximal activity at pH 6.5. However, the enzyme exhibits a rapid decrease in activity at pH 5.5
756288
5.5 - 9
purified recombinant SaSHMT shows significant stability under weakly acidic and alkaline environment at pH 6.5-9.0. Without any stabilizer at pH 6.5-9.0 for 96 h at 4°C over 80% of maximum activity is retained, at pH 5.5-6.0 less than 80% activity remains, the enzyme is unstable below pH 5.5
736753
5.8 - 8.5
-
stable, rabbit cytosol
441420
6 - 7.5
-
stable
441410
6 - 8
-
purified recombinant enzyme, over 60% maximal activity remains after 24 h at pH 6.0-7.5, 4°C, less than 20% of maximal activity at pH 8.0
736645
6 - 8.5
-
stable
441412
7.2 - 8.3
-
stable, at pH 8.55: loss of activity, tetrameric holoenzyme dissociates into the dimeric form, at pH 9.3: complete dissociation
441426
8
transition of SHM2 is between pH 6.5 and 11 and centered at about pH 8.0
659265
9
native SHM2 dimer dissociates into monomer
659265
9.25
transition of SHM1 is between pH 8 and 11 and centered at about pH 9.25
659265
additional information