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2.1.2.11: 3-methyl-2-oxobutanoate hydroxymethyltransferase

This is an abbreviated version!
For detailed information about 3-methyl-2-oxobutanoate hydroxymethyltransferase, go to the full flat file.

Word Map on EC 2.1.2.11

Reaction

5,10-methylenetetrahydrofolate
+
3-methyl-2-oxobutanoate
+
H2O
=
tetrahydrofolate
+
2-dehydropantoate

Synonyms

5,10-methylene tetrahydrofolate:alpha-ketoisovalerate hydroxymethyltransferase, alpha-ketoisovalerate hydroxymethyltransferase, dehydropantoate hydroxymethyltransferase, hydroxymethyltransferase, ketopantoate, ketopantoate hydroxymethyl transferase, ketopantoate hydroxymethyltransferase, KHMT, KPHMT, MOHMT, oxopantoate hydroxymethyltransferase, PanB

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.11 3-methyl-2-oxobutanoate hydroxymethyltransferase

Molecular Weight

Molecular Weight on EC 2.1.2.11 - 3-methyl-2-oxobutanoate hydroxymethyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
gel filtration
174000
-
recombinant enzyme, gel filtration
255000
-
sedimentation equilibrium
25700
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis
27000
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis
28179
-
6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE
285000
-
gel filtration
29000
29202
-
recombinant enzyme expressed in Escherichia coli BL21 (DE3): 4 * 29000, SDS-PAGE, 4 * 29202, electrospray ionization/mass spectrometry, 4 * 29366, calculated from the amino acid sequence
29337
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE
29366
-
recombinant enzyme expressed in Escherichia coli BL21 (DE3): 4 * 29000, SDS-PAGE, 4 * 29202, electrospray ionization/mass spectrometry, 4 * 29366, calculated from the amino acid sequence
340000
recombinant enzyme expressed in Escherichia coli, gel filtration
35000
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE
37700
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE
40000
8 * 37700, calculated from the amino acid sequence, 8 * 40000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE