Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.2.2: phosphoribosylglycinamide formyltransferase 1

This is an abbreviated version!
For detailed information about phosphoribosylglycinamide formyltransferase 1, go to the full flat file.

Word Map on EC 2.1.2.2

Reaction

10-formyltetrahydrofolate
+
N1-(5-phospho-D-ribosyl)glycinamide
=
tetrahydrofolate
+
N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Synonyms

2-amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase, 2-amino-N-ribosylacetamide 5'-phosphate transformylase, 5'-phosphoribosylglycinamide transformylase, 5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase, ADE8, GAR formyltransferase, GAR synthetase, GAR TFase, GAR transformylase, GARFT, GARFTase, GART, GART synthetase, GARTfase, glycinamide ribonucleotide formyl transferase, glycinamide ribonucleotide formyltransferase, glycinamide ribonucleotide transformylase, glycinamide ribotide formyltransferase, N10-formyltetrahydrolate:2-amino-N-ribosylacetamide-5'-phosphate transformylase, phosphoribosylglycinamide formyltransferase, PurN, purN transformylase, trifunctional purine biosynthetic protein adenosine-3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.2 phosphoribosylglycinamide formyltransferase 1

Cloned

Cloned on EC 2.1.2.2 - phosphoribosylglycinamide formyltransferase 1

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA, recombinant enzyme expressed in insect cells with a recombinant baculovirus
-
cloned and expressed in Escherichia coli BL21(DE3)pLysS
-
cloned and expressed in Escherichia coli HMS174(DE3)/pCC12
-
cloned by functional complementation in Escherichia coli
-
cloning of a 12-gene cluster encoding 9 enzymes for de novo purine nucleotide synthesis
-
cloning, chromosomal assignment, genomic organization and the complete sequence of the bovine GART gene
coding region of Escherichia coli GART amplified from chromosomal DNA with PCR method
-
enzyme expression analysis in hepatocellular carcinoma cell lines, overview
expressed in Escherichia coli Rosetta (DE3)pLysS cells
-
expression in Escherichia coli
gene purN, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
harboring expression vector pJS167, encoding the purN gene
-
hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202), GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202)
-
hybrid glycinamide ribonucleotide transformylase, ribonucleotide binding domain from purN, catalytic machinery and formyl tetrahydrofolate binding domain from purU encoded formyl tetrahydrofolate hydrolase
-
into the pET17b vector and into the pMC8HdeltaT vector for expression in Escherichia coli BL21Star(DE3)pRARE cells
-
pvdF gene characterization
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
second GAR transformylase encoded by the Escherichia coli purT gene
-