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2.1.2.2: phosphoribosylglycinamide formyltransferase 1

This is an abbreviated version!
For detailed information about phosphoribosylglycinamide formyltransferase 1, go to the full flat file.

Word Map on EC 2.1.2.2

Reaction

10-formyltetrahydrofolate
+
N1-(5-phospho-D-ribosyl)glycinamide
=
tetrahydrofolate
+
N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide

Synonyms

2-amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase, 2-amino-N-ribosylacetamide 5'-phosphate transformylase, 5'-phosphoribosylglycinamide transformylase, 5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase, ADE8, GAR formyltransferase, GAR synthetase, GAR TFase, GAR transformylase, GARFT, GARFTase, GART, GART synthetase, GARTfase, glycinamide ribonucleotide formyl transferase, glycinamide ribonucleotide formyltransferase, glycinamide ribonucleotide transformylase, glycinamide ribotide formyltransferase, N10-formyltetrahydrolate:2-amino-N-ribosylacetamide-5'-phosphate transformylase, phosphoribosylglycinamide formyltransferase, PurN, purN transformylase, trifunctional purine biosynthetic protein adenosine-3

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.2 phosphoribosylglycinamide formyltransferase 1

Molecular Weight

Molecular Weight on EC 2.1.2.2 - phosphoribosylglycinamide formyltransferase 1

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
102000
-
1 * 102000, SDS-PAGE
108000
-
human GART comprises the C-terminal domain of a 108 kDa, trifunctional enzyme
110000
110000 - 120000
-
gel filtration, sucrose density gradient ultracentrifugation, sedimentation velocity
11200
-
1 * 12100 + 1 * 11200, heterodimer, SDS-PAGE
112000
-
HeLa and L1210 cells
115000
-
-
117000
-
ultracentrifugation
12100
-
1 * 12100 + 1 * 11200, heterodimer, SDS-PAGE
125000
-
sucrose density gradient ultracentrifugation
140000
-
sucrose density ultracentrifugation
150000
160000
-
trifunctional protein, sucrose density gradient ultracentrifugation
190000
-
trifunctional protein, sucrose density gradient ultracentrifugation
201000
21060
-
calculated from peptide sequence deduced from the cDNA
21700
x * 21700, calculated, x * 26000, SDS-PAGE of recombinant protein including His-tag
21800
-
recombinant enzyme, predicted from cDNA
218000
21840
-
recombinant enzyme, MALDI-TOF mass spectral analysis
23000
23240
24000
-
equilibrium sedimentation ultracentrifugation
25700
-
SDS-PAGE
26000
x * 21700, calculated, x * 26000, SDS-PAGE of recombinant protein including His-tag
30985
-
x * 30985, calculated
31000
-
DNA sequencing
41900
-
product of the purT gene, SDS-PAGE
42000
-
product of the purT gene, deduced from predicted amino acid sequence
42400
-
product of the purT gene
55000
-
2 * 55000, SDS-PAGE, sucrose density ultracentrifugation, proteolytic cleavage
61000
63000
-
2 * 63000, transformylase, homodimer, SDS-PAGE
70000
-
gel filtration
83000
-
2 * 83000, trifunctional protein, SDS-PAGE
95000
-
2 * 95000, trifunctional protein, SDS-PAGE