2.1.2.8: deoxycytidylate 5-hydroxymethyltransferase

This is an abbreviated version!
For detailed information about deoxycytidylate 5-hydroxymethyltransferase, go to the full flat file.

Reaction

Synonyms

d-cytidine 5'-monophosphate hydroxymethylase, DCH, dCMP Hmase, dCMP hydroxymethylase, deoxyCMP hydroxymethylase, deoxycytidylate hydroxymethylase, deoxycytidylate hydroxymethyltransferase, deoxycytidylic hydroxymethylase, hydroxymethyltransferase, deoxycytidylate

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.8 deoxycytidylate 5-hydroxymethyltransferase

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Results	in table
1	Activating Compound
39	AA Sequence
1	CAS Registry Number
14	Cloned(Commentary)
33	Cofactor
2	Crystallization (Commentary)
13	Inhibitors
8	Ki Value [mM]
2	KM Value [mM]
1	Localization
17	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
9	PDB ID
1	pH Optimum
17	Protein Variants
11	Purification (Commentary)
7	Reaction
1	Reaction Type
18	Reference
7	Specific Activity [micromol/min/mg]
3	Storage Stability
37	Substrates and Products (Substrate)
8	Subunits
14	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
5	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.2.8 - deoxycytidylate 5-hydroxymethyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C148G

Tequatrovirus T4

-

mutant is unable to catalyze a detectable 18O exchange in either the absence or presence of the folate cofactor

485790

C148S/D179N

Tequatrovirus T4

P08773

active site mutant

757025

D145N

Tequatrovirus T4

P08773

the mutant shows no activity

757025

D179A

Tequatrovirus T4

-

mutant not inactivated by 5-fluoro-dUMP

485789

D179N

4 entries

D179S

Tequatrovirus T4

-

mutant not inactivated by 5-fluoro-dUMP

485789

E60D

Tequatrovirus T4

-

mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis

485791

E60Q

Tequatrovirus T4

-

mutant with decreased enzyme activity, impaired enzyme function in vitro and in vivo, uncoupling of early steps from later steps in catalysis

485791

S94A

Tequatrovirus T4

P08773

the mutant 25% residual activity compared to the wild type enzyme

757025

S94N

Tequatrovirus T4

P08773

the mutant shows no activity

757025

Y96F

Tequatrovirus T4

P08773

the mutant shows no activity

757025

additional information

2 entries

D179N

Tequatrovirus T4

-

mutant with altered substrate preference, favoring dUMP rather than dCMP

485789, 485790

D179N

Tequatrovirus T4

P08773

mutant with altered substrate preference, favoring dUMP rather than dCMP

485792

D179N

Tequatrovirus T4

-

mutation of active site, 10fold increased rate of inhibition by 5-fluoro-dUMP

485789

D179N

Tequatrovirus T4

P08773

the mutation significantly decreases the binding affinity to dCMP

757025

additional information

Tequatrovirus T4

-

transition mutations in gene 42

485783

additional information

Tequatrovirus T4

-

gene 42 amber mutants synthesize truncated forms of enzyme

485788