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2.1.2.9: methionyl-tRNA formyltransferase

This is an abbreviated version!
For detailed information about methionyl-tRNA formyltransferase, go to the full flat file.

Word Map on EC 2.1.2.9

Reaction

10-formyltetrahydrofolate
+
L-methionyl-tRNAfMet
=
tetrahydrofolate
 +
N-formylmethionyl-tRNAfMet

Synonyms

FMT, FMT1, Fmt1p, formyl-methionyl transferase, formylmethionyl-transfer ribonucleic synthetase, methionyl ribonucleic formyltransferase, methionyl-transfer ribonucleic transformylase, methionyl-transfer RNA transformylase, methionyl-tRNA formyltransferase, methionyl-tRNA Met formyltransferase, methionyl-tRNA transformylase, methionyl-tRNA-Formyltransferase, methyltransferase, methionyl-transfer ribonucleate, mitochondrial methionyl-tRNA formyltransferase, MTF, MTFMT, N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.9 methionyl-tRNA formyltransferase

Cloned

Cloned on EC 2.1.2.9 - methionyl-tRNA formyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate
-
cloning of the fmt gene and expression of wild-type and mutant genes in Escherichia coli BL21(DE3) as His6-tagged proteins
-
expression in Escherichia coli
-
expression of wild-type and of R42K, R42L and G41R/R42L mutant enzymes in Escherichia coli JM109 as His6-tagged proteins
-
fmt gene encoding enzyme is cloned
fmt gene encoding MTF is cloned and sequenced, expression in Escherichia coli JM109 as His6-tagged enzyme
-
gene encoding mitochondrial enzyme is cloned and expressed in Escherichia coli BL21(DE3) as His-tagged enzyme
-
gene encoding MTF is cloned and sequenced, expression of wild-type and mutant enzymes in Escherichia coli
possesses a single gene coding for each FMT and peptide deformylase, PDF, respectively, and both gene products are predicted to carry a bipartite targeting signal
-
wild-type and mutant fmt gene encoding MTF is cloned, overexpression of wild-type enzyme in Escherichia coli Novablue
-