2.1.2.9: methionyl-tRNA formyltransferase

This is an abbreviated version!
For detailed information about methionyl-tRNA formyltransferase, go to the full flat file.

Reaction

Synonyms

FMT, FMT1, Fmt1p, formyl-methionyl transferase, formylmethionyl-transfer ribonucleic synthetase, methionyl ribonucleic formyltransferase, methionyl-transfer ribonucleic transformylase, methionyl-transfer RNA transformylase, methionyl-tRNA formyltransferase, methionyl-tRNA Met formyltransferase, methionyl-tRNA transformylase, methionyl-tRNA-Formyltransferase, methyltransferase, methionyl-transfer ribonucleate, mitochondrial methionyl-tRNA formyltransferase, MTF, MTFMT, N10-formyltetrahydrofolic-methionyl-transfer ribonucleic transformylase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.2 Hydroxymethyl-, formyl- and related transferases

                2.1.2.9 methionyl-tRNA formyltransferase

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Results	in table
2	Activating Compound
46946	AA Sequence
1	CAS Registry Number
14	Cloned(Commentary)
29	Cofactor
7	Crystallization (Commentary)
77	Disease/ Diagnostics
18	General Information
3	General Stability
17	Inhibitors
5	Ki Value [mM]
57	KM Value [mM]
27	Localization
27	Metals/Ions
11	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
337	Organism
3	Pathway
18	PDB ID
5	pH Optimum
3	pH Range
71	Protein Variants
18	Purification (Commentary)
1	Reaction
1	Reaction Type
58	Reference
5	Source Tissue
12	Specific Activity [micromol/min/mg]
8	Storage Stability
239	Substrates and Products (Substrate)
5	Subunits
33	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
32	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.1.2.9 - methionyl-tRNA formyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

10-formyltetrahydrofolate + bovine mitochondrial L-methionyl-tRNA

tetrahydrofolate + bovine mitochondrial N-formylmethionyl-tRNA

Bos taurus

-

-

485807

-

-

r

10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet

tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet

2 entries

10-formyltetrahydrofolate + E. coli L-methionyl-tRNA1Val(CAU)

tetrahydrofolate + E. coli N-formylisoleucyl-tRNA1Val(CAU)

Bos taurus

-

-

485808

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet

3 entries

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

3 entries

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet

tetrahydrofolate + E. coli N-formylmethionyl-tRNAmMet

Bos taurus

-

-

485807, 485808

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAmMet

tetrahydrofolate + N-formylmethionyl-tRNAmMet

Bos taurus

-

-

485808

-

-

r

10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet

tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet

2 entries

10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet

tetrahydrofolate + N-formylvalyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + E.coli L-methionyl-tRNAfMet

tetrahydrofolate + E.coli N-formylmethionyl-tRNAfMet

Haemophilus influenzae

-

-

485814

-

-

r

10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

4 entries

10-formyltetrahydrofolate + L-isoleucyl-tRNAfMet

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

15 entries

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

114 entries

10-formyltetrahydrofolate + L-methionyl-tRNAMet

tetrahydrofolate + N-formylmethionyl-tRNAMet

2 entries

10-formyltetrahydrofolate + L-phenylalanyl-tRNAfMet + H2O

tetrahydrofolate + N-formylphenylalanyl-tRNAfMet

Escherichia coli

-

mutant tRNA G34A36 is a good substrate

657992

-

-

?

10-formyltetrahydrofolate + methionyl-tRNA1Val(CAU) + H2O

tetrahydrofolate + N-formylmethionyl-tRNA1Val(CAU)

Bos taurus

-

mischarged E. coli tRNA1Val(CAU), less efficient than with methionyl-tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + methionyl-tRNAmMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAmMet

Bos taurus

-

E. coli elongator Met-tRNAmMet, less efficient than with methionyl-tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O

tetrahydrofolate + N-formylphenylalanyl-tRNAfMet

3 entries

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

9 entries

10-formyltetrahydropteroyltriglutamate + L-methionyl-tRNAfMet + H2O

tetrahydropteroyltriglutamate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis

-

10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can act as donor

485802

-

485802

?

additional information

?

-

61 entries

10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet

tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet

Bos taurus

-

-

485808

-

-

r

10-formyltetrahydrofolate + E. coli L-isoleucyl-tRNAfMet

tetrahydrofolate + E. coli N-formylisoleucyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet

Bos taurus

-

-

485807, 485808

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

-

485812

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

-

485808

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Haemophilus influenzae

-

-

485814

-

-

r

10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet

tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet

Bos taurus

-

-

485808

-

-

r

10-formyltetrahydrofolate + E. coli L-valyl-tRNAfMet

tetrahydrofolate + E. coli N-formylvalyl-tRNAfMet

Saccharomyces cerevisiae

-

-

485813

-

-

r

10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

Bos taurus

-

mischarged E. coli tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

Bos taurus

-

less efficient than with methionyl-tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

Saccharomyces cerevisiae

-

mischarged E. coli tRNAfMet

485813

-

-

?

10-formyltetrahydrofolate + isoleucyl-tRNAfMet + H2O

tetrahydrofolate + N-formylisoleucyl-tRNAfMet

Saccharomyces cerevisiae

-

250fold reduced catalytic efficiency compared with methionyl-tRNAfMet

485813

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis

-

-

485802

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

-

485795, 485796, 485797, 485799, 485809, 485810, 485811

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

-

736461

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

-

485812

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

Escherichia coli tRNA2fMet

736461

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

-

485810

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

-

485810

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Homo sapiens

-

-

719239, 757809

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Homo sapiens

Q96DP5

-

736461

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Homo sapiens

Q96DP5

human mt-tRNAMet

736461

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

-

756708

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Staphylococcus aureus

-

-

735441

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Staphylococcus aureus WCUH29

-

-

735441

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Toxoplasma gondii

-

-

720491

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Toxoplasma gondii RH

-

-

720491

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate

485807

-

485807

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

formylates bovine mitochondrial and E. coli initiator Met-tRNA with equal efficiency, formylates E. coli elongator Met-tRNA at a lower rate

485808

-

485808

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

formylates methionyl-tRNAfMet from E. coli

485807

-

485807

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

enzyme preferentially recognizes the methionyl moiety of its tRNA substrate, small importance of the tRNA acceptor stem for recognition, E. coli tRNA mutants as substrates

485808

-

485808

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

broader substrate specificity than that of E. coli enzyme

485807

-

485807

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

broader substrate specificity than that of E. coli enzyme

485808

-

485808

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Bos taurus

-

recognition mechanism of methionyl-tRNAfMet

485808

-

485808

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis

-

10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis

-

specificity is restricted to methionyl-tRNAfMet

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis

-

involved in initiation of protein biosynthesis

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis R / ATCC 8043

-

10-formyltetrahydropteroyltriglutamate or 10-formyltetrahydrofolate can also act as donor

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis R / ATCC 8043

-

specificity is restricted to methionyl-tRNAfMet

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Enterococcus faecalis R / ATCC 8043

-

involved in initiation of protein biosynthesis

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

-

661778

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

determinants for formylation are located in the acceptor stem and D-stem of the initiator tRNA, enzyme binds to the tRNA substrate in an induced fit mechanism, tRNA also undergoes induced structural changes during its binding to enzyme

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

role of the linker region between N- and C-terminal domain

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity determinants at the end of the acceptor stem of tRNAfMet

485798, 485799

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity determinants at the end of the acceptor stem of tRNAfMet

485811

-

485811

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

specificity determinants at the end of the acceptor stem of tRNAfMet

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

affinity of tRNAfMet for enzyme is improved by aminoacylation

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

affinity of tRNAfMet for enzyme is improved by aminoacylation

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

formylates methionyl-tRNAfMet from E. coli

485794

-

485794

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

formylates methionyl-tRNAfMet from E. coli

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

formylates methionyl-tRNAfMet from E. coli

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

formylates methionyl-tRNAfMet from E. coli

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

formylates methionyl-tRNAfMet from E. coli

485798, 485799

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

invariant Arg-42 within the 16-amino acid insertion loop, residues 34-49, plays a very important role both in steps of substrate binding and in catalysis, Arg-42 makes functional contacts with the determinants in the acceptor stem of the initiator tRNA

485811

-

485811

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity is restricted to methionyl-tRNAfMet

485798, 485799

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity is restricted to methionyl-tRNAfMet

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity is restricted to methionyl-tRNAfMet

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specificity is restricted to methionyl-tRNAfMet

485811

-

485811

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

specificity is restricted to methionyl-tRNAfMet

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

specificity is restricted to methionyl-tRNAfMet

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

nucleotide-mutants of tRNAfMet as substrates

485798, 485799

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

tRNA substrate binding

485806

-

485806

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

tRNA substrate binding

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

recognition mechanism of methionyl-tRNAfMet

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

recognition mechanism of methionyl-tRNAfMet

485805

-

485805

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

recognition mechanism of methionyl-tRNAfMet

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485811

-

485811

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

keeps in store initiator tRNAfMet for initiation of protein synthesis

485798

-

485798

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485798

-

485798

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

involved in initiation of protein biosynthesis

485811

-

485811

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

involved in initiation of protein biosynthesis

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

involved in initiation of protein biosynthesis

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

formyl group provides a positive determinant for IF2, which can select the initiator fMet-tRNA from other tRNAs and a negative determinant, which blocks the binding of EF-Tu to the initiator tRNA

485815

-

485815

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

P23882

enzyme reaction irreversibly commits methionyl-tRNAfMet to initiation of translation in eubacteria

485805

-

485805

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

key step in the targeting of initiator tRNA towards the translation start machinery in prokaryotes

485806

-

485806

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli

-

small effect of overproduction of MRF on activity of chloramphenicol acetyltransferase

657992

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli A19

-

specificity is restricted to methionyl-tRNAfMet

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli A19

-

involved in initiation of protein biosynthesis

485802

-

485802

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli B / ATCC 11303

-

formylates methionyl-tRNAfMet from E. coli

485794

-

485794

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

recognition mechanism of methionyl-tRNAfMet

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

involved in initiation of protein biosynthesis

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

specificity is restricted to methionyl-tRNAfMet

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli CA274

-

involved in initiation of protein biosynthesis

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli DH5-alphaF

-

recognition mechanism of methionyl-tRNAfMet

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli DH5-alphaF

-

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli DH5-alphaF

-

involved in initiation of protein biosynthesis

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

recognition mechanism of methionyl-tRNAfMet

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

important role of a 16-amino acid insertion loop in enzyme for tRNA recognition

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

involved in initiation of protein biosynthesis

485809

-

485809

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

specificity is restricted to methionyl-tRNAfMet

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

-

involved in initiation of protein biosynthesis

485810

-

485810

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

P23882

role of the linker region between N- and C-terminal domain

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

P23882

specificity determinants at the end of the acceptor stem of tRNAfMet

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli JM109

P23882

C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 EM20031(F32)

-

affinity of tRNAfMet for enzyme is improved by aminoacylation

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 EM20031(F32)

-

1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 EM20031(F32)

-

formylates methionyl-tRNAfMet from E. coli

485795

-

485795

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 EM20031(F32)

-

affinity of tRNAfMet for enzyme is improved by aminoacylation

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 EM20031(F32)

-

1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme

485797

-

485797

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 MO

-

formylates methionyl-tRNAfMet from E. coli

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 MO

-

specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli K12 MO

-

involved in initiation of protein biosynthesis

485796

-

485796

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli XL1-Blue

P23882

role of the linker region between N- and C-terminal domain

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli XL1-Blue

P23882

specificity determinants at the end of the acceptor stem of tRNAfMet

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Escherichia coli XL1-Blue

P23882

C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site

485812

-

485812

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis

-

formylates methionyl-tRNAfMet from Euglena gracilis or yeast

485800

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis

-

formylates methionyl-tRNAfMet from E. coli

485800

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis

-

high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate

485800, 485801

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis

-

involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes

485800

-

485800

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis Z

-

formylates methionyl-tRNAfMet from Euglena gracilis or yeast

485800

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis Z

-

formylates methionyl-tRNAfMet from E. coli

485800

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis Z

-

high specificity for initiator tRNAMet, strong positive cooperativity for Met-tRNAfMet and N10-formyltetrahydrofolate

485800

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Euglena gracilis Z

-

involved in initiation of protein synthesis in chloroplasts, other mechanism than in cytoplasm of eukaryotes

485800

-

485800

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Haemophilus influenzae

-

formylates methionyl-tRNAfMet from E. coli

485814

-

485814

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Haemophilus influenzae

-

Asn-107, His-109 and Asp-145 are catalytic residues, Arg-41 is involved in tRNA recognition

485814

-

485814

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Pseudomonas aeruginosa

-

E. coli tRNAFMet

661778

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

P32785

-

673867

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

formylates yeast mitochondrial methionyl-tRNAfMet, specific for initiator tRNAs

485813

-

485813

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

formylates methionyl-tRNAfMet from E. coli

485813

-

485813

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

enzyme recognizes nucleotidic features in the acceptor stem of mitochondrial initiator tRNA, recognition mechanism

485813

-

485813

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

-

initiation of protein synthesis in mitochondria

485813

-

485813

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Saccharomyces cerevisiae

P32785

the dispensable mitochondrial methionyl-tRNAformyltransferase, Fmt1p, is essential for respiratory growth in the absence of Rsm28p

673867

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Triticum aestivum

-

formylates methionyl-tRNAfMet from wheat germ and leaf

485804

-

485804

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Triticum aestivum

-

formylates methionyl-tRNAfMet from E. coli

485804

-

485804

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Triticum aestivum

-

component of a protein chain initiating system in chloroplasts

485804

-

485804

?

10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O

tetrahydrofolate + N-formylmethionyl-tRNAfMet

Trypanosoma brucei

-

formylation of the methionyl-tRNA-fMet is essential for in vitro binding of imported formylated elongator-type tRNA(Met)

659462

-

-

?

10-formyltetrahydrofolate + L-methionyl-tRNAMet

tetrahydrofolate + N-formylmethionyl-tRNAMet

Escherichia coli

-

-

485794

-

-

r

10-formyltetrahydrofolate + L-methionyl-tRNAMet

tetrahydrofolate + N-formylmethionyl-tRNAMet

Escherichia coli B / ATCC 11303

-

-

485794

-

-

r

10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O

tetrahydrofolate + N-formylphenylalanyl-tRNAfMet

Escherichia coli

-

mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O

tetrahydrofolate + N-formylphenylalanyl-tRNAfMet

Escherichia coli

-

7fold less efficient formylation than of methionyl-tRNAfMet

485798

-

-

?

10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O

tetrahydrofolate + N-formylphenylalanyl-tRNAfMet

Escherichia coli K12 MO

-

mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Bos taurus

-

mischarged E. coli tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Bos taurus

-

less efficiency than with methionyl-tRNAfMet

485808

-

-

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Escherichia coli

-

mischarged E. coli tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Escherichia coli

-

mischarged E. coli tRNAfMet

485798

-

-

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Escherichia coli

-

130fold less efficient formylation than of methionyl-tRNAfMet

485798

-

-

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Escherichia coli

-

reduced velocity compared to the formylation of methionyl-tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Escherichia coli K12 MO

-

mischarged E. coli tRNAfMet

485796

-

485796

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Saccharomyces cerevisiae

-

mischarged E. coli tRNAfMet

485813

-

-

?

10-formyltetrahydrofolate + valyl-tRNAfMet + H2O

tetrahydrofolate + N-formylvalyl-tRNAfMet

Saccharomyces cerevisiae

-

25fold reduced catalytic efficiency compared with methionyl-tRNAfMet

485813

-

-

?

additional information

?

-

Bos taurus

-

no formylation of Val-tRNA1Met

485808

-

-

?

additional information

?

-

Bos taurus

-

translational initiation in mitochondria

485807

-

-

?

additional information

?

-

Bos taurus

-

translational initiation in mitochondria

485808

-

-

?

additional information

?

-

Enterococcus faecalis

-

no formylation of methionyl-tRNAmMet

485802

-

-

?

additional information

?

-

Enterococcus faecalis

-

ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet

485802

-

-

?

additional information

?

-

Enterococcus faecalis R / ATCC 8043

-

no formylation of methionyl-tRNAmMet

485802

-

-

?

additional information

?

-

Enterococcus faecalis R / ATCC 8043

-

ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet

485802

-

-

?

additional information

?

-

Escherichia coli

-

no formyl group donor: 5-formyltetrahydrofolate

485797

-

-

?

additional information

?

-

Escherichia coli

-

mutant tRNAs as poor substrates

485809

-

-

?

additional information

?

-

Escherichia coli

-

mutant tRNAs as poor substrates

485810

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485795

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485797

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485798

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485802

-

-

?

additional information

?

-

Escherichia coli

P23882

no formylation of methionyl-tRNAmMet

485812

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485812

-

-

?

additional information

?

-

Escherichia coli

P23882

no formylation of methionyl-tRNAmMet

485815

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of methionyl-tRNAmMet

485815

-

-

?

additional information

?

-

Escherichia coli

-

broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet

485795

-

-

?

additional information

?

-

Escherichia coli

-

no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA

485794

-

-

?

additional information

?

-

Escherichia coli

-

ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet

485802

-

-

?

additional information

?

-

Escherichia coli

-

enzyme structure

485809

-

-

?

additional information

?

-

Escherichia coli

-

enzyme structure

485810

-

-

?

additional information

?

-

Escherichia coli

-

enzyme structure

485811

-

-

?

additional information

?

-

Escherichia coli

P23882

enzyme structure

485812

-

-

?

additional information

?

-

Escherichia coli

-

enzyme structure

485812

-

-

?

additional information

?

-

Escherichia coli

P23882

enzyme structure

485815

-

-

?

additional information

?

-

Escherichia coli

-

enzyme structure

485815

-

-

?

additional information

?

-

Escherichia coli

-

no formyl group donor: 5,10-methenyltetrahydrofolate

485794

-

-

?

additional information

?

-

Escherichia coli

-

no formyl group donor: 5,10-methenyltetrahydrofolate

485797

-

-

?

additional information

?

-

Escherichia coli A19

-

no formylation of methionyl-tRNAmMet

485802

-

-

?

additional information

?

-

Escherichia coli A19

-

ATP plus L-methionine and either tRNAmMet or tRNAfMet cannot replace methionyl-tRNAfMet

485802

-

-

?

additional information

?

-

Escherichia coli B / ATCC 11303

-

no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA

485794

-

-

?

additional information

?

-

Escherichia coli B / ATCC 11303

-

no formyl group donor: 5,10-methenyltetrahydrofolate

485794

-

-

?

additional information

?

-

Escherichia coli CA274

-

mutant tRNAs as poor substrates

485809

-

-

?

additional information

?

-

Escherichia coli CA274

-

enzyme structure

485809

-

-

?

additional information

?

-

Escherichia coli CA274

-

mutant tRNAs as poor substrates

485810

-

-

?

additional information

?

-

Escherichia coli CA274

-

enzyme structure

485810

-

-

?

additional information

?

-

Escherichia coli DH5-alphaF

-

mutant tRNAs as poor substrates

485809

-

-

?

additional information

?

-

Escherichia coli DH5-alphaF

-

enzyme structure

485809

-

-

?

additional information

?

-

Escherichia coli JM109

-

mutant tRNAs as poor substrates

485809

-

-

?

additional information

?

-

Escherichia coli JM109

-

enzyme structure

485809

-

-

?

additional information

?

-

Escherichia coli JM109

-

mutant tRNAs as poor substrates

485810

-

-

?

additional information

?

-

Escherichia coli JM109

-

enzyme structure

485810

-

-

?

additional information

?

-

Escherichia coli JM109

P23882

no formylation of methionyl-tRNAmMet

485812

-

-

?

additional information

?

-

Escherichia coli JM109

P23882

enzyme structure

485812

-

-

?

additional information

?

-

Escherichia coli K12 EM20031(F32)

-

no formylation of methionyl-tRNAmMet

485795

-

-

?

additional information

?

-

Escherichia coli K12 EM20031(F32)

-

broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet

485795

-

-

?

additional information

?

-

Escherichia coli K12 EM20031(F32)

-

no formyl group donor: 5-formyltetrahydrofolate

485797

-

-

?

additional information

?

-

Escherichia coli K12 EM20031(F32)

-

no formylation of methionyl-tRNAmMet

485797

-

-

?

additional information

?

-

Escherichia coli K12 EM20031(F32)

-

no formyl group donor: 5,10-methenyltetrahydrofolate

485797

-

-

?

additional information

?

-

Escherichia coli XL1-Blue

P23882

no formylation of methionyl-tRNAmMet

485812

-

-

?

additional information

?

-

Escherichia coli XL1-Blue

P23882

enzyme structure

485812

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

mutant tRNAs as poor substrates

485813

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

formylates mitochondrial methionyl-tRNAfMet from yeast, low-level activity with yeast cytoplasmic and E. coli tRNA

485803

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

mitochondrial protein synthesis

485803

-

-

?

additional information

?

-

Saccharomyces cerevisiae D243-2B-R1

-

formylates mitochondrial methionyl-tRNAfMet from yeast, low-level activity with yeast cytoplasmic and E. coli tRNA

485803

-

-

?

additional information

?

-

Saccharomyces cerevisiae D243-2B-R1

-

mitochondrial protein synthesis

485803

-

-

?

additional information

?

-

Salmonella enterica

-

resistance mutations are identified in the fmt gene encoding the enzyme methionyl-tRNA formyltransferase. In the absence of antibiotic, these resistance mutations confer a fitness cost that is manifested as a reduced growth rate in laboratory medium and in mice

676864

-

-

?

additional information

?

-

Triticum aestivum

-

no formylation of cytoplasmic initiating Met-tRNAi

485804

-

-

?

additional information

?

-

Triticum aestivum

-

no formylation of methionyl-tRNAmMet

485804

-

-

?