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2.1.3.1: methylmalonyl-CoA carboxytransferase

This is an abbreviated version!
For detailed information about methylmalonyl-CoA carboxytransferase, go to the full flat file.

Word Map on EC 2.1.3.1

Reaction

(S)-methylmalonyl-CoA
+
pyruvate
=
propanoyl-CoA
 +
oxaloacetate

Synonyms

5S subunit of transcarboxylase, carboxyltransferase subunit of acetyl-CoA carboxylase, carboxyltransferase, methylmalonyl coenzyme A, DtsR1, MCT, methyl malonyl CoA carboxyl transferase, methylmalonyl CoA carboxyltransferase, methylmalonyl CoA-oxalacetate transcarboxylase, methylmalonyl coenzyme A carboxyltransferase, methylmalonyl-CoA carboxyltransferase, methylmalonyl-CoA transcarboxylase, MMC, oxalacetic transcarboxylase, transcarboxylase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.1 methylmalonyl-CoA carboxytransferase

Crystallization

Crystallization on EC 2.1.3.1 - methylmalonyl-CoA carboxytransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DtsR1 is crystallized by the sitting-drop vapour-diffusion method using polyethylene glycol 6000 as a precipitant
-
5S-subunit, native, with His-tag and selenomethionine-protein with His-tag. Crystallization of native 5S subunit requires addition of lithium sulfate, with in turn prevents crystallization of His-tagged subunit
-
central 12S hexameric core, in complex with substrate methylmalonyl-CoA. Structure shows two stacked trimers, and a domain duplication in the monomer
of 5S metalloenzyme subunit, free and in complex with substrate pyruvate, product oxaloacetate, or inhibitor 2-ketobutyrate. Dimer of beta8alpha8 barrels with active site cobalt ion