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2.1.3.2: aspartate carbamoyltransferase

This is an abbreviated version!
For detailed information about aspartate carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.2

Reaction

Carbamoyl phosphate
+
L-aspartate
=
phosphate
 +
N-carbamoyl-L-aspartate

Synonyms

(S)-2-methyl-3-oxopropanoyl-CoA:pyruvate carboxyltransferase, ACT, aspartate carbamoyltransferase, aspartate carbamyltransferase, aspartate trans carbamoylase, aspartate transcarbamoylase, aspartate transcarbamylase, aspartic acid transcarbamoylase, aspartic carbamyltransferase, aspartic transcarbamylase, ATC, ATC domain of CAD, ATCase, CAD, carbamoylaspartotranskinase, carbamoyltransferase, aspartate, carbamylaspartotranskinase, L-aspartate transcarbamoylase, L-aspartate transcarbamylase, MJ1581, PYRB

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.2 aspartate carbamoyltransferase

Purification

Purification on EC 2.1.3.2 - aspartate carbamoyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, aspartate-Sepharose
-
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex, Sephadex G-200, partially purified
-
aspartate transcarbamoylase activity domain of the multienzyme complex
-
CaCl2, heat, ethanol, 10-carboxydecylamino-Sepharose, DEAE-cellulose
-
ethanol, 10-carboxydecyl-Sepharose, DEAE-cellulose
-
heat, ammonium sulfate, DEAE-cellulose
-
heat, Q-Sepharose, Phenyl-Sepharose
HiTrap heparin column chromatography
ion-exchange, gel filtration
-
MnSO4, ammonium sulfate, DEAE-cellulose, Sephadex G-100
-
multienzyme complex
native and recombinant enzyme
-
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
pH 4.8, hydroxylapatite, ammonium sulfate, DEAE-Sephadex
-
protamine sulfate, heat treatment, ammonium sulfate, Sephadex G-150, DEAE-Sephadex, DEAE-cellulose
-
purification steps at room temperatur
-
Q-Sepharose, Matrex gel Red A, Matrex Phenyl Cellufine
-
rapid procedure
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain EK1104 by ion exchange and hydrophobic interaction chromatography
recombinant enzyme from Escherichia coli strain EK114 by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography
recombinant enzyme from the N-terminal His6-tagged maltose-binding fusion protein CAD complex from Escherichia coli strain BL21(DE3) pLysS cells by ncikel affinity chromatography, ion-exchange chromatography, tag cleavage, and gel filtration. Three extra residues are left at the N-terminus of huATCase (GPM1915SP) after removal of the tag
recombinant enzyme, Ni2+-affinity column
-
recombinant His6-tagged enzyme
recombinant wild-type and mutant enzymes from Escherichia coli strain EK1104 by isoelectric precipitation, anion exchange, and hydrophobic interaction chromatography, and gel filtration
Strep-tag affinity resin column chromatography and Superdex 75 gel filtration
streptomycin sulfate, ammonium sulfate, DE-52 column, Sephadex G-200, aspartate linked CNBr
-
Streptomycin sulfate, ammonium sulfate, Sephadex G-200, hydroxylapatite, preparative gel electrophoresis
-