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2.1.3.3: ornithine carbamoyltransferase

This is an abbreviated version!
For detailed information about ornithine carbamoyltransferase, go to the full flat file.

Word Map on EC 2.1.3.3

Reaction

Carbamoyl phosphate
+
L-ornithine
=
phosphate
 +
L-citrulline

Synonyms

anabolic ornithine carbamoyltransferase, anabolic ornithine transcarbamoylase, AOTC, arcB gene product, ArgF, ArgK, aTtOTCase, carbamoyltransferase, carbamoyltransferase, ornithine, carbamylphosphate-ornithine transcarbamylase, catabolic ornithine carbamoyltransferase, catabolic ornithine transcarbamylase, citrulline phosphorylase, cOTC, L-ornithine carbamoyltransferase, L-ornithine carbamyltransferase, L-ornithine transcarbamylase, Lmo0036, MB1684, Mtb OTC, OCT, ornithine carbamoyltransferase, ornithine carbamoytransferase, ornithine carbamyl transferase, ornithine carbamyltransferase, ornithine transcarbamoylase, ornithine transcarbamylase, ornithine transcarbamylase 3, OTC, otcase, ROTCase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.3 Carboxy- and carbamoyltransferases
                2.1.3.3 ornithine carbamoyltransferase

Cloned

Cloned on EC 2.1.3.3 - ornithine carbamoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression with GroES and GroEL in Escherichia coli
-
enzyme protomer structure, overview
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) Tuner cells
expression in Escherichia coli
expression in Escherichia coli and in Saccharomyces cerevisiae
expression in Pseudomonas aeruginosa
-
expression in Saccharomyces cerevisiae
-
expression of isoform 1 and 2 in Escherichia coli
-
expression of wild-type, Y227C, A240D, E277G, Y227C/E277G and A240D/E277G mutant enzyme in Saccharomyces cerevisiae
-
gene argF, expression of His-tagged aOTC in Escherichia coli strain BL21-CodonPlus(DE3)-RIL
gene argK, overexpression of His-tagged enzyme
gene lmo0036, phylogenetic tree, quantitative real-time PCR expresion analysis at different acidic conditions, overview
-
gene MB1684, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
His-tag fusion protein expressed in Escherichia coli JM109(DE3) (pLys), recombinant protein is fully active
-
stable overexpression of arginase I and ornithine transcarbamylase in HepG2 cells using a eukaryotic dual gene expression vector pBudCE4.1, the co-expression improves ammonia detoxification of the cells, improvement of HepG2 cell function, overview
-
three different splicing isoforms, OTC-t1, OTC-t2, and OTC-t3, all three isoforms use a canonical AAUAAA hexamer that is predicted to fold into a hairpin secondary structure which might be exposed to the cleavage and polyadenylation specificity factor. In addition, the OTC-t1 and OTC-t2 transcripts display heterogeneity at the cleavage sites in a tissue-dependent manner. Secondary structure of the OTC mRNA polyA signals, overview