2.1.3.6: putrescine carbamoyltransferase
This is an abbreviated version!
For detailed information about putrescine carbamoyltransferase, go to the full flat file.
Reaction
Synonyms
anabolic putrescine carbamoyltransferase, aPTC, catabolic putrescine carbamoyltransferase, cPTC, Lmo0036, PTC, PTCase, putrescine carbamoyltransferase, putrescine synthase, putrescine transcarbamylase
ECTree
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Engineering
Engineering on EC 2.1.3.6 - putrescine carbamoyltransferase
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R54G
site-directed mutagenesis, inactive mutant, not exhibiting any PTC or OTC activity
Y230V/G231S/L232M/Y233G
engineering of the 230-loop of PTC, by replacing the sequence 230YGLY233 of the putrescine signature by its OTC counterpart VSMG, favors the use of ornithine and impairs that of putrescine
additional information
confirmation of decreased stability of the trimer of PTC lacking the C-terminal helix by deleting this helix
additional information
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construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036
additional information
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construction of a lmo0036 knockout mutant by homologous recombination using for in-frame deletion of the whole length of lmo0036
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