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2.1.4.1: glycine amidinotransferase

This is an abbreviated version!
For detailed information about glycine amidinotransferase, go to the full flat file.

Word Map on EC 2.1.4.1

Reaction

L-arginine
+
glycine
=
L-ornithine
 +
Guanidinoacetate

Synonyms

AGAT, arginine-glycine amidinotransferase, arginine-glycine transamidinase, arginine:glycine amidinotransferase, CyrA, EC 2.6.2.1, GAT, GATM, glycine amidinotransferase, glycine aminotransferase, glycine transamidinase, L-arginine:glycine amidinotransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.4 Amidinotransferases
                2.1.4.1 glycine amidinotransferase

Cloned

Cloned on EC 2.1.4.1 - glycine amidinotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA clone isolated
cDNA, nucleotide sequence determined
cDNA, nucleotide sequence determined, cloned and expressed in Escherichia coli BL21(DE3)-pLysS
for sequencing and synthesizing of RNA probes for in situ hybridizations
gene AGAT, recombinant expression in HEK-293 cells
gene cyrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of N-terminal His6-tagged CyrA in Escherichia coli strain BL21(DE3)
gene cyrA, expression of N-terminal His6-tagged CyrA in Escherichia coli strain BL21(DE3)
gene GATM, a differentially methylated region is detected in the eighth intron of the L-arginine:glycine amidinotransferase gene. The methylation in male primordial germ cells of 6-8-days-old embryos is higher than that in female primordial germ cells, genotyping, the GATM gene displays biallelic expression in primordial germ cells as well as somatic embryonic, extraembryonic, and adult chicken tissues, reverse-transcription PCR and quantitative real-time PCR expression analyses, sequence comparisons