2.1.4.1: glycine amidinotransferase

This is an abbreviated version!
For detailed information about glycine amidinotransferase, go to the full flat file.

Reaction

Synonyms

AGAT, arginine-glycine amidinotransferase, arginine-glycine transamidinase, arginine:glycine amidinotransferase, CyrA, EC 2.6.2.1, GAT, GATM, glycine amidinotransferase, glycine aminotransferase, glycine transamidinase, L-arginine:glycine amidinotransferase

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.4 Amidinotransferases

                2.1.4.1 glycine amidinotransferase

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Results	in table
7	Activating Compound
1310	AA Sequence
9	Application
1	CAS Registry Number
14	Cloned(Commentary)
1	Crystallization (Commentary)
165	Disease/ Diagnostics
5	Expression
16	General Information
50	Inhibitors
3	Ki Value [mM]
39	KM Value [mM]
25	Localization
34	Molecular Weight [Da]
96	Natural Substrates/ Products (Substrates)
583	Organism
8	Pathway
11	PDB ID
16	pH Optimum
4	pH Range
1	pH Stability
2	pI Value
9	Protein Variants
12	Purification (Commentary)
3	Reaction
6	Reaction Type
81	Reference
187	Source Tissue
13	Specific Activity [micromol/min/mg]
2	Storage Stability
246	Substrates and Products (Substrate)
21	Subunits
41	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Temperature Range [°C]
4	Temperature Stability [°C]
2	Turnover Number [1/s]

Engineering

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Engineering on EC 2.1.4.1 - glycine amidinotransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

F245N

Cylindrospermopsis raciborskii

B0LI36

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT. F245N variant seems to lack the potential ligand-induced conformational changes. Higher activity of the F245N variant is enthalpy-driven

719473

F245N/S247M

Cylindrospermopsis raciborskii

B0LI36

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT

719473

S247M

Cylindrospermopsis raciborskii

B0LI36

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT

719473

F245N

Cylindrospermopsis raciborskii AWT205

-

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT. F245N variant seems to lack the potential ligand-induced conformational changes. Higher activity of the F245N variant is enthalpy-driven

-

F245N/S247M

Cylindrospermopsis raciborskii AWT205

-

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT

-

S247M

Cylindrospermopsis raciborskii AWT205

-

site-directed mutagenesis, exchange analogously to the human L-arginine:glycine amidinotransferase, h-AGAT

-

medicine

Homo sapiens

-

in vitro assay for enzyme activity in lymphocytes based on stable-isotope-labeled substrates L-(guanidino-15N2)arginine and U-(13C,15N)glycine

661505

additional information

2 entries

additional information

Cylindrospermopsis raciborskii

B0LI36

the residue replacements do not change the kinetic mechanism of the three variant enzymes. The mutant protein variants have broad substrate specificity, e.g. the F245N variant additionally accepts creatine, with 7% relative activity

719473

additional information

Cylindrospermopsis raciborskii AWT205

-

the residue replacements do not change the kinetic mechanism of the three variant enzymes. The mutant protein variants have broad substrate specificity, e.g. the F245N variant additionally accepts creatine, with 7% relative activity

-