2.2.1.10: 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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For detailed information about 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase, go to the full flat file.

Reaction

Synonyms

ADHS, 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, ADH synthase, ADHS, MJ0400, More

ECTree

     2 Transferases

         2.2 Transferring aldehyde or ketonic groups

             2.2.1 Transketolases and transaldolases

                2.2.1.10 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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Results	in table
1942	AA Sequence
3	Cloned(Commentary)
1	Crystallization (Commentary)
4	General Information
2	Inhibitors
1	Ki Value [mM]
1	KM Value [mM]
3	Natural Substrates/ Products (Substrates)
3	Organism
6	Pathway
2	pH Optimum
3	Purification (Commentary)
2	Reaction
3	Reference
1	Source Tissue
1	Specific Activity [micromol/min/mg]
7	Substrates and Products (Substrate)
2	Subunits
9	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]

Crystallization

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Crystallization on EC 2.2.1.10 - 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant detagged ADH synthase in complex with substrate analogue fructose 1,6-bisphosphate, with dihydroxyacetone phosphate, and with native structure-containing copurified ligands, modeled as dihydroxyacetone phosphate and glycerol, vapor diffusion hanging drop method, mixing of 0.002 ml of protein solution containing 20 mg/ml protein in 10 mM Tris, pH 7.5, with 0.002 ml of reservoir solution containing 4-8% 1,4-butanediol and 0.1 M acetate, pH 4.2-4.3, 1-2-days, soaking of crystals in motherliquor with 10 mM ligands, for 1 h, X-ray diffraction structure determination and analysis at 2.6-2.8 A resolution

Methanocaldococcus jannaschii

Q57843

718845