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2.2.1.2: transaldolase

This is an abbreviated version!
For detailed information about transaldolase, go to the full flat file.

Word Map on EC 2.2.1.2

Reaction

sedoheptulose 7-phosphate
+
D-glyceraldehyde 3-phosphate
=
D-erythrose 4-phosphate
 +
D-fructose 6-phosphate

Synonyms

824-TAL, 824-WT, ATAL, Atalp, dihydroxyacetone synthase, dihydroxyacetonetransferase, formaldehyde transketolase, FoTal, hTAL, NQM1/YGR043C, peroxisomal transaldolase, TAL, Tal1, TAL2, TALase, TALB, TALDO, TALDO1, transaldolase, transaldolase 1, transaldolase A, transaldolase B, transaldolase STY3758, transaldolase1, transladolase B, XIF1

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.2 transaldolase

Crystallization

Crystallization on EC 2.2.1.2 - transaldolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallization with a combination of micro- and macro-seeding techniques, space group: P212121
-
mutant enzyme F178Y with bound D-tagatose 6-phosphate , hanging drop vapor diffusion method, using 0.15 M Li2SO4, 18% (w/v) PEG 3350. Mutant enzyme E96Q with bound D-fructose 6-phosphate, hanging drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 18% (w/v) PEG3350
structure of the unliganded F178Y mutant is determined to 1.4 A resolution
in complex with arabinose 5-phosphate, hanging drop vapor diffusion method, using 0.2 M K/Na tartrate and 20% (w/v) PEG 3350
hanging drop method, mother liquid consits of: 27% w/v polyethylenglycol 4000, 0.08% NaN3, 0.3 M ammonium acetate, pH 4.4
-
hanging-drop vapor diffusion method
crystallization in polymorphic forms, to 3.0 and 2.7 A resolution. Crystal form 1 belong to the orthorhombic space group C2221 with five monomers per asymmetric unit and crystal form 2 belong to the monoclinic space group P21 with ten monomers per asymmetric unit
-
mutant enzyme E60Q/F132Y, hanging drop vapor diffusion method, using 200 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol