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2.2.1.6: acetolactate synthase

This is an abbreviated version!
For detailed information about acetolactate synthase, go to the full flat file.

Word Map on EC 2.2.1.6

Reaction

2 pyruvate =

2-acetolactate
+
CO2

Synonyms

aceto-hydroxy acid synthase, acetohydroxy acid synthase, acetohydroxy acid synthase I, acetohydroxy acid synthetase, acetohydroxyacid synthase, acetolactate pyruvate-lyase (carboxylating), acetolactate synthase, acetolactate synthetase, acetolactate-synthase, acetolactic synthetase, AHAS, AHAS II, AHAS1, AHAS2, AHAS3, AHASL, AHASS, alpha-acetohydroxy acid synthetase, alpha-acetohydroxyacid synthase, alpha-acetolactate synthase, alpha-acetolactate synthetase, alpha-ALS, ALS, ALS1, ALS1R, ALS2, AlsS, CalS, catabolic acetolactate synthase, EC 4.1.3.18, GST-mALS, GST-wALS, Ilv2, Ilv6, ilvB, ilvB1, ilvB2, ilvG, IlvN, ilvX, Moilv2, Moilv6, More, PF0935, sll1981, synthase, acetolactate, TM_0548, TM_0549, TTHA1213

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.6 acetolactate synthase

Specific Activity

Specific Activity on EC 2.2.1.6 - acetolactate synthase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0023
pH 7.5, 37°C, purified recombinant His6-tagged mutant E85A
0.0043
-
substrate: pyruvate, pH 7.5, 37°C, in the presence of 3.5 M KCl
0.03
-
mutant H351Q, 37°C, pH 7.5
0.034
pH 7.5, 37°C, purified recombinant His6-tagged mutant E85Q
0.06
-
catalytic subunit ilvI, regulatory subunit ilvN, 37°C, pH 7.6
0.063
0.068
-
mutant DELTA630, 37°C, pH 7.4
0.09
-
mutant D374A/D375A, pH 7.5, 37°C
0.15
-
purified enzyme, at pH 7.4 and 37°C
0.17
isolated catalytic subunit, pH 7.5, 37°C
0.19
pH 7.5, 37°C, purified recombinant His6-tagged mutant H84T
0.22
pH 7.5, 37°C, purified recombinant His6-tagged mutant Q86A
0.64
-
mutant D374E/D375E, pH 7.5, 37°C
0.65
-
mutant D375A, pH 7.5, 37°C
0.66
-
-
0.77
-
mutant D374E, pH 7.5, 37°C
0.91
-
mutant D374A, pH 7.5, 37°C
1.02
-
mutant K255F, pH 7.5, 37°C
1.3
-
catalytic subunit ilvB, regulatory subunit ilvB, 37°C, pH 7.6
1.53
-
mutant D375E, pH 7.5, 37°C
1.55
-
wild-type, pH 7.5, 37°C
1.58
-
mutantH351F, 37°C, pH 7.5
1.6
-
mutant E47A, 37°C, pH 7.6
1.73
-
wild-type, 37°C, pH 7.4
1.87
-
wild-type, pH 7.5, 37°C
11.9
-
mutant W464L with His-tag, pH 7.6, 37°C
12
-
mutant enzyme A122V
12.6
wild-type, pH 7.0, 60°C
13.5
pH 7.5, 37°C
150
-
30°C, 60 ng protein per assay
17
-
wild-type with His-tag, pH 7.6, 37°C
2
-
mutant E47Q, 37°C, pH 7.6
2.17
-
mutant K255Q, pH 7.5, 37°C
2.6
-
mutant M250A with His-tag, pH 7.6, 37°C
20.9
-
wild-type AHAS III, truncated regulatory subunit ilvH-DELTA 86, pH 7.6, 37°C
22.7
-
mutant V375I, pH 7.6, 37°C
267
-
30°C, 90 ng protein per assay
2883
-
pH 7.0, 30°C, sulfonylurea-susceptible biotype
2950
-
pH 7.0, 30°C, sulfonylurea-resistant biotype
3.12
large subunit, pH 7.9, 55°C
3.23
-
mutant H392M, 37°C, pH 7.5
3.4
-
mutant F109M, pH 7.6, 37°C
32
-
catalytic subunit ilvB, truncated regulatory subunit ilvB-DELTA86, 37°C, pH 7.6
34.3
-
wild-type, pH 7.6, 37°C
35.4
-
at pH 6.5 and 37°C
36
presence of 20 mM acetate, pH 7.5, 37°C
4.15
-
mutant K299Q pH 7.5, 37°C
4.4
-
mutant enzyme W574L
4.6
-
purified holoenzyme
49.8
-
catalytic subunit ilvB, regulatory subunit ilvM, 37°C, pH 7.6
5.4
crude extract, at pH 7.0 and 80°C
5.78
-
wild-type, 37°C, pH 7.5
50.8
-
wild-type AHAS I, 37°C, pH 7.6
53
-
fusion protein with N-terminal oligohistidine at the large subunit
6.8
-
catalytic subunit, pH 7.0, 30°C
60.4
-
wild-type AHAS II, 37°C, pH 7.6
60.5
-
reconstituted, recombinant holoenzyme
63.6
-
recombinant holoenzyme
7.96
mutant Y35N/K139R/V172A/H474R, pH 7.0, 60°C
8.13
mutant H747R, pH 7.0, 60°C
8.2
-
mutant V375A, pH 7.6, 37°C
8.51
-
mutant S653N
9.16
holoenzyme, pH 7.9, 55°C
9.3
-
catalytic subunit ilvI, regulatory subunit ilvM, 37°C, pH 7.6
9.4
-
wild-type AHAS III, pH 7.6, 37°C
additional information