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2.2.1.6: acetolactate synthase

This is an abbreviated version!
For detailed information about acetolactate synthase, go to the full flat file.

Word Map on EC 2.2.1.6

Reaction

2 pyruvate =

2-acetolactate
+
CO2

Synonyms

aceto-hydroxy acid synthase, acetohydroxy acid synthase, acetohydroxy acid synthase I, acetohydroxy acid synthetase, acetohydroxyacid synthase, acetolactate pyruvate-lyase (carboxylating), acetolactate synthase, acetolactate synthetase, acetolactate-synthase, acetolactic synthetase, AHAS, AHAS II, AHAS1, AHAS2, AHAS3, AHASL, AHASS, alpha-acetohydroxy acid synthetase, alpha-acetohydroxyacid synthase, alpha-acetolactate synthase, alpha-acetolactate synthetase, alpha-ALS, ALS, ALS1, ALS1R, ALS2, AlsS, CalS, catabolic acetolactate synthase, EC 4.1.3.18, GST-mALS, GST-wALS, Ilv2, Ilv6, ilvB, ilvB1, ilvB2, ilvG, IlvN, ilvX, Moilv2, Moilv6, More, PF0935, sll1981, synthase, acetolactate, TM_0548, TM_0549, TTHA1213

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.6 acetolactate synthase

Storage Stability

Storage Stability on EC 2.2.1.6 - acetolactate synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -80°C, not stable
-
-20°C, 20% glycerol, stable for 2 months
-
-20°C, 50 mM phosphate, pH 8, 50% v/v glycerol, 3 weeks, less than 25% loss of activity
-
-20°C, stable for over 1 month
-
-25°C, no significant loss of activity after 6 months
-
-70°C, 21 days, 15% loss of activity of mutant enzyme A122V, less than 1% loss of activity of mutant enzymes W574S and S653N, 3% loss of activity of mutant enzyme W574L
-
-70°C, 3 weeks, loss of 5% of activity
-70°C, 4% loss of activity after 3 weeks with three freeze-thaw cycles, 8% loss of activity after 5 weeks with five freeze-thaw cycles, 9-10% loss of activity after 6 months with no intervening freeze-thaw cycle
-
-80°C, concentrated enzyme solution is indefinitely stable
-
0-4°C, 50 mM potassium phosphate, pH 7.0, 10 mM mercaptoethanol, 1 mM MgCl2, 0.01 mM thiamine diphosphate, 1 month, 20% loss of activity
-
0°C, purified recombinant enzyme, 5 days, 35-40% loss of activity
4°C, 10% loss of activity after 3 weeks, 23% loss of activity after 5 weeks
-
4°C, 21 days, 30% loss of activity of mutant enzyme A122V, 6% loss of activity of mutant enzyme W574S, 20% loss of activity of mutant enzyme W574L, 18% loss of activity of mutant enzyme S653N
-
4°C, 24 h, 36% loss of activity
-
4°C, 3 weeks, loss of 10% of activity
4°C, stable for several days
-