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2.2.1.7: 1-deoxy-D-xylulose-5-phosphate synthase

This is an abbreviated version!
For detailed information about 1-deoxy-D-xylulose-5-phosphate synthase, go to the full flat file.

Word Map on EC 2.2.1.7

Reaction

pyruvate
+
D-glyceraldehyde 3-phosphate
=
1-deoxy-D-xylulose 5-phosphate
 +
CO2

Synonyms

1-D-deoxy-D-threo-2-pentulose 5-phosphate synthetase, 1-D-deoxyxylulose 5-phosphate synthase, 1-deoxy-D-threo-pentulose synthase, 1-deoxy-D-xylulose 5-phosphate synthase, 1-deoxy-D-xylulose 5-phosphate synthase 1, 1-deoxy-D-xylulose 5-phosphate synthase 2, 1-deoxy-D-xylulose 5-phosphate synthetase, 1-deoxy-D-xylulose-5-phosphate pyruvate lyase, 1-deoxy-D-xylulose-5-phosphate synthase, 1-deoxy-D-xylulose-5-phosphate synthase 1, 1-deoxy-D-xylulose-5-phosphate synthetase, 1-desoxy-D-xylulose-5-phosphate synthase, CSDXS, D-1-deoxyxylulose 5-phosphate synthase, deoxyxylulose 5-phosphate synthase, deoxyxylulose-5-phosphate synthase, deoxyxylulose-5-phosphate synthetase, DXP, DXP synthase, DXP-synthase, DXPase, DXPS, DXS, DXS-I, DXS-II, DXS1, dxs11, DXS2, DXS3, EC 4.1.3.37, GbDXS1, GbDXS2, glyceraldehydes 3-phosphate-pyruvate ligase, GmDXS1, MtDXS1, MtDXS2, OsDXS1, OsDXS2, OsDXS3, PaDXS1, PaDXS2A, PaDXS2B, PdDXS1, PdDXS2, synthase, 1-deoxy-D-xylulose 5-phosphate

ECTree

     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase

Cloned

Cloned on EC 2.2.1.7 - 1-deoxy-D-xylulose-5-phosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in Escherichia coli
complementation assay in Escherichia coli
-
expressed in Arabidopsis thaliana protoplasts
expressed in Escherichia coli
expressed in Escherichia coli AB4-2 cells
-
expressed in Escherichia coli BL-21 B (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli strain EcAB4-2
-
expressed in Saccharomyces cerevisiae AH109 and Escherichia coli BL21 cells
expressed in transgenic hairy root lines of Salvia miltiorrhiza
expression in Escherichia coli
expression in Escherichia coli strain DH5alpha
expression in Morinda citrifolia
-
expression in Nicotiana tabacum
-
expression of MtDXS1 and MtDXS2 in Escherichia coli
-
gene dxs, DNA and amino acid sequence determination and analysis, expression profile analysis, phylogenetic analysis, expression in Escherichia coli strain DH5alpha
gene dxs, overexpression in transgenic Solanum tuberosum tubers
-
gene dxs1, semiquantitative expression analysis, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene dxs11, DNA and amino acid sequence determination and analysis, functional overexpression of the soluble enzyme in Escherichia coli, functional overexpression in Agrobacterium tumefaciens strain KCCM 10413, the transformed strain shows increased enzyme activity
gene dxs2, cloning from embryonic roots, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in and complementation of an enzyme-deficient Escherichia coli mutant strain
genes dxs1 and dxs2, semiquantitative expression analysis, DNA and amino acid sequence determination and analysis, phylogenetic analysis
genes dxs1, dxs2, and dxs3, DNA and amino acid sequence determination and analysis, phylogenetic tree
-
isolation of cDNA, expression analysis indicates developmental and organ-specific regulation of mRNA accumulation
-
mutant enzymes are expressed in Escherichia coli BL21(DE3) cells
-
overexpression
overexpression in Nicotiana tabacum
-
overexpression in transgenic Lavandula latifolia plants using the Agrobacterium tumefaciens transfection method
overexpression of the His-tagged enzyme in Escherichia coli, production of the selenomethionine enzyme form in Escherichia coli strain B834
transfection to Arabidopsis thaliana, expression in Escherichia coli mutants
two genes dxsA and dxsB, dxsA is located in the photosynthesis cluster, both isoenzymes expressed in Escherichia coli
-
use of a step wise deletion approach for the successful production of a catalytically active and soluble form of Plasmodium vivax 1-deoxy-D-xylulose-5-phosphate synthase in Escherichia coli. Signal and transit peptide removed soluble and functionally active enzyme is expressed in Escherichia coli