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2.3.1.1: amino-acid N-acetyltransferase

This is an abbreviated version!
For detailed information about amino-acid N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.1

Reaction

acetyl-CoA
+
L-glutamate
=
CoA
 +
N-acetyl-L-glutamate

Synonyms

acetylglutamate synthase, acetylglutamate synthetase, acetylglutamic synthetase, acetyltransferase, amino acid, AGAS, amino acid acetyltransferase, ARG2, ArgA, ArgH(A), argJ, Cg3035, More, N-acetyl-glutamate synthase, N-acetyl-L-glutamate synthase, N-acetyl-L-glutamate synthase/kinase, N-acetyl-L-glutamate synthetase, N-acetylglutamate synthase, N-acetylglutamate synthase/kinase, N-acetylglutamate synthetase, NAGS, NAGS-K, NAGS/K, NAT, ngNAGS, PaNAGS, pitax, Rv2747, SINAGS1, XcNAGS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.1 amino-acid N-acetyltransferase

Purification

Purification on EC 2.3.1.1 - amino-acid N-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE Biogel, Sephacryl S-200, aminoacetyl biogel A, affi-gel blue, sucrose gradient, isoelectric focusing
-
ammonium sulfate, hydroxyapatite
-
ammonium sulfate, hydroxyapatite, 10fold purification
-
ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephacryl-300
-
ammonium sulfate, hydroxyapatite, DEAE-cellulose, Sephadex G-100
-
DEAE-cellulose, hydroxylapatite
-
HisTrap nickel affinity column chromatography
-
HiTrap column chromatography
nickel affinity and DEAE columns
nickel affinity and Histrap column chromatography
nickel affinity column chromatography and DEAE column chromatography
recombinant enzyme, Ni2+-affinity column
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, ammonium sulfate fractionation, gel filtration, and ultrafiltration
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography, the His-tag is cleaved by thrombin
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography, the His-tag is ceaved by thrombin
recombinant His-tagged wild-type enzyme by nickel affinity chromatography
-
using Ni-NTA chromatography