2.3.1.129: acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase

This is an abbreviated version!
For detailed information about acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase, go to the full flat file.

Reaction

Synonyms

acyltransferase, uridine diphosphoacetylglucosamine, LiLpxA, LpxA, type II ACP-dependent UDP-N-acetylglucosamine acyltransferase, type II acyl carrier protein-dependent UDP-N-acetylglucosamine acyltransferase, UDP-N-acetylglucosamine 3-O-acyltransferase, UDP-N-acetylglucosamine acyltransferase, uridine diphosphoacetylglucosamine acyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.129 acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase

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Results	in table
19502	AA Sequence
5	Application
1	CAS Registry Number
23	Cloned(Commentary)
14	Crystallization (Commentary)
13	General Information
2	General Stability
17	IC50 Value
41	Inhibitors
3	Ki Value [mM]
9	KM Value [mM]
3	Localization
9	Metals/Ions
10	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
83	Organism
10	Pathway
236	PDB ID
13	pH Optimum
64	Protein Variants
6	Purification (Commentary)
4	Reaction
1	Reaction Type
65	Reference
1	Source Tissue
63	Specific Activity [micromol/min/mg]
2	Storage Stability
129	Substrates and Products (Substrate)
16	Subunits
48	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Stability [°C]
3	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.3.1.129 - acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

(3R)-3-hydroxylauroyl-methylphosphopantetheine + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

methylphosphopantetheine + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Leptospira interrogans

Q8EZA6

synthetic (3R)-3-hydroxylauroyl-methylphosphopantetheine is an excellent alternative donor substrate for LiLpxA

702322

-

-

?

(R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxydecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Mesorhizobium loti

-

-

659349

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

3 entries

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-galactosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-galactosamine

2 entries

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

4 entries

(R)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

659349

-

-

?

(R)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-((3R)-3-hydroxylauroyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Leptospira interrogans

Q8EZA6

LiLpxA is highly selective for (R)-3-hydroxylauroyl-[acyl-carrier protein] and UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

702322

-

-

?

(R)-3-hydroxymyristoyl-[acyl carrier protein] + UDP-N-acetylglucosamine

[acyl-carrier protein] + UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine

Escherichia coli

P0A722

first step in lipid A biosynthesis

675443

-

-

?

(R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxymyristoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

first step in lipid A biosynthesis

689754

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

5 entries

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

29 entries

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-butyrylglucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-butyrylglucosamine

Escherichia coli

-

transacylation at 8% the rate of the reaction with UDP-N-acetylglucosamine

486251

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-propionyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-propionyl-alpha-D-glucosamine

Escherichia coli

-

transacylation at 22% the rate of the reaction with UDP-N-acetylglucosamine

486251

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

12 entries

(R,S)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine

2 entries

(R,S)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxylauroyl)-N-acetyl-alpha-D-glucosamine

3 entries

(R,S)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxymyristoyl)-N-acetyl-alpha-D-glucosamine

3 entries

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

20 entries

(S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

transacylation at 7% the rate of (R)-enantiomer

486251

-

-

?

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

5 entries

decanoyl-[acyl-carrier protein] + UDP-N-acetylglucosamine

[acyl-carrier protein] + UDP-3-O-decanoyl-N-acetylglucosamine

Pseudomonas aeruginosa

-

-

486261

-

-

?

myristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-myristoyl-N-acetyl-alpha-D-glucosamine

Chlamydia trachomatis

-

higher specific activity than to (R,S)-3-hydroxymyristoyl-[acyl-carrier-protein]

486263

-

-

?

R,S-3-hydroxypalmitoyl-ACP + UDP-N-acetyl-alpha-D-glucosamine

ACP + UDP-3-O-(3-hydroxypalmitoyl)-N-acetyl-alpha-D-glucosamine

Francisella tularensis subsp. novicida

-

-

755940

-

-

?

R-3-hydroxydecanoyl-[acyl carrier protein] + UDP-N-acetylglucosamine

[acyl-carrier protein] + UDP-3-O-hydroxydecanoyl-N-acetylglucosamine

Escherichia coli

P0A722

first step in lipid A biosynthesis

689754

-

-

?

additional information

?

-

13 entries

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Leptospira interrogans

-

first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units

659349

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Leptospira interrogans

-

the enzyme is absolute specific for 3-hydroxylauroyl-ACP as donor substrate

659349

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Mesorhizobium loti

-

-

659349

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-galactosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-galactosamine

Moraxella catarrhalis

D5VAW8

-

756971

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-galactosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-galactosamine

Moraxella catarrhalis BBH18

D5VAW8

-

756971

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

659349

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

-

758450

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Moraxella catarrhalis

D5VAW8

-

756971

-

-

?

(R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Moraxella catarrhalis BBH18

D5VAW8

-

756971

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Acidithiobacillus ferrooxidans

Q6QHI7

first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Acidithiobacillus ferrooxidans

Q6QHI7

specific for UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Escherichia coli

-

only in vitro

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Mesorhizobium loti

-

-

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

[acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose

Mesorhizobium loti

-

first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Acidithiobacillus ferrooxidans

Q6QHI7

low activity

659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Acinetobacter calcoaceticus

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella bronchiseptica

-

-

486262

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella pertussis

-

-

486262

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Cereibacter sphaeroides

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Citrobacter freundii

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486254, 486259, 659349

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

essential for cell growth of gram-negative bacteria

486251

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

involved with EC 2.4.1.182 and 2.7.1.130 in the biosynthesis of the phosphorylated glycolipid and outer membrane component, Lipid A

486251, 486256

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

initial reaction in lipid A biosynthesis

486251, 486252

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

LpxA is essential for the growth of Escherichia coli, and is important in lipid A biosynthesis, overview

676803

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

the enzyme is responsible for the first step of lipid A biosynthesis, lipid A is an integral component of the lipopolysaccharide that forms the selective and protective outer monolayer of Gram-negative bacteria, and is essential for bacterial growth and viability

675443

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

i.e. (R)-3-hydroxymyristoyl-[acyl-carrier protein], the enzyme catalyzes the first step of lipid A biosynthesis, the transfer of the (R)-3-hydroxyacyl chain from (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] to the glucosamine 3-OH group of UDP-GlcNAc

676803

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

substrate binding site structure with prominent hydrophilic interactions between highly conserved clusters of residues Asn198, Glu200, Arg204 and Arg205 with UDP, and Asp74, His125, His144 and Gln161 with the GlcNAc moiety, these interactions serve to bind and orient the substrate for catalysis, overview

675443

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

P0A722

substrate binding site structure with prominent hydrophilic interactions between highly conserved clusters of residues Asn198, Glu200, Arg204 and Arg205 with UDP, and Asp74, His125, His144 and Gln161 with the GlcNAc moiety, these interactions serve to bind and orientate the substrate for catalysis, overview

675443

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli JB1104

-

initial reaction in lipid A biosynthesis

486252

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli JB1104

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli SM105

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli W3106

-

initial reaction in lipid A biosynthesis

486252

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Helicobacter pylori

-

-

660471

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Helicobacter pylori

-

first step in lipid A biosynthesis pathway

660471

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Klebsiella aerogenes

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Klebsiella oxytoca

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Neisseria meningitidis

-

-

486259

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Proteus mirabilis

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Pseudomonas aeruginosa

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Serratia marcescens

-

-

486254

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Vibrio harveyi

-

-

674883

-

-

?

(R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Vibrio harveyi

-

i.e. (R)-3-hydroxymyristoyl-[acyl-carrier protein], the forward reaction direction is thermodynamically unfavorable, recombinant His-tagged enzyme, the activity with mutant acyl-carrier proteins is impaired, especially with mutations of the B-site of the acyl-carrier protein, recombinant wild-type and mutant ACP substrates, overview

674883

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Acinetobacter calcoaceticus

-

main activity in crude extracts

486254

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella bronchiseptica

-

-

486262

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella parapertussis

-

-

486262

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella pertussis

-

-

486262

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Cereibacter sphaeroides

-

-

486254

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486254, 486259, 486260

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

i.e. 3-hydroxylauroyl-[acyl-carrier-protein], poor substrate

486251

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli JB1104

-

-

486254

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli SM105

-

-

486254

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Neisseria meningitidis

-

-

486259

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Pseudomonas aeruginosa

-

-

486260

-

-

?

(R,S)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine

Pseudomonas aeruginosa

-

main activity in crude extracts

486254

-

-

?

(R,S)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella bronchiseptica

-

-

486262

-

-

?

(R,S)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella pertussis

-

-

486262

-

-

?

(R,S)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxylauroyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486259

-

-

?

(R,S)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxylauroyl)-N-acetyl-alpha-D-glucosamine

Neisseria meningitidis

-

-

486259

-

-

?

(R,S)-3-hydroxylauroyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxylauroyl)-N-acetyl-alpha-D-glucosamine

Pseudomonas aeruginosa

-

-

486261

-

-

?

(R,S)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxymyristoyl)-N-acetyl-alpha-D-glucosamine

Chlamydia trachomatis

-

-

486263

-

-

?

(R,S)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxymyristoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486259

-

-

?

(R,S)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxymyristoyl)-N-acetyl-alpha-D-glucosamine

Neisseria meningitidis

-

-

486259

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Acinetobacter calcoaceticus

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella bronchiseptica

-

-

486262

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Bordetella pertussis

-

-

486262

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Cereibacter sphaeroides

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Citrobacter freundii

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486252, 486254, 486256, 486257, 486259, 486260

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

486255, 486258

-

-

r

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein]

486250, 486251

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein]

486253

-

-

r

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

high specificity with respect to acyl-donor, equilibrium constant favors thioester acyl carrier substrate

486253

-

-

r

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli JB1104

-

no substrates are UDPglucosamine, UDP-N-(R)-3-hydroxymyristoylglucosamine, ADP-N-acetylglucosamine, GDP-N-acetylglucosamine, CDP-N-acetylglucosamine, (R)-3-hydroxytetradecanoyl-CoA, or palmitoyl-CoA, myristoyl-[acyl-carrier-protein], and palmitoyl-[acyl-carrier-protein]

486250

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli JB1104

-

-

486252, 486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli SM105

-

-

486254, 486257

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli W3106

-

-

486252

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Klebsiella aerogenes

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Klebsiella oxytoca

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Neisseria meningitidis

-

-

486259

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Proteus mirabilis

-

-

486254

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Pseudomonas aeruginosa

-

-

486254, 486260, 486261

-

-

?

(R,S)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

[acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Serratia marcescens

-

-

486254

-

-

?

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Arabidopsis thaliana

Q9SU91

-

718930, 720913

-

-

r

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Arabidopsis thaliana

Q9SU91

specificity for (R)-3-hydroxymyristoyl-ACP over other chain lengths in vitro

718930

-

-

r

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

718571

-

-

?

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

-

720069

-

-

r

an (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine

an [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine

Escherichia coli

-

assay is optimal at 10 nM LpxA

718571

-

-

?

additional information

?

-

Acidithiobacillus ferrooxidans

Q6QHI7

substrate specificity, lipid A structure overview

659349

-

-

?

additional information

?

-

Escherichia coli

-

substrate specificity, no activity with UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose due to lack of genes gnnA and gnnB, lipid A structure overview

659349

-

-

?

additional information

?

-

Escherichia coli

P0A722

nucleotide substrate recognition by LpxA, overview

675443

-

-

?

additional information

?

-

Escherichia coli

-

nucleotide substrate recognition by LpxA, overview

675443

-

-

?

additional information

?

-

Escherichia coli

-

development of a fluorescence polarization assay, detecting displacement of a fluorescently labeled peptide inhibitor based on the previously reported inhibitor peptide 920 by active site ligands, for high-throughput screening of LpxA competitve inhibitors, overview

720069

-

-

?

additional information

?

-

Leptospira interrogans

-

substrate specificity, no activity with UDP-N-acetylglucosamine, lipid A structure overview

659349

-

-

?

additional information

?

-

Mesorhizobium loti

-

substrate specificity, no activity with UDP-N-acetylglucosamine, equally effective with 3-hydroxyacyl-ACP of different chain lengths, lipid A structure overview

659349

-

-

?

additional information

?

-

Moraxella catarrhalis

Q3BDJ0

the enzyme is responsible for the first step of lipid A biosynthesis

673981

-

-

?

additional information

?

-

Moraxella catarrhalis

-

the enzyme is responsible for the first step of lipid A biosynthesis

673981

-

-

?

additional information

?

-

Moraxella catarrhalis

D5VAW8

LpxA displays better binding affinity with UDP-GlcNAc than with UDP-GalNAc. Binding of UDP-GlcNActo LpxA is driven by a very favorable enthalpy change(DeltaH = ?6.03 kcal/mol) with a dissociation constant of 22.62 microM. One monomer of McLpxA binds to one molecule ofUDP-GlcNAc

756971

-

-

-

additional information

?

-

Moraxella catarrhalis

-

LpxA displays better binding affinity with UDP-GlcNAc than with UDP-GalNAc. Binding of UDP-GlcNActo LpxA is driven by a very favorable enthalpy change(DeltaH = ?6.03 kcal/mol) with a dissociation constant of 22.62 microM. One monomer of McLpxA binds to one molecule ofUDP-GlcNAc

756971

-

-

-

additional information

?

-

Moraxella catarrhalis BBH18

D5VAW8

LpxA displays better binding affinity with UDP-GlcNAc than with UDP-GalNAc. Binding of UDP-GlcNActo LpxA is driven by a very favorable enthalpy change(DeltaH = ?6.03 kcal/mol) with a dissociation constant of 22.62 microM. One monomer of McLpxA binds to one molecule ofUDP-GlcNAc

756971

-

-

-

additional information

?

-

Moraxella catarrhalis O35E

Q3BDJ0

the enzyme is responsible for the first step of lipid A biosynthesis

673981

-

-

?