2.3.1.135: phosphatidylcholine-retinol O-acyltransferase
This is an abbreviated version!
For detailed information about phosphatidylcholine-retinol O-acyltransferase, go to the full flat file.
Word Map on EC 2.3.1.135
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2.3.1.135
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retinoids
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retinal
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all-trans-retinyl
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opsin
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11-cis-retinol
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isomerohydrolase
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amaurosis
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leber
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s-opsins
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11-cis-retinoids
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medicine
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crbp-i
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diagnostics
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analysis
- 2.3.1.135
-
retinoids
- retinal
-
all-trans-retinyl
- opsin
- 11-cis-retinol
-
isomerohydrolase
- amaurosis
-
leber
-
s-opsins
-
11-cis-retinoids
- medicine
-
crbp-i
- diagnostics
- analysis
Reaction
Synonyms
11-cis-acyl-CoA:retinol O-acyltransferase, acyltransferase, lecithin-retinol, EC 5.2.1.3, lecithin retinol acyl transferase, lecithin retinol acyltransferase, lecithin-retinol acyltransferase, lecithin/retinol acyltransferase, lecithin: retinol acyltransferase, lecithin:retinol acyl transferase, lecithin:retinol acyltransferase, LRAT, More, retinyl ester synthase, retinyl-ester synthase
ECTree
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Cloned
Cloned on EC 2.3.1.135 - phosphatidylcholine-retinol O-acyltransferase
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DNA sequence determination and analysis of the full length gene including the 3'- and 5'-ends, genetic organization analysis, at least 2 splicing variants, the second of which lacks a 103 nt polynucleotide in the 5'-UTR of the full length transcript
expression in HEK-293T cells
expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents
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expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3)
expression of wild-type and/or mutant enzymes inducibly in HEK-293 cells, transiently in COS-7 cells, and in Escherichia coli strain BL21(DE3) cells
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inducible expression of LRAT in HEK-293S cells
LRAT, quantitative real-time RT-PCR expression analysis in melanoma cell lines
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stable expression in HEK-293T cells
stable expression of GFP-tagged LRAT in CHO cells and of GFP-tagged LRAT in COS7 cells
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