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2.3.1.135: phosphatidylcholine-retinol O-acyltransferase

This is an abbreviated version!
For detailed information about phosphatidylcholine-retinol O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.135

Reaction

phosphatidylcholine
+
retinol-[cellular-retinol-binding-protein]
=
2-acylglycerophosphocholine
+
retinyl-ester-[cellular-retinol-binding-protein]

Synonyms

11-cis-acyl-CoA:retinol O-acyltransferase, acyltransferase, lecithin-retinol, EC 5.2.1.3, lecithin retinol acyl transferase, lecithin retinol acyltransferase, lecithin-retinol acyltransferase, lecithin/retinol acyltransferase, lecithin: retinol acyltransferase, lecithin:retinol acyl transferase, lecithin:retinol acyltransferase, LRAT, More, retinyl ester synthase, retinyl-ester synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.135 phosphatidylcholine-retinol O-acyltransferase

Cloned

Cloned on EC 2.3.1.135 - phosphatidylcholine-retinol O-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis of the full length gene including the 3'- and 5'-ends, genetic organization analysis, at least 2 splicing variants, the second of which lacks a 103 nt polynucleotide in the 5'-UTR of the full length transcript
expression in HEK-293 cells
expression in HEK-293T cells
expression in NIH-3T3 cell
expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents
-
expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3)
expression of wild-type and/or mutant enzymes inducibly in HEK-293 cells, transiently in COS-7 cells, and in Escherichia coli strain BL21(DE3) cells
-
gene lrat is localized on chromosome 4 at locus 4q31.2
-
gene Lrat, vector cloning for construction of knockout mutants
-
inducible expression of LRAT in HEK-293S cells
LRAT, quantitative real-time RT-PCR expression analysis in melanoma cell lines
-
recombinant expression of His-tagged LRAT
-
recombinant expression of the truncated tLRAT and its S175R mutant
-
stable expression in HEK-293T cells
stable expression of GFP-tagged LRAT in CHO cells and of GFP-tagged LRAT in COS7 cells
-
stable expression of LRAT in HEK-293T cell membranes
-