2.3.1.135: phosphatidylcholine-retinol O-acyltransferase
This is an abbreviated version!
For detailed information about phosphatidylcholine-retinol O-acyltransferase, go to the full flat file.
Word Map on EC 2.3.1.135
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2.3.1.135
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retinoids
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retinal
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all-trans-retinyl
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opsin
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11-cis-retinol
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isomerohydrolase
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amaurosis
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leber
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s-opsins
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11-cis-retinoids
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medicine
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crbp-i
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diagnostics
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analysis
- 2.3.1.135
-
retinoids
- retinal
-
all-trans-retinyl
- opsin
- 11-cis-retinol
-
isomerohydrolase
- amaurosis
-
leber
-
s-opsins
-
11-cis-retinoids
- medicine
-
crbp-i
- diagnostics
- analysis
Reaction
Synonyms
11-cis-acyl-CoA:retinol O-acyltransferase, acyltransferase, lecithin-retinol, EC 5.2.1.3, lecithin retinol acyl transferase, lecithin retinol acyltransferase, lecithin-retinol acyltransferase, lecithin/retinol acyltransferase, lecithin: retinol acyltransferase, lecithin:retinol acyl transferase, lecithin:retinol acyltransferase, LRAT, More, retinyl ester synthase, retinyl-ester synthase
ECTree
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KM Value
KM Value on EC 2.3.1.135 - phosphatidylcholine-retinol O-acyltransferase
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0.00024
all-trans-retinol-[cellular-retinol-binding-protein]
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pH and temperature not specified in the publication
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0.00024
retinol-(cellular-retinol-binding protein) type II
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intestinal LRAT, reaction with phosphatidylcholine
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0.0005
retinol-(cellular-retinol-binding-protein)
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reaction with phosphatidylcholine
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0.00032
retinol-[cellular-retinol-binding-proteintype II]
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liver LRAT, reaction with phosphatidylcholine
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0.0023
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pH 7.5, 37°C, retinal pigment epithelium microsomes
0.00036
all-trans-retinol
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with dipalmitoylphosphatidylcholine, cloned enzyme, expressed in HEK-293T cells
0.00046
all-trans-retinol
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with dipalmitoylphosphatidylcholine, chemically dimerized enzyme
0.00094
all-trans-retinol
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with dipalmitoylphosphatidylcholine, cloned enzyme, expressed in HEK-296T cells
0.00033
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pH 7.5, 37°C, retinal pigment epithelium microsomes
0.0002
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intestinal LRAT, reaction with phosphatidylcholine
0.00078
retinol-[cellular-retinol-binding-protein]
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liver LRAT, reaction with phosphatidylcholine
0.001
retinol-[cellular-retinol-binding-protein]
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liver LRAT, reaction with phosphatidylcholine
additional information
additional information
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steady-state kinetic analysis using purified truncated mutant tLRAT
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additional information
additional information
the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs
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