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2.3.1.142: glycoprotein O-fatty-acyltransferase

This is an abbreviated version!
For detailed information about glycoprotein O-fatty-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.142

Reaction

palmitoyl-CoA
+
mucus glycoprotein
=
CoA
 +
O-palmitoylglycoprotein

Synonyms

acyltransferase, protein, CRTAase, GlnA1, LPCAT1, MTAase, Pat, PATI, protein acetyltransferase, protein fatty acyltransferase, ras palmitoyl acyltransferase, Ras PAT

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.142 glycoprotein O-fatty-acyltransferase

Engineering

Engineering on EC 2.3.1.142 - glycoprotein O-fatty-acyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A140G
-
changed selectivity of acyltransferase for the 2 acetylation sites of adenylate cyclase toxin, a mixture of bi and monoacetylated toxins modified either at both Lys860 and Lys983 or only at Lys860 is generated
A140V
-
monoacetylation of adenylate cyclase toxin almost exclusively at Lys983
C67S
-
no effect on adenylate cyclase toxin activation
H33D
-
loss of adenlayte cyclase toxin acetylation activity
H33S
-
loss of adenlayte cyclase toxin acetylation activity
R141K
-
no effect on adenylate cyclase toxin activation
R141L
-
no effect on adenylate cyclase toxin activation
S30R
-
loss of adenlayte cyclase toxin acetylation activity
S30W
-
loss of adenlayte cyclase toxin acetylation activity
S68T
-
no effect on adenylate cyclase toxin activation
additional information
-
construction of a fusion protein that places the 58 amino acids of Erf2 onto the C-terminus of Pfa4, another DHHC enzyme that resides in the endoplasmic reticulum membrane, the modification highly reduces the half-life of the engineered protein