2.3.1.160: vinorine synthase

This is an abbreviated version!
For detailed information about vinorine synthase, go to the full flat file.

Word Map on EC 2.3.1.160

2.3.1.160

rauvolfia

ajmaline

acyltransferases

serpentina

antiarrhythmic

indole

monoterpenoid

morphine

gardneral

hanging-drop

coa-dependent

hxxxd

vindoline

strictosidine

deacetylvindoline

Reaction

Synonyms

More, vinorine synthase, VS

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.160 vinorine synthase

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Results	in table
55	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
2	Crystallization (Commentary)
7	Inhibitors
14	KM Value [mM]
4	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
6	Organism
3	Pathway
2	PDB ID
4	pH Optimum
1	pI Value
18	Protein Variants
6	Purification (Commentary)
4	Reaction
1	Reaction Type
6	Reference
1	Source Tissue
2	Specific Activity [micromol/min/mg]
14	Substrates and Products (Substrate)
3	Subunits
11	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.3.1.160 - vinorine synthase

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purified detagged recombinant wild-type or selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 32°C, 2-3 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 mM acetyl-CoA, with reservoir solution containing 0.1 M Tris-HCl, pH 8.7, 2 M ammonium sulfate, 2% PEG 400, cryoprotection by 20-25% glycerol, X-ray diffraction structure determination anad analysis at 2.6 A resolution, modeling of CoA binding

Rauvolfia serpentina

Q70PR7

662350

using the hanging-drop vapour-diffusion method

Rauvolfia serpentina

682286