2.3.1.167: 10-deacetylbaccatin III 10-O-acetyltransferase

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For detailed information about 10-deacetylbaccatin III 10-O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.167

2.3.1.167

taxus

taxoids

endophytic

needle

baccata

yunnanensis

synthesis

taxol-producing

chinensis

cephalomannine

cuspidata

taxadiene

diterpenoids

10-deacetyltaxol

mairei

wallichiana

oxetane

twigs

pharmacology

biotechnology

Reaction

Synonyms

10-DAB, 10-DABT, 10-DBAT, 10-deacetylbaccatin III 10-O-acetyltransferase, 10-deacetylbaccatin III 10beta-O-acetyltransferase, 10-deacetylbaccatin III-10-beta-O-acetyltransferase, 10-deacetylbaccatin III-10-O-acetyl transferase, 10-deacetylbaccatin III-10-O-acetyltransferase, 10-deacetylbaccatin III-10beta-O-acetyltransferase, 10-deacetylbaccatin III:10beta-O-acetyltransferase, 10-deacetylbaccatin-III-10beta-O-acetyltransferase, 10-hydroxytaxane 10-O-acetyltransferase, 10-hydroxytaxane O-acetyltransferase, 10beta-O-acetyltransferase, acetyl CoA:10-deacetylbaccatin-III 10-O-acetyltransferase, acetyl coenzyme A:10-hydroxytaxane O-acetyltransferase, DBAT, taxoid 10beta-O-acetyl transferase, taxoid 10beta-O-acetyltransferase, TmDBAT

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.167 10-deacetylbaccatin III 10-O-acetyltransferase

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Results	in table
3	Activating Compound
28	AA Sequence
11	Application
1	CAS Registry Number
36	Cloned(Commentary)
2	Cofactor
3	Expression
5	General Information
1	General Stability
24	kcat/KM [mM/s]
32	KM Value [mM]
11	Localization
1	Metals/Ions
8	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
54	Organism
6	Pathway
7	pH Optimum
2	pH Range
1	pI Value
27	Protein Variants
10	Purification (Commentary)
1	Reaction
26	Reaction Type
50	Reference
22	Source Tissue
3	Specific Activity [micromol/min/mg]
58	Substrates and Products (Substrate)
2	Subunits
126	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
24	Turnover Number [1/s]

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Cloned on EC 2.3.1.167 - 10-deacetylbaccatin III 10-O-acetyltransferase

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Lasiodiplodia theobromae

A0A0D4BT92

756740

expression in Escherichia coli

6 entries

gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview

Taxus sp.

672726

into the TA cloning vector pMD19-T for sequencing

23 entries

into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells

Taxus sp.

672726

overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag

Taxus baccata

660432

the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells

Taxus x media

Q6SQJ5

688912