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2.3.1.178: diaminobutyrate acetyltransferase

This is an abbreviated version!
For detailed information about diaminobutyrate acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.178

Reaction

acetyl-CoA
+
L-2,4-diaminobutanoate
=
CoA
+
(2S)-4-acetamido-2-aminobutanoate

Synonyms

2,4-diaminobutanoate acetyltransferase, DAB acetyltransferase, DABA acetyltransferase, DABAAT, DABAcT, diaminobutyrate acetyltransferase, diaminobutyric acid acetyltransferase, EctA, EctC, L-2,4-diaminobutanoate acetyltransferase, L-2,4-diaminobutyrate acetyltransferase, L-diaminobutyric acid acetyl transferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.178 diaminobutyrate acetyltransferase

Cloned

Cloned on EC 2.3.1.178 - diaminobutyrate acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into the expression vector pQE30 with a 6×histidine tag and expressed in Escherichia coli JM109 M15 cells
MH020162
expression in Escherichia coli
-
expression in Escherichia coli with an additional six His residues at the C-terminus
-
expression in Escherichia coli, also osmoregulated in Escherichia coli
-
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
gene ectA, ectABC operon, in Marinococcus halophilus, the transcription of ectABC genes is initiated from three individual sigma70/sigmaA-promoters locate upstream of each gene
-
gene ectA, encoded in ectABC, transcription of the ectABC-ask operon is initiated from two sigma70-dependent promoters ectAp1 and ectAp2. Gene ectR1 encodes the MarR-like transcriptional regulator EctR that is involved in the regulation of ectABC, overview
-
heterologous production of EctA in Escherichia coli, with either a N-terminal (NH2-WSHPQFEK-SG) or a C-terminal (SA-WSHPQFEK-COOH) Strep-Tag II peptide for their purification by affinity chromatography
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
our putative promoters (?ectA1-4) are located upstream of the ectA gene and one internal promoter PectB is upstream of the ectB gene
-