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2.3.1.179: beta-ketoacyl-[acyl-carrier-protein] synthase II

This is an abbreviated version!
For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase II, go to the full flat file.

Word Map on EC 2.3.1.179

Reaction

a (Z)-hexadec-9-enoyl-[acyl-carrier protein]
+
a malonyl-[acyl-carrier protein]
=
a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein]
 +
CO2
 +
an [acyl-carrier protein]

Synonyms

3-ketoacyl acyl synthase II, 3-ketoacyl-ACP synthase, 3-ketoacyl-ACP synthase 2, 3-ketoacyl-ACP synthase II, 3-oxoacyl-(acylcarrier protein) synthase II, 3-oxoacyl-ACP synthase II, B-ketoacyl-ACP synthase II, beta-ketoacyl ACP-synthase II, beta-ketoacyl acyl carrier protein synthase II, beta-ketoacyl acyl-carrier protein synthase II, beta-ketoacyl synthase II, beta-ketoacyl-(acyl-carrier-protein) synthase II, beta-ketoacyl-ACP synthase FabF3, beta-ketoacyl-ACP synthase II, beta-ketoacyl-acyl carrier protein synthase I/II, beta-ketoacyl-acyl carrier protein synthase II, beta-ketoacyl-acyl carrier protein synthases II, beta-ketoacyl-acyl carrier protein synthetase II, beta-ketoacyl-acyl-carrier protein synthase II, beta-ketoacyl-acyl-carrier-protein synthase II, beta-ketoacyl-[ACP] synthase II, beta-ketoacyl-[ACP] synthase-II, beta-ketoacyl-[acyl carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier-protein] synthase II, FabB, FabB/F, FabF, FabF elongation condensing enzyme, FabF of type II fatty acid biosynthesis, FabF1, FASII, fatty acid synthesis type II, KAS II, KAS-II, KAS2, KASII

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.179 beta-ketoacyl-[acyl-carrier-protein] synthase II

Cloned

Cloned on EC 2.3.1.179 - beta-ketoacyl-[acyl-carrier-protein] synthase II

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtKAS2, DNA and amino acid sequence determination and analysis, sequence comparisons, expression pattern, the promoter is active in various tissues, also in the embryo
chimeric protein js200-FabF (the first 200 amino residues from the N-terminal of Streptomyces albus FabF and the last 200 residues from the C-terminal of Streptomyces platensis FabF are combined) is expressed in Escherichia coli BL21(DE3) cells
construction of plasmids pXspFabF(M1) and pXspFabF(M2). The resulting plasmids are transformed into Escherichia coli XL1-Blue competent cells. Subsequently, pXSpFabF(M1) and pXspFabF(M2) isolated from Escherichia coli XL1-Blue cells are transformed into the expression strain Escherichia coli BL21 (DE3)
determined with the multiple isomorphous replacement method and refined at 2.4 A resolution. Hanging drop vapor diffusion method at room temperature, using 27% PEG 8000 as precipitant, buffered at pH 7.5 with 0.1 M HEPES. The crystals grow to a size of 0.5 * 0.3 * 0.2 mm3 within 3 days. The lifetime of these crystals is very limited, they will dissolve within 10 days of their appearance. Addition of 0.1% mercaptoethanol to the reservoir solution significantly increases the life time of the crystals. Space group: P3(1)21 with cell dimensions a = 76.4 A, c = 146.8 A, gamma = 120°
expressed in Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3)
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli cells lacking FabF
expression in Escherichia coli
expression of wild-type and mutant His-tagged FabF in Escherichia coli strain BL21(DE3)
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gene fabF, recombinant expression of N-terminally His6-tagged enzyme with a TEV protease cleavage site in Escherichia coli strain BL21(DE3) pLysS
Q7CJ22
gene fabF, recombinant wild-type and mutant N-terminally His6-tagged enzyme expressions from vector pNIC28-Bsa4 including a TEV cleavage site in Escherichia coli strain BL21(DE3)pLysS
gene fabF, subcloning in Escherichia coli strain XL1-Blue, expression in Escherichia coli strain BL21(DE3)
gene fabF1, expression in Escherichia coli strain BL21(DE3), and in strain K1060, a strain that carries an unconditional fabB allele, and in Escherichia coli strain CY242, which carries the same fabB(Ts) allele as strain CY244, but fabF1 fails to complement growth of the temperature sensitive fabB mutant strain CY242 at 42°C
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gene KAS-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, cloning and expression in Escherichia coli strain DH5alpha
gene KAS2, bothKASII genes are simultaneously inhibited via three different RNAi constructs, quantitative real-time PCR enzyme expression analysis
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gene KAS2, transient expression in Nicotiana bethaminana, in which both KASII genes are simultaneously inhibited via three different RNAi constructs, using Agrobacterium tumefaciens transfection method
gene KASII, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, cloning and expression in Escherichia coli strain DH5alpha
gene PffabB/Fm, FabB/F is encoded at locus MAL6P1.165, cloning and expression, expression in and complementation of Escherichia coli strain CY244, which is deficient in FabB and FabF, the latter by mutations S220N and G262M
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His-tagged protein expressed in Escherichia coli BL21(DE3)
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His-tagged protein expressed in Escherichia coli is insoluble and not functional, GFP fusion protein expressed in Arabidopsis thaliana, Arabidopsis plants expressing GFP fusions have elevated levels of arachidic acid (C20:0) and erucic acid (C22:1) at the expense of stearic acid (C18:0) and oleic acid (C18:1)
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His-tagged protein expressed in Escherichia coli pLysS
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Lactococcus lactis FabF can functionally replace both the FabB (EC 2.3.1.41) and FabF (2.3.1.179) proteins of Escherichia coli and the FabH protein of Lactococcus lactis
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overexpressed in Enterococcus faecalis
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