2.3.1.179: beta-ketoacyl-[acyl-carrier-protein] synthase II

This is an abbreviated version!
For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase II, go to the full flat file.

Reaction

Synonyms

3-ketoacyl acyl synthase II, 3-ketoacyl-ACP synthase, 3-ketoacyl-ACP synthase 2, 3-ketoacyl-ACP synthase II, 3-oxoacyl-(acylcarrier protein) synthase II, 3-oxoacyl-ACP synthase II, B-ketoacyl-ACP synthase II, beta-ketoacyl ACP-synthase II, beta-ketoacyl acyl carrier protein synthase II, beta-ketoacyl acyl-carrier protein synthase II, beta-ketoacyl synthase II, beta-ketoacyl-(acyl-carrier-protein) synthase II, beta-ketoacyl-ACP synthase FabF3, beta-ketoacyl-ACP synthase II, beta-ketoacyl-acyl carrier protein synthase I/II, beta-ketoacyl-acyl carrier protein synthase II, beta-ketoacyl-acyl carrier protein synthases II, beta-ketoacyl-acyl carrier protein synthetase II, beta-ketoacyl-acyl-carrier protein synthase II, beta-ketoacyl-acyl-carrier-protein synthase II, beta-ketoacyl-[ACP] synthase II, beta-ketoacyl-[ACP] synthase-II, beta-ketoacyl-[acyl carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier-protein] synthase II, FabB, FabB/F, FabF, FabF elongation condensing enzyme, FabF of type II fatty acid biosynthesis, FabF1, FASII, fatty acid synthesis type II, KAS II, KAS-II, KAS2, KASII

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.179 beta-ketoacyl-[acyl-carrier-protein] synthase II

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Results	in table
43692	AA Sequence
9	Application
1	CAS Registry Number
27	Cloned(Commentary)
11	Crystallization (Commentary)
1	Expression
23	General Information
1	General Stability
7	IC50 Value
70	Inhibitors
8	kcat/KM [mM/s]
23	KM Value [mM]
2	Localization
1	Metals/Ions
9	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
62	Organism
26	Pathway
44	PDB ID
5	pH Optimum
3	pI Value
27	Protein Variants
12	Purification (Commentary)
4	Reaction
1	Reaction Type
57	Reference
30	Source Tissue
3	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
13	Subunits
104	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Engineering

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Engineering on EC 2.3.1.179 - beta-ketoacyl-[acyl-carrier-protein] synthase II

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

L337F

Arabidopsis thaliana

Q9C9P4

the point mutation, mutant fab1-1, causes a partially deficient KAS2 activity

703859

E122K

Chlamydia trachomatis

A0A0H3MGB3

the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides

755804

G200S

Chlamydia trachomatis

A0A0H3MGB3

the mutant is resistant towards inhibition by acylated sulfonamides

755804

E122K

Chlamydia trachomatis 434/Bu

-

the mutant is less inhibited by cerulenin and platensimycin compared to the wild type enzyme and is resistant towards inhibition by acylated sulfonamides

-

G200S

Chlamydia trachomatis 434/Bu

-

the mutant is resistant towards inhibition by acylated sulfonamides

-

C163Q

Escherichia coli

P0AAI5

site-directed mutagenesis, interaction with platensimycin compared to the interaction with the wild-type enzyme

676148

R206G

Escherichia coli

-

R206 impairs the binding of CoA

718888

C164Q

Pseudomonas aeruginosa

G3XDA2

site-directed mutagenesis, a mutant in which the binding site is altered to resemble the substrate-bound state

735397

C164A

Streptococcus pneumoniae

-

site-directed mutagenesis, inactive mutant

674581

C164A/H337A

Streptococcus pneumoniae

-

site-directed mutagenesis, inactive mutant

674581

C164A/K332A

Streptococcus pneumoniae

-

site-directed mutagenesis, inactive mutant

674581

E346A

Streptococcus pneumoniae

-

site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme

674581

E383A

Streptococcus pneumoniae

Q9FBC2

crystal structure determination and comparison to the wild-type enzyme, the mutation E383A appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form

701462

E396A

Streptococcus pneumoniae

-

site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity

674581

H303A

Streptococcus pneumoniae

-

site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme

674581

H337A

Streptococcus pneumoniae

-

site-directed mutagenesis, inactive mutant

674581

K332A

Streptococcus pneumoniae

-

site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity

674581

F107I

Streptomyces coelicolor

-

less efficient than wild type protein

719275

F107L

Streptomyces coelicolor

-

less efficient than wild type protein

719275

F107S

Streptomyces coelicolor

-

suggested that the Ser residue has less significant effects than the Ile and Leu mutations

719275

additional information

9 entries

additional information

Arabidopsis thaliana

-

construction of KASII knockout mutants by T-DNA disruption, knockout alleles fab1-1 and fab1-2, strong seed-specific hairpin-RNAi reductions in FAB1 expression resulted in abortion of about 1/4 of the embryos in an apparent phenocopy of fab1-2 homozygosity, in less severe FAB1 hairpin-RNAi individuals, embryos developed normally and exhibited a 1:2:1 segregation ratio for palmitate accumulation, thus, early embryo development appears sensitive to elevated 16:0, whereas at later stages, up to 53% of 16:0, i.e. a 7-fold increase over wild-type levels, is tolerated, Fab1-1 mutant plants show about 60% of wild-type enzyme activity and about 17% palm-like oil accumulation compared to the wild-type plants, phenotypes, overview

676894

additional information

Arabidopsis thaliana

Q9C9P4

construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview

703859

additional information

Arabidopsis thaliana

-

construction of a KAS2 T-DNA insertion mutant, the mutation causes arrest of embryonal development and embryo lethality, phenotype, overview

703859

additional information

Arabidopsis thaliana

Q9C9P4

transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs

737248

additional information

Clostridium acetobutylicum

-

expression of Clostridium acetobutylicium FabF1 restores thermal control of fatty acid composition to an Escherichia coli FabF null mutant strain. FabF1 expression leads a modest conversion of cis-3-decenoyl-[acyl-carrier-protein] to trans-2-cis-5-dodecadienoyl-[acyl carrier-protein]. An Escherichia coli fabF- strain in which Clostridium acetobutylicium FabF1 is expressed from the lac promoter of a low copy number vector closely mimicks the changes in fatty acid composition seen in wild type Escherichia coli strains upon changes in growth temperature. Expression of Clostridium acetobutylicium FabF1 restores cis-vaccenate synthesis at all temperatures, but is much more effective at 30°C than at 37°C or 42°C

702905

additional information

Elaeis guineensis

Q9M604

enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis

736673

additional information

Elaeis guineensis

-

enzyme overexpression in a strain deficient in palmitoyl-acyl carrier protein [ACP] thioesterase, EC 3.1.2.14, for improvement of palm oil production in Elaeis guineensis

736673

additional information

Nicotiana benthamiana

-

transient silencing of the KASII genes in Nicotiana benthamiana for metabolic engineering of wax ester composition, simultaneous inhibition of the two KASII genes Nicotiana benthamiana is possible using three different RNAi constructs

737248

additional information

Plasmodium falciparum

-

in vitro complementation of the enzyme-deficient Escherichia coli CY244 strain, shows that Plasmodium falciparum FabB/F functions like Escherichia coli FabF as the growth of the mutant cells can be rescued only in the presence of oleic acid

704117