2.3.1.179: beta-ketoacyl-[acyl-carrier-protein] synthase II

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For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase II, go to the full flat file.

Word Map on EC 2.3.1.179

2.3.1.179

cerulenin

malonyl-acp

acyl-acps

drug development

cis-vaccenic

stearoyl-acp

thiolactomycin

fasii

claisen

biotechnology

Reaction

a (Z)-hexadec-9-enoyl-[acyl-carrier protein]

a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein]

Synonyms

3-ketoacyl acyl synthase II, 3-ketoacyl-ACP synthase, 3-ketoacyl-ACP synthase 2, 3-ketoacyl-ACP synthase II, 3-oxoacyl-(acylcarrier protein) synthase II, 3-oxoacyl-ACP synthase II, B-ketoacyl-ACP synthase II, beta-ketoacyl ACP-synthase II, beta-ketoacyl acyl carrier protein synthase II, beta-ketoacyl acyl-carrier protein synthase II, beta-ketoacyl synthase II, beta-ketoacyl-(acyl-carrier-protein) synthase II, beta-ketoacyl-ACP synthase FabF3, beta-ketoacyl-ACP synthase II, beta-ketoacyl-acyl carrier protein synthase I/II, beta-ketoacyl-acyl carrier protein synthase II, beta-ketoacyl-acyl carrier protein synthases II, beta-ketoacyl-acyl carrier protein synthetase II, beta-ketoacyl-acyl-carrier protein synthase II, beta-ketoacyl-acyl-carrier-protein synthase II, beta-ketoacyl-[ACP] synthase II, beta-ketoacyl-[ACP] synthase-II, beta-ketoacyl-[acyl carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier protein (ACP)] synthase II, beta-ketoacyl-[acyl-carrier-protein] synthase II, FabB, FabB/F, FabF, FabF elongation condensing enzyme, FabF of type II fatty acid biosynthesis, FabF1, FASII, fatty acid synthesis type II, KAS II, KAS-II, KAS2, KASII

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.179 beta-ketoacyl-[acyl-carrier-protein] synthase II

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Results	in table
43692	AA Sequence
9	Application
1	CAS Registry Number
27	Cloned(Commentary)
11	Crystallization (Commentary)
1	Expression
23	General Information
1	General Stability
7	IC50 Value
70	Inhibitors
8	kcat/KM [mM/s]
23	KM Value [mM]
2	Localization
1	Metals/Ions
9	Molecular Weight [Da]
20	Natural Substrates/ Products (Substrates)
62	Organism
26	Pathway
44	PDB ID
5	pH Optimum
3	pI Value
27	Protein Variants
12	Purification (Commentary)
4	Reaction
1	Reaction Type
57	Reference
30	Source Tissue
3	Specific Activity [micromol/min/mg]
46	Substrates and Products (Substrate)
13	Subunits
104	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.179 - beta-ketoacyl-[acyl-carrier-protein] synthase II

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3 entries

immobilized metal ion affinity chromatography (Ni2+)

2 entries

Ni-NTA agarose column chromatography and HiLoad GF200 gel filtration

Chlamydia trachomatis

A0A0H3MGB3

755804

Ni-NTA Sepharose column chromatography and Superdex S200 gel filtration

2 entries

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinty chromatography, tag cleavage by TEV protease, gel filtration, and ultrafiltration to over 90% purity

Yersinia pestis

Q7CJ22

737251

recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinit chromatography, tag cleavage through TEV, dialysis, and gel filtration

Pseudomonas aeruginosa

G3XDA2

735397

recombinant wild-type and mutant His-tagged FabF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Streptococcus pneumoniae

674581

to homogeneity (>9000-fold purification), ion exchange chromatography (CM-Sepharose, HR-DEAE), hydroxyapatite and affinity chromatography (ACP-Sepharose)

Elaeis guineensis

720300