2.3.1.18: galactoside O-acetyltransferase

This is an abbreviated version!
For detailed information about galactoside O-acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyltransferase, galactoside, galactoside acetyltransferase, galactoside O-acetyltransferase, galactoside transacetylase, GAT, LacA, SACOL2570, thiogalactoside acetyltransferase, thiogalactoside transacetylase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.18 galactoside O-acetyltransferase

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Results	in table
902	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
1	Expression
2	General Information
11	Inhibitors
2	Ki Value [mM]
38	KM Value [mM]
3	Localization
3	Metals/Ions
8	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
27	Organism
12	PDB ID
2	pH Optimum
11	Protein Variants
9	Purification (Commentary)
4	Reaction
2	Reaction Type
16	Reference
5	Specific Activity [micromol/min/mg]
2	Storage Stability
59	Substrates and Products (Substrate)
6	Subunits
13	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
23	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.3.1.18 - galactoside O-acetyltransferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

4-nitrophenyl alpha-D-galactopyranoside + acetyl-CoA

4-nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside + CoA

Escherichia coli

-

-

486466

-

-

?

4-nitrophenyl beta-D-galactopyranoside + acetyl-CoA

4-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside + CoA

Escherichia coli

-

-

672425

-

-

?

acetyl-CoA + a beta-D-galactoside

CoA + a 6-acetyl-beta-D-galactoside

4 entries

acetyl-CoA + beta-D-galactoside

CoA + 6-acetyl-beta-D-galactoside

Escherichia coli

-

-

672425

-

-

?

acetyl-CoA + beta-D-methylgalactoside

CoA + 6-O-acetyl-beta-D-methylgalactoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + beta-D-phenylgalactoside

CoA + 6-O-acetyl-beta-D-phenylgalactoside

Escherichia coli

-

-

486461, 486466

-

-

?

acetyl-CoA + beta-D-phenylglucoside

CoA + 6-O-acetyl-beta-D-phenylglucoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + beta-D-thiodigalactoside

CoA + 6-O-acetyl-beta-D-thiodigalactoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + beta-D-thiophenylglucoside

CoA + 6-O-acetyl-beta-D-thiophenylglucoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + butyl beta-D-thiogalactoside

CoA + butyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

5 entries

acetyl-CoA + methyl beta-D-thiogalactoside

CoA + methyl 6-O-acetyl-beta-D-thiogalactoside

2 entries

acetyl-CoA + o-nitrophenyl-beta-D-galactopyranoside

CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside

Escherichia coli

-

-

486466

-

-

?

acetyl-CoA + o-nitrophenyl-beta-D-galactoside

CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside

Escherichia coli

-

-

486462

-

-

?

acetyl-CoA + o-nitrophenyl-beta-D-thiogalactoside

CoA + o-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486462

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-galactopyranoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside

Escherichia coli

-

-

486466

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-galactoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside

2 entries

acetyl-CoA + p-nitrophenyl-beta-D-glucopyranoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-glucopyranoside

Escherichia coli

-

-

486466

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-glucoside

CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside

2 entries

acetyl-CoA + p-nitrophenyl-beta-D-lactopyranoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactopyranoside

Escherichia coli

-

-

486466

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-lactoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactoside

Escherichia coli

-

-

486462

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-thiogalactoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486462

-

-

?

acetyl-CoA + phenyl beta-D-thiogalactoside

CoA + 6-O-acetyl-beta-D-thiophenylgalactoside

Escherichia coli

-

-

486461

-

-

?

butyryl-CoA + p-nitrophenyl-beta-D-galactopyranoside

CoA + p-nitrophenyl 6-O-butyryl-beta-D-galactopyranoside

Escherichia coli

-

-

486466

-

-

?

isopropyl-thio-beta-D-galactopyranoside

?

Escherichia coli

-

-

672425

-

-

?

isopropyl-thio-beta-D-galactopyranoside + acetyl-CoA

6-acetyl-isopropyl-thio-beta-D-galactopyranoside + CoA

Escherichia coli

-

-

672425

-

-

?

isopropyl-thio-beta-D-galactopyranoside + acetyl-CoA

isopropyl 6-O-acetyl-1-thio-beta-D-galactopyranoside + CoA

Escherichia coli

-

-

672425

-

-

?

p-nitrophenyl-beta-D-galactopyranoside

?

Escherichia coli

-

-

672425

-

-

?

p-nitrophenyl-beta-D-galactopyranoside + acetyl-CoA

p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside + CoA

Escherichia coli

-

-

672425

-

-

?

propionyl-CoA + p-nitrophenyl-beta-D-galactopyranoside

CoA + p-nitrophenyl 6-O-propionyl-beta-D-galactopyranoside

Escherichia coli

-

-

486466

-

-

?

additional information

?

-

14 entries

acetyl-CoA + a beta-D-galactoside

CoA + a 6-acetyl-beta-D-galactoside

Escherichia coli

-

-

658366, 672425

-

-

?

acetyl-CoA + a beta-D-galactoside

CoA + a 6-acetyl-beta-D-galactoside

Escherichia coli

-

prevents accumulation of methyl-1-thio-beta-D-galactoside, serves as a backup device to avoid metabolic congestion

658366

-

-

?

acetyl-CoA + a beta-D-galactoside

CoA + a 6-acetyl-beta-D-galactoside

Staphylococcus aureus

Q5HCZ5

galactoside acetyltransferases are enzymes that transfer an acetyl group from acetyl coen-zyme A to beta-galactosides. The enzymes have a broad substrate specificity and can acetylate many galactoside derivatives, including thiogalactosides and lactosides

736706

-

-

?

acetyl-CoA + a beta-D-galactoside

CoA + a 6-acetyl-beta-D-galactoside

Staphylococcus aureus

Q5HCZ5

CoA binds in the active site formed by the residues from adjacent LbetaH domains. After determination of CoA-bound structure, molecular dynamics simulations are performed to model the binding of AcCoA. Binding of both AcCoA and CoA to SACOL2570 is verified by isothermal titration calorimetry

736706

binding of CoA in the 3V4E crystal structure involves residues from two adjacent monomers in the homotrimeric assembly. Residues responsible for CoA binding by SACOL2570 are Asn84, Ala112, Gly141, Lys165, and Arg182

-

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486460, 486461

-

-

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486462

-

486462

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486464

-

-

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli A324-5

-

-

486462

-

486462

?

acetyl-CoA + isopropyl beta-D-thiogalactoside

CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli ML308

-

-

486460, 486461, 486464

-

-

?

acetyl-CoA + methyl beta-D-thiogalactoside

CoA + methyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli

-

-

486461

-

-

?

acetyl-CoA + methyl beta-D-thiogalactoside

CoA + methyl 6-O-acetyl-beta-D-thiogalactoside

Escherichia coli ML308

-

-

486461

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-galactoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside

Escherichia coli

-

twice as effective as isopropyl-beta-D-thiogalactoside

486462

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-galactoside

CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside

Escherichia coli A324-5

-

twice as effective as isopropyl-beta-D-thiogalactoside

486462

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-glucoside

CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside

Escherichia coli

-

-

486462

-

-

?

acetyl-CoA + p-nitrophenyl-beta-D-glucoside

CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside

Escherichia coli A324-5

-

-

486462

-

-

?

additional information

?

-

Escherichia coli

-

palmitoyl-CoA

486461

-

-

?

additional information

?

-

Escherichia coli

-

inactive: succinyl-CoA

486461

-

-

?

additional information

?

-

Escherichia coli

-

crotonyl-CoA

486462

-

-

?

additional information

?

-

Escherichia coli

-

oleoyl-CoA

486462

-

-

?

additional information

?

-

Escherichia coli

-

enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside

486466

-

-

?

additional information

?

-

Escherichia coli

-

enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside

486467

-

-

?

additional information

?

-

Escherichia coli

-

butyryl-CoA is about 10% as effective as acetyl-CoA, enzyme catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides but not or at much slower rate to galactosides except when a phenyl group is attached to the oxygen atom at carbon 1, a phenyl group substitution also allows glucosides to react

486461

-

-

?

additional information

?

-

Escherichia coli

-

it is suggested that the enzyme plays an important role in lactose utilization

486464

-

-

?

additional information

?

-

Escherichia coli A324-5

-

crotonyl-CoA

486462

-

-

?

additional information

?

-

Escherichia coli A324-5

-

oleoyl-CoA

486462

-

-

?

additional information

?

-

Escherichia coli ML308

-

palmitoyl-CoA

486461

-

-

?

additional information

?

-

Escherichia coli ML308

-

inactive: succinyl-CoA

486461

-

-

?

additional information

?

-

Escherichia coli ML308

-

butyryl-CoA is about 10% as effective as acetyl-CoA, enzyme catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides but not or at much slower rate to galactosides except when a phenyl group is attached to the oxygen atom at carbon 1, a phenyl group substitution also allows glucosides to react

486461

-

-

?

additional information

?

-

Escherichia coli ML308

-

it is suggested that the enzyme plays an important role in lactose utilization

486464

-

-

?